Human TNF RI/TNFRSF1A Antibody

Catalog # Availability Size / Price Qty
TNF RI/TNFRSF1A in Human Peripheral Blood Lymphocytes.
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Product Details
Citations (8)
Supplemental Products

Human TNF RI/TNFRSF1A Antibody Summary

Species Reactivity
Detects human sTNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse TNF sRI and recombinant rat TNF sRI is observed. This anti-human TNF RI antibody can interact with the cell surface 55 kDa human TNF RI and exhibits TNF agonist activities on the A549 human lung carcinoma cell line. This antibody also exhibits TNF agonist activities on the L‑929 mouse fibroblast cell line, suggesting that it has cross-species activity with mouse cell surface TNF RI.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human sTNF RI/TNFRSF1A
Accession # NP_001056
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1)
Flow Cytometry
2.5 µg/106 cells
Human blood-derived monocytes
Agonist Activity
Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
The ED50 for this effect is typically 1-6 μg/mL.
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry TNF RI/TNFRSF1A in Human Peripheral Blood Lymphocytes. View Larger

TNF RI/TNFRSF1A in Human Peripheral Blood Lymphocytes. TNF RI/TNFRSF1A was detected in immersion fixed human peripheral blood lymphocytes using 5 µg/mL Human TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF225) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RI/TNFRSF1A

TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.

Long Name
Tumor Necrosis Factor Receptor I
Entrez Gene IDs
7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

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Citations for Human TNF RI/TNFRSF1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Structural insights into the disruption of TNF-TNFR1 signalling by small molecules stabilising a distorted TNF
    Authors: D McMillan, C Martinez-F, J Porter, D Fox, R Davis, P Mori, T Ceska, B Carrington, A Lawson, T Bourne, J O'Connell
    Nature Communications, 2021;12(1):582.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  2. Small molecules that inhibit TNF signalling by stabilising an asymmetric form of the trimer
    Authors: J O'Connell, J Porter, B Kroeplien, T Norman, S Rapecki, R Davis, D McMillan, T Arakaki, A Burgin, D Fox Iii, T Ceska, F Lecomte, A Maloney, A Vugler, B Carrington, BP Cossins, T Bourne, A Lawson
    Nat Commun, 2019;10(1):5795.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. CAR T Cells Targeting MISIIR for the Treatment of Ovarian Cancer and Other Gynecologic Malignancies
    Authors: A Rodriguez-, P Sharma, M Poussin, AC Boesteanu, NG Minutolo, SB Gitto, DK Omran, MK Robinson, GP Adams, F Simpkins, DJ Powell
    Mol. Ther., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. NKp46+ Innate Lymphoid Cells Dampen Vaginal CD8 T Cell Responses following Local Immunization with a Cholera Toxin-Based Vaccine.
    Authors: Luci C, Bekri S, Bihl F, Pini J, Bourdely P, Nouhen K, Malgogne A, Walzer T, Braud V, Anjuere F
    PLoS ONE, 2015;10(12):e0143224.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-Fr
  5. Ubiquitin-associated domain-containing ubiquitin regulatory X (UBX) protein UBXN1 is a negative regulator of nuclear factor kappaB (NF-kappaB) signaling.
    Authors: Wang Y, Tan B, Mu R, Chang Y, Wu M, Tu H, Zhang Y, Guo S, Qin X, Li T, Li W, Li A, Zhang X, Li H
    J Biol Chem, 2015;290(16):10395-405.
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunoprecipitation
  6. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012;378(1):72-80.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  7. Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
    Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
    Dig. Dis. Sci., 2007;52(10):2775-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Stent implantation in coronary porcine arteries is associated with early activation of TNFalpha and TNFalpha receptor II expression.
    Authors: Hadjadj S, Cloutier I, Geoffroy P, Tanguay JF
    Atherosclerosis, 2007;192(1):25-32.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC-P


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