Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 488410
View all TPT1/TCTP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TPT1/TCTP
Met1-Cys172
Accession # P13693

Specificity

Detects human TPT1/TCTP in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human TPT1/TCTP Antibody

TPT1/TCTP antibody in Human Squamous Cell Carcinoma by Immunohistochemistry (IHC-P).

TPT1/TCTP in Human Squamous Cell Carcinoma.

TPT1/TCTP was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma of the lung using Mouse Anti-Human TPT1/TCTP Monoclonal Antibody (Catalog # MAB46481) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human TPT1/TCTP Antibody

Application
Recommended Usage

Immunohistochemistry

0.1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human squamous cell carcinoma of the lung

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TPT1/TCTP

TPT1 (translationally controlled tumor protein 1; also TCTP, HRF and fortilin) is a 23 kDa member of the TCTP family of calcium‑binding proteins. It is widely expressed (but not in kidney), and apparently serves as a transcriptional activator, calcium transporter, histamine inducer and antiapoptotic, caspase-3 inhibitor. Human TPT1 is 172 amino acids (aa) in length, with multiple potential splice variants. There is an alternate start site at Met35 that may be accompanied by a deletion of aa 99‑172. In addition, there may be either a 25 aa or 16 aa C-terminal extension after Cys172. Over aa 1‑172, human TPT1 is 96% aa identical to mouse TPT1.

Long Name

Translationally-controlled Tumor Protein 1

Alternate Names

Fortilin, HRF, p02, TCTP

Entrez Gene IDs

7178 (Human); 22070 (Mouse); 116646 (Rat)

Gene Symbol

TPT1

UniProt

P13693

Additional TPT1/TCTP Products

Product Documents for Human TPT1/TCTP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TPT1/TCTP Antibody

For research use only

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Protocols

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