Human TPT1/TCTP Antibody

Catalog # Availability Size / Price Qty
MAB46481-100
MAB46481-SP
Human TPT1/TCTP Antibody in Immunohistochemistry
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Human TPT1/TCTP Antibody Summary

Species Reactivity
Human
Specificity
Detects human TPT1/TCTP in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 488410
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TPT1/TCTP
Met1-Cys172
Accession # P13693
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
0.1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry TPT1/TCTP in Human Squamous Cell Carcinoma. View Larger

TPT1/TCTP in Human Squamous Cell Carcinoma. TPT1/TCTP was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma of the lung using Mouse Anti-Human TPT1/TCTP Monoclonal Antibody (Catalog # MAB46481) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TPT1/TCTP

TPT1 (translationally controlled tumor protein 1; also TCTP, HRF and fortilin) is a 23 kDa member of the TCTP family of calcium‑binding proteins. It is widely expressed (but not in kidney), and apparently serves as a transcriptional activator, calcium transporter, histamine inducer and antiapoptotic, caspase-3 inhibitor. Human TPT1 is 172 amino acids (aa) in length, with multiple potential splice variants. There is an alternate start site at Met35 that may be accompanied by a deletion of aa 99‑172. In addition, there may be either a 25 aa or 16 aa C-terminal extension after Cys172. Over aa 1‑172, human TPT1 is 96% aa identical to mouse TPT1.

Long Name
Translationally-controlled Tumor Protein 1
Entrez Gene IDs
7178 (Human); 22070 (Mouse); 116646 (Rat)
Alternate Names
FLJ27337; Fortilin; Histamine-releasing factor; HRF; p02; p23; TCTP; TCTPHRFfortilin; TPT1; translationally-controlled tumor protein; tumor protein, translationally-controlled 1

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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