Human TRAIL R3/TNFRSF10C Antibody

Catalog #: MAB6301
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6301-SP
MAB6301-500
MAB6301-100

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRAIL R3/TNFRSF10C Antibody Summary

Species Reactivity

Human

Specificity

Detects human TRAIL R3/TNFRSF10C in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) TRAIL R1, rhTRAIL R2, or rhTRAIL R4 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 90905

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TRAIL R3/TNFRSF10C
Ala26-Ala221
Accession # O14798

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human TRAIL R3/TNFRSF10C Fc Chimera (Catalog # 630-TR)

Immunohistochemistry

8-25 µg/mL

See below

Human TRAIL R3/TNFRSF10C Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human TRAIL R3/TNFRSF10C Biotinylated Antibody (Catalog # BAF630)

Standard: Recombinant Human TRAIL R3/TNFRSF10C Fc Chimera Protein (Catalog # 630-TR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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TRAIL R3/TNFRSF10C in Human Spleen. TRAIL R3/ TNFRSF10C was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human TRAIL R3/TNFRSF10C Monoclonal Antibody (Catalog # MAB6301) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAIL R3/TNFRSF10C

Human TRAIL R3, also called DcR1 (decoy receptor 1), LIT, and TRID and designated TNFRSF10C, is a glycosyl-phosphatidylinositol-linked membrane protein which binds TRAIL (Apo2 Ligand) with high affinity. TRAIL R3 has the TRAIL-binding extracellular cysteine-rich domains but lacks the intracellular signalling domain. As a result, binding of TRAIL to TRAIL R3 does not transduce an apoptosis signal. Expression of TRAIL R3 has been shown to protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-induced apoptosis. A second TRAIL decoy receptor, which binds TRAIL with high-affinity but antagonizes TRAIL-induced apoptosis, named TRAIL R4, DcR2 or TRUNDD, has also been reported. The human soluble TRAIL R3/Fc chimera neutralizes the ability of TRAIL to induce apoptosis.

References

Sheridan, J.P. et al. (1997) Science 277:818.
Golstein, P. (1997) Curr. Biol. 7:R750.

Long Name

TRAIL Receptor 3

Entrez Gene IDs

8794 (Human)

Alternate Names

Antagonist decoy receptor for TRAIL/Apo-2L; CD263 antigen; CD263; cytotoxic TRAIL receptor-3; DcR1; DCR1MGC149502; Decoy receptor 1; Decoy TRAIL receptor without death domain; LITMGC149501; Lymphocyte inhibitor of TRAIL; TNFRSF10C; TRAIL R3; TRAIL receptor 3; TRAIL receptor without an intracellular domain; TRAILR3; TRAIL-R3; TRAILR3TNF-related apoptosis-inducing ligand receptor 3; TRIDDCR1-TNFR; tumor necrosis factor receptor superfamily member 10C; tumor necrosis factor receptor superfamily, member 10c, decoy without anintracellular domain

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Citation for Human TRAIL R3/TNFRSF10C Antibody

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