Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 488915
View all TSC1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TSC1
Asp156-Thr300
Accession # Q92574

Specificity

Detects human TSC1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) TSC2 (aa 550-850), rhTSC2 (aa 1506-1748), or recombinant mouse TSC22 (aa 1-143) is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human TSC1 Antibody

Detection of Human TSC1 antibody by Western Blot.

Detection of Human TSC1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC-3 human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human TSC1 Monoclonal Antibody (Catalog # MAB4379) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TSC1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of TSC1 antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

Detection of TSC1 in Jurkat Human Cell Line by Flow Cytometry.

Jurkat human acute T cell leukemia cell line was stained with Human TSC1 Monoclonal Antibody (Catalog # MAB4379, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with PFA and permeabilized with saponin.

Applications for Human TSC1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with saponin.

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TSC1

TSC1 (Tuberous sclerosis 1), or hamartin, is a tumor suppressor which interacts with tumor suppressor TSC2 (tuberin) to form a cytoplasmic heterodimer. Mutations in either hamartin or tuberin are responsible for tuberous sclerosis (TSC), an autosomal dominant disease characterized by renal dysfunction, seizures, developmental delays, benign hamartomas and low grade neoplasms predominantly affecting the CNS, kidney, lung, skin, and heart. The TSC1/TSC2 complex suppresses cell growth by inhibiting mTOR, with TSC1 acting to inhibit the ubiquitination of TSC2, leading to increased cellular levels of TSC2 and thus enhancing its catalytic activity as a GTPase-activating protein for Rheb. TSC1 and TSC2 are also involved in the G2/M transition of the cell cycle through their interactions with CDK1 and cyclin B1. TSC1 has also been shown to interact with F-actin and ERM (Ezrin-Radixin-Moesin) proteins, implying a role in the modulation of cell adhesion and morphology.

Long Name

Tuberous Sclerosis 1

Alternate Names

Hamartin

Entrez Gene IDs

7248 (Human); 64930 (Mouse); 60445 (Rat)

Gene Symbol

TSC1

UniProt

Q92574

Additional TSC1 Products

Product Documents for Human TSC1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TSC1 Antibody

For research use only

Related Research Areas

Interferons

Citations for Human TSC1 Antibody

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