Human UNC5H3 Antibody

Catalog # Availability Size / Price Qty
MAB1005
MAB1005-SP
UNC5H3 in Normal Human Kidney.
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Product Details
Citations (1)
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Human UNC5H3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human UNC5H3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human UNC5H4 or recombinant rat UNC5H2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 200414
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human UNC5H3
Ala40-Asp376
Accession # O95185
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human UNC5H3 Fc Chimera (Catalog # 1005-UN)
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of normal human kidney 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry View Larger

UNC5H3 in Normal Human Kidney. UNC5H3 was detected in immersion fixed paraffin-embedded sections of normal human kidney (positive staining) and kidney cancer tissue (negative staining) using Mouse Anti-Human UNC5H3 Monoclonal Antibody (Catalog # MAB1005) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UNC5H3

Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues (including rat UNC5H1 and H2, mouse UNC5H2 and H3 (also known as rostral cerebellar malformation, RCM), and human UNC5H3 and H4) are transmembrane proteins belonging to the immunoglobulin (Ig) superfamily. All UNC5 family members have two Ig and two thrombospondin type 1 domains in their extracellular regions, as well as a conserved ZU-5 domain, a DCC (Deleted in Colorectal Cancer)-binding domain (DB) and a C-terminal death domain (DD) in their cytoplasmic regions (1, 2). UNC5 family proteins are receptors for the Netrin/UNC6 (netr: Sanskrit for “one who guides”) family of secreted axon guidance cues that are Laminin-related proteins. Netrin family proteins can act as chemoattractants for some axons and as chemorepellents for others. Besides UNC5, Netrin family proteins also bind to the DCC family of type I transmembrane receptors that share sequence similarity with proteins of the NCAM family, and adenosine A2b receptor, a G protein-coupled seven-transmembrane receptor belonging to the adenosine receptor family (3, 4). In vitro, Netrin binding to DCC family receptors in the absence of UNC5 is associated with axon attraction. However, the DCC-mediated attraction to Netrin is converted to repulsion by binding of UNC5 to the DCC-Netrin complex. In vivo, the mechanisms of Netrin-dependent axon attraction and repulsion are more complex and may include UNC5-mediated repulsion that is independent of DCC (1, 5). Besides their roles in axon guidance and neuronal migration, the UNC5 and DCC families also act as dependence receptors and exert pro-apoptotic effects in the absence of Netrin (6). Human UNC5H3 cDNA encodes a 931 amino acid (aa) type I membrane protein with a putative 39 aa signal peptide and 337 aa extracellular domain. The extracellular domain of human UNC5H3 shares approximately 98%, 73%, and 66% aa sequence similarity with mouse UNC5H3, rat UNC5H2, and human UNC5H4, respectively.

References
  1. Hong, K. et al. (1999) Cell 97:927.
  2. Leonardo, E.D. et al. (1997) Nature 386:833.
  3. Culotti, J.B. and D.C. Merz (1998) Curr. Opin. Cell Biol. 10:609.
  4. Corset, V. (2000) Nature 407:747.
  5. Merz, D.C. (2001) Genetics 158:1071.
  6. Llambi, F. et al. (2001) EMBO J. 20:2715.
Long Name
Netrin Receptor UNC5C
Entrez Gene IDs
8633 (Human)
Alternate Names
netrin receptor UNC5C; Protein unc-5 homolog 3; Protein unc-5 homolog C; unc-5 homolog 3; unc-5 homolog C (C. elegans); UNC5C; UNC5H3; UNC5H3unc5 (C.elegans homolog) c

Product Datasheets

Citation for Human UNC5H3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Methylation of the UNC5C gene and its protein expression in colorectal cancer
    Authors: J Wu, G Wang, B He, X Chen, Y An
    Tumour Biol., 2017;39(4):1010428317697.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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