Human UTF1 Antibody

Catalog # Availability Size / Price Qty
AF3958
AF3958-SP

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UTF1 in BG01V Human Embryonic Stem Cells.
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Human UTF1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human UTF1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human UTF1
Ser44-Lys153
Accession # Q5T230
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human UTF1
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry UTF1 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

UTF1 in BG01V Human Embryonic Stem Cells. UTF1 was detected in immersion fixed BG01V human embryonic stem cells using Goat Anti-Human UTF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3958) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001). SSEA4 was also detected using Mouse Anti-Human SSEA4 Monoclonal Antibody (Catalog # MAB1435) and stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Cells were counterstained with DAPI (blue). Specific staining of UTF1 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UTF1

UTF1 (undifferentiated embryonic cell transcription factor 1) is a 47‑57 kDa transcriptional coactivator. It is expressed mainly in embryonic stem cells, is regulated by Oct-3/4, and promotes rapid cell proliferation. Human UTF1 is a 341 amino acid (aa) phosphoprotein. It is rich in Proline and Arginine residues and contains a
C‑terminal Leu-zipper (aa 286‑313) that is known to interact with the metal-binding motif of ATF-2. Unlike mouse UTF1 which contains an alternate start site at Met43, human UTF1 lacks this Methionine and shows only one isoform. Over amino acids 44‑153, human UTF1 shares 81% aa sequence identity with mouse UTF1.

Long Name
Undifferentiated Embryonic Cell Transcription Factor 1
Entrez Gene IDs
8433 (Human); 22286 (Mouse); 681901 (Rat)
Alternate Names
undifferentiated embryonic cell transcription factor 1; UTF1

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