|Detection of Human VAMP‑7 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human VAMP‑7 Monoclonal Antibody (Catalog # MAB6117) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAMP‑7 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Vesicle-associated membrane protein 7 (VAMP-7) is a 25 kDa, widely expressed, type IV transmembrane protein and member of the synaptobrevin family. Mature human VAMP-7 consists of a 187 aa cytoplasmic domain, a 21 aa transmembrane region, and an 11 aa vesicular region. The cytoplasmic domain contains a longin domain (aa 7‑110) and a v-SNARE coiled-coil homology domain (aa 125‑185). Two splicing variants produce three isoforms for human VAMP-7. Isoform 2 has a 116 aa substitution for aa 145‑220 found in isoform 1, and isoform 3 is missing the residues corresponding to aa 28‑68 in isoform 1. Human VAMP-7 shares 99%, 97%, and 95% aa sequence identity with bovine, mouse, and rat VAMP-7, respectively.
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