Human VAMP-7 Antibody Summary
Accession # P51809
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human VAMP‑7 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human VAMP-7 Monoclonal Antibody (Catalog # MAB6117) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAMP-7 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Vesicle-associated membrane protein 7 (VAMP-7) is a 25 kDa, widely expressed, type IV transmembrane protein and member of the synaptobrevin family. Mature human VAMP-7 consists of a 187 aa cytoplasmic domain, a 21 aa transmembrane region, and an 11 aa vesicular region. The cytoplasmic domain contains a longin domain (aa 7‑110) and a v-SNARE coiled-coil homology domain (aa 125‑185). Two splicing variants produce three isoforms for human VAMP-7. Isoform 2 has a 116 aa substitution for aa 145‑220 found in isoform 1, and isoform 3 is missing the residues corresponding to aa 28‑68 in isoform 1. Human VAMP-7 shares 99%, 97%, and 95% aa sequence identity with bovine, mouse, and rat VAMP-7, respectively.
Citation for Human VAMP-7 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Exophagy of annexin A2 via RAB11, RAB8A and RAB27A in IFN-?-stimulated lung epithelial cells
Authors: YD Chen, YT Fang, YL Cheng, CF Lin, LJ Hsu, SY Wang, R Anderson, CP Chang, YS Lin
Sci Rep, 2017;7(1):5676.
Sample Types: Cell Lysates
Applications: Western Blot
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