Vang-like Protein 1/VANGL1 in HeLa Human Cell Line. Vang-like Protein 1/VANGL1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human Vang-like Protein 1/VANGL1 Monoclonal Antibody (Catalog # MAB5476) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vang-like Protein 1/VANGL1
Human Van Gogh-like protein 1 (VANGL1; also Vang-like Protein 1, strabismus 2, loop tail protein 2 homolog, LPP2) is a 60 kDa (predicted) member of the Vang family of proteins. VANGL1 is 524 amino acids (aa) in length and contains four transmembrane regions and the C-terminal Ser/Thr-X-Val motif, but lacks a signal sequence. There are two isoforms for VANGL1. In isoform 2, residues 67-68 found in isoform 1 are deleted. Human VANGL1 shares 95% aa sequence identity with mouse VANGL1. The glycoprotein is ubiquitously expressed in normal tissues and is upregulated in many cancer cell lines. VANGL1 is predicted to be a tumor suppressor gene. Mutations in VANGL1 are associated with neural tube defects.
Entrez Gene IDs:
81839 (Human); 229658 (Mouse); 690366 (Rat)
KAI1 C-terminal interacting tetraspanin; Loop-tail protein 2 homolog; LPP2; MGC5338; STB2; STB2KITENIN; STBM2; Strabismus 2; Van Gogh-like protein 1; vang (van gogh, Drosophila)-like 1, vang, van gogh-like 1 (Drosophila); Vang like Protein 1; VANGL1; vang-like 1 (van gogh, Drosophila); vang-like protein 1
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