Human VCAM-1/CD106 Antibody

(22 citations)   
  • Species Reactivity
    Human
  • Specificity
    The antibody was screened using COS cells transfected with cDNAs for E-Selectin, VCAM-1, ICAM-1 and PECAM-1 and was shown to be specific for human VCAM-1/CD106.
  • Source
    Monoclonal Mouse IgG1 Clone # BBIG-V1
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Activated HUVEC human umbilical vein endothelial cells
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human VCAM‑1/CD106 (Catalog # 809-VR) under non-reducing conditions only
  • Immunoprecipitation
    10 µg/mL
    Radiolabelled activated HUVEC human umbilical vein endothelial cells, see our available Western blot detection antibodies
  • Adhesion Blockade
    The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human VCAM-1/CD106 (Catalog # ADP5, 2.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 30 µg/mL of the antibody.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
VCAM‑1/CD106 in HUVEC Cells.

VCAM‑1/CD106 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # BBA5) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. This antibody is not suitable for labeling formalin-fixed, paraffin-embedded tissue.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VCAM-1/CD106
Vascular cell adhesion molecule 1 (VCAM-1) is expressed on the surface of activated endothelial cells and macrophages. It binds to leukocyte integrins VLA-4 and alpha 4 beta 7 to promote leukocyte adhesion at sites of inflamed vasculature.
  • Long Name:
    Vascular Cell Adhesion Molecule 1
  • Entrez Gene IDs:
    7412 (Human); 22329 (Mouse)
  • Alternate Names:
    CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Technical Advance: New in vitro method for assaying the migration of primary B cells using an endothelial monolayer as substrate
    Authors: PJ Stewart-Hu, TP Szasz, ER Jaeger, MD Onken, JA Cooper, SC Morley
    J. Leukoc. Biol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. p38 MAP kinase inhibitor suppresses transforming growth factor-beta2-induced type 1 collagen production in trabecular meshwork cells.
    Authors: Inoue-Mochita M, Inoue T, Fujimoto T, Kameda T, Awai-Kasaoka N, Ohtsu N, Kimoto K, Tanihara H
    PLoS ONE, 2015;10(3):e0120774.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  3. West Nile virus-induced cell adhesion molecules on human brain microvascular endothelial cells regulate leukocyte adhesion and modulate permeability of the in vitro blood-brain barrier model.
    Authors: Roe, Kelsey, Orillo, Beverly, Verma, Saguna
    PLoS ONE, 2014;9(7):e102598.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor.
    Authors: Park, Su-Jung, Kim, Ki-Jo, Kim, Wan-Uk, Cho, Chul-Soo
    J Immunol, 2014;192(7):3003-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment.
    Authors: Lauridsen H, Pober J, Gonzalez A
    FASEB J, 2014;28(3):1166-80.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  6. Phenolic acid composition, antiatherogenic and anticancer potential of honeys derived from various regions in Greece.
    Authors: Spilioti E, Jaakkola M, Tolonen T, Lipponen M, Virtanen V, Chinou I, Kassi E, Karabournioti S, Moutsatsou P
    PLoS ONE, 2014;9(4):e94860.
    Species: Human
    Sample Type: Whole Cells
    Application: ELISA Capture
  7. Uveal melanoma cells utilize a novel route for transendothelial migration.
    Authors: Onken, Michael, Li, Jinmei, Cooper, John A
    PLoS ONE, 2014;9(12):e115472.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. GroEL1, a heat shock protein 60 of Chlamydia pneumoniae, impairs neovascularization by decreasing endothelial progenitor cell function.
    Authors: Lin, Yi-Wen, Huang, Chun-Yao, Chen, Yung-Hsi, Shih, Chun-Min, Tsao, Nai-Wen, Lin, Cheng-Ye, Chang, Nen-Chun, Tsai, Chien-Su, Tsai, Hsiao-Ya, Tsai, Jui-Chi, Huang, Po-Hsun, Li, Chi-Yuan, Lin, Feng-Yen
    PLoS ONE, 2013;8(12):e84731.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  9. Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
    J. Exp. Med., 2012;209(10):1813-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  10. IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival.
    Authors: Benito-Miguel M, Garcia-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibanez T, Bonilla-Hernan MG, De Ayala CP, Sanchez-Mateos P, Martin-Mola E, Miranda-Carus ME
    PLoS ONE, 2012;7(7):e40620.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  11. Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
    Authors: Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH
    J. Hepatol., 2009;51(4):734-49.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  12. Involvement of native TRPC3 proteins in ATP-dependent expression of VCAM-1 and monocyte adherence in coronary artery endothelial cells.
    Authors: Smedlund K, Vazquez G
    Arterioscler. Thromb. Vasc. Biol., 2008;28(11):2049-55.
    Species: Human
    Sample Type: Whole Cells
    Application: ELISA Development
  13. Reversible commitment to differentiation by human multipotent stromal cells in single-cell-derived colonies.
    Authors: Ylostalo J, Bazhanov N, Prockop DJ
    Exp. Hematol., 2008;36(10):1390-402.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  14. Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption.
    Authors: Rajesh M, Mukhopadhyay P, Batkai S, Hasko G, Liaudet L, Drel VR, Obrosova IG, Pacher P
    Am. J. Physiol. Heart Circ. Physiol., 2007;293(1):H610-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  15. Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation.
    Authors: Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B
    Blood, 2007;109(7):2806-14.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  16. LIGHT up-regulated on B lymphocytes and monocytes in rheumatoid arthritis mediates cellular adhesion and metalloproteinase production by synoviocytes.
    Authors: Kang YM, Kim SY, Kang JH
    Arthritis Rheum., 2007;56(4):1106-17.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  17. SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration.
    Authors: Kelly KA, Allport JR, Yu AM, Sinh S, Sage EH, Gerszten RE, Weissleder R
    J. Leukoc. Biol., 2007;81(3):748-56.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  18. Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.
    Authors: Ramachandran R, Morice AH, Compton SJ
    Am. J. Respir. Cell Mol. Biol., 2006;35(1):133-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  19. The adaptor molecule Lnk negatively regulates tumor necrosis factor-alpha-dependent VCAM-1 expression in endothelial cells through inhibition of the ERK1 and -2 pathways.
    Authors: Fitau J, Boulday G, Coulon F, Quillard T, Charreau B
    J. Biol. Chem., 2006;281(29):20148-59.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  20. CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1.
    Authors: Yamamoto H, Nagata M, Sakamoto Y
    Ann. Allergy Asthma Immunol., 2005;94(2):292-300.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  21. Ligation of ICAM-1 on endothelial cells leads to expression of VCAM-1 via a nuclear factor-kappaB-independent mechanism.
    Authors: Lawson C, Ainsworth M, Yacoub M, Rose M
    J. Immunol., 1999;162(5):2990-6.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  22. Functions of domain 1 and 4 of vascular cell adhesion molecule-1 in alpha4 integrin-dependent adhesion under static and flow conditions are differentially regulated.
    Authors: Abe Y, Ballantyne CM, Smith CW
    J. Immunol., 1996;157(11):5061-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
Expand to show all 22 Citations
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