Human VCAM-1/CD106 Antibody

Catalog # Availability Size / Price Qty
BBA5
VCAM‑1/CD106 in HUVEC Cells.
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Product Details
Citations (33)
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Human VCAM-1/CD106 Antibody Summary

Species Reactivity
Human
Specificity
The antibody was screened using COS cells transfected with cDNAs for E-Selectin, VCAM-1, ICAM-1 and PECAM-1 and was shown to be specific for human VCAM-1/CD106.
Source
Monoclonal Mouse IgG1 Clone # BBIG-V1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human VCAM‑1/CD106 (Catalog # 809-VR) under non-reducing conditions only
Immunoprecipitation
10 µg/mL
Radiolabelled activated HUVEC human umbilical vein endothelial cells, see our available Western blot detection antibodies
Adhesion Blockade
The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human VCAM-1/CD106 (Catalog # ADP5, 2.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 30 µg/mL of the antibody.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry VCAM-1/CD106 antibody in HUVEC Cells by Immunocytochemistry (ICC). View Larger

VCAM‑1/CD106 in HUVEC Cells. VCAM-1/CD106 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Human VCAM-1/CD106 Monoclonal Antibody (Catalog # BBA5) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.This antibody is not suitable for labeling formalin-fixed, paraffin-embedded tissue.

Immunocytochemistry View Larger

VCAM‑1/CD106 in HUVEC cells treated with TNF alpha (positive) and untreated HUVEC cells (negative). VCAM‑1/CD106 was detected in immersion fixed HUVEC cells treated with TNF alpha (positive) and untreated HUVEC cells (negative) using Mouse Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # BBA5) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry/ Immunofluorescence Detection of Human VCAM-1/CD106 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human VCAM-1/CD106 by Immunocytochemistry/Immunofluorescence CD151 mediates lymphocyte binding to human liver tissue and colocalizes with VCAM-1 in primary human liver endothelium. A: representative low- and high-power images of static Stamper–Woodruff adhesion assays of cell tracker green-labeled peripheral blood lymphocytes binding to human liver tissue demonstrating adhesion to the sinusoidal regions within the liver parenchyma; cell nuclei were stained with DAPI. Dashed lines outline a fibrous septum, and arrows highlight lymphocytes within the parenchyma. B: sections were treated with anti-CD151 antibody or appropriate class-matched control antibody, and the results of 4 independent experiments are shown as the number of adherent cells per low-power image. C: dual-color immunofluorescent staining of TNF-alpha -treated HSECs with CD151 (green), VCAM-1 (red), and DAPI (nucleus, blue). Insets: background fluorescence for each class-matched control antibody. Bar = 200 μm (A), 50 μm (B), 10 μm (C). *P < 0.05. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28473332), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VCAM-1/CD106

Vascular cell adhesion molecule 1 (VCAM-1) is expressed on the surface of activated endothelial cells and macrophages. It binds to leukocyte integrins VLA-4 and alpha 4 beta 7 to promote leukocyte adhesion at sites of inflamed vasculature.

Long Name
Vascular Cell Adhesion Molecule 1
Entrez Gene IDs
7412 (Human); 22329 (Mouse)
Alternate Names
CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1

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Citations for Human VCAM-1/CD106 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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  1. Zika Virus Infection Promotes Local Inflammation, Cell Adhesion Molecule Upregulation, and Leukocyte Recruitment at the Blood-Brain Barrier
    Authors: M Clé, C Desmetz, J Barthelemy, MF Martin, O Constant, G Maarifi, V Foulongne, K Bolloré, Y Glasson, F De Bock, M Blaquiere, L Dehouck, N Pirot, E Tuaillon, S Nisole, F Najioullah, P Van de Per, A Cabié, N Marchi, F Gosselet, Y Simonin, S Salinas
    MBio, 2020-08-04;11(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration
    Authors: SM Koura, M Salama, M El-Hussiny, MEA Khalil, A Lotfy, SA Hassan, SA Gad Elhak, MA Sobh
    Mol Med Rep, 2019-02-01;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Calcium oscillations coordinate feather mesenchymal cell movement by SHH dependent modulation of gap junction networks
    Authors: A Li, JH Cho, B Reid, CC Tseng, L He, P Tan, CY Yeh, P Wu, Y Li, RB Widelitz, Y Zhou, M Zhao, RH Chow, CM Chuong
    Nat Commun, 2018-12-18;9(1):5377.
  4. Leishmania braziliensis Subverts Necroptosis by Modulating RIPK3 Expression
    Authors: NF Luz, R Khouri, J Van Weyenb, DL Zanette, PP Fiuza, A Noronha, A Barral, VS Boaventura, DB Prates, FK Chan, BB Andrade, VM Borges
    Front Microbiol, 2018-09-28;9(0):2283.
  5. Stevioside Prevents Wear Particle-Induced Osteolysis by Inhibiting Osteoclastogenesis and Inflammatory Response via the Suppression of TAK1 Activation
    Authors: J Meng, C Zhou, B Hu, M Luo, Y Yang, Y Wang, W Wang, G Jiang, J Hong, S Li, H Wu, S Yan, W Yan
    Front Pharmacol, 2018-09-26;9(0):1053.
  6. Lineage dynamics of murine pancreatic development at single-cell resolution
    Authors: LE Byrnes, DM Wong, M Subramania, NP Meyer, CL Gilchrist, SM Knox, AD Tward, CJ Ye, JB Sneddon
    Nat Commun, 2018-09-25;9(1):3922.
  7. Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells
    Authors: AM Lager, OG Corradin, JM Cregg, MS Elitt, HE Shick, BLL Clayton, KC Allan, HE Olsen, M Madhavan, PJ Tesar
    Nat Commun, 2018-09-13;9(1):3708.
  8. Engineering a multicellular vascular niche to model hematopoietic cell trafficking
    Authors: SS Kotha, BJ Hayes, KT Phong, MA Redd, K Bomsztyk, A Ramakrishn, B Torok-Stor, Y Zheng
    Stem Cell Res Ther, 2018-03-23;9(1):77.
    Applications: Neutralization
  9. Recombinant Human Vimentin Binds to P-Selectin and Blocks Neutrophil Capture and Rolling on Platelets and Endothelium
    Authors: FW Lam, Q Da, B Guillory, MA Cruz
    J. Immunol., 2018-01-15;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Blocking
  10. Technical Advance: New in vitro method for assaying the migration of primary B cells using an endothelial monolayer as substrate
    Authors: PJ Stewart-Hu, TP Szasz, ER Jaeger, MD Onken, JA Cooper, SC Morley
    J. Leukoc. Biol., 2017-06-21;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Experimental identification and computational characterization of a novel extracellular metalloproteinase produced by Clostridium sordellii
    Authors: MJ Aldape, A Tao, DD Heeney, ER McIndoo, JM French, D Xu
    RSC Adv, 2017-03-02;7(23):13928-13938.
    Applications: Western Blot
  12. Tandem CAR T cells targeting HER2 and IL13Ralpha2 mitigate tumor antigen escape.
    Authors: Hegde M, Mukherjee M, Grada Z, Pignata A, Landi D, Navai S, Wakefield A, Fousek K, Bielamowicz K, Chow K, Brawley V, Byrd T, Krebs S, Gottschalk S, Wels W, Baker M, Dotti G, Mamonkin M, Brenner M, Orange J, Ahmed N
    J Clin Invest, 2016-07-18;126(8):3036-52.
  13. p38 MAP kinase inhibitor suppresses transforming growth factor-beta2-induced type 1 collagen production in trabecular meshwork cells.
    Authors: Inoue-Mochita M, Inoue T, Fujimoto T, Kameda T, Awai-Kasaoka N, Ohtsu N, Kimoto K, Tanihara H
    PLoS ONE, 2015-03-23;10(3):e0120774.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. Uveal melanoma cells utilize a novel route for transendothelial migration.
    Authors: Onken, Michael, Li, Jinmei, Cooper, John A
    PLoS ONE, 2014-12-15;9(12):e115472.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  15. West Nile virus-induced cell adhesion molecules on human brain microvascular endothelial cells regulate leukocyte adhesion and modulate permeability of the in vitro blood-brain barrier model.
    Authors: Roe, Kelsey, Orillo, Beverly, Verma, Saguna
    PLoS ONE, 2014-07-18;9(7):e102598.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Phenolic acid composition, antiatherogenic and anticancer potential of honeys derived from various regions in Greece.
    Authors: Spilioti E, Jaakkola M, Tolonen T, Lipponen M, Virtanen V, Chinou I, Kassi E, Karabournioti S, Moutsatsou P
    PLoS ONE, 2014-04-21;9(4):e94860.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development (Capture)
  17. Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor.
    Authors: Park, Su-Jung, Kim, Ki-Jo, Kim, Wan-Uk, Cho, Chul-Soo
    J Immunol, 2014-02-26;192(7):3003-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. GroEL1, a heat shock protein 60 of Chlamydia pneumoniae, impairs neovascularization by decreasing endothelial progenitor cell function.
    Authors: Lin, Yi-Wen, Huang, Chun-Yao, Chen, Yung-Hsi, Shih, Chun-Min, Tsao, Nai-Wen, Lin, Cheng-Ye, Chang, Nen-Chun, Tsai, Chien-Su, Tsai, Hsiao-Ya, Tsai, Jui-Chi, Huang, Po-Hsun, Li, Chi-Yuan, Lin, Feng-Yen
    PLoS ONE, 2013-12-23;8(12):e84731.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment.
    Authors: Lauridsen H, Pober J, Gonzalez A
    FASEB J, 2013-12-02;28(3):1166-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
    J. Exp. Med., 2012-09-17;209(10):1813-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival.
    Authors: Benito-Miguel M, Garcia-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibanez T, Bonilla-Hernan MG, De Ayala CP, Sanchez-Mateos P, Martin-Mola E, Miranda-Carus ME
    PLoS ONE, 2012-07-09;7(7):e40620.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
    Authors: Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH
    J. Hepatol., 2009-07-30;51(4):734-49.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC, Neutralization
  23. Involvement of native TRPC3 proteins in ATP-dependent expression of VCAM-1 and monocyte adherence in coronary artery endothelial cells.
    Authors: Smedlund K, Vazquez G
    Arterioscler. Thromb. Vasc. Biol., 2008-09-11;28(11):2049-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development
  24. Reversible commitment to differentiation by human multipotent stromal cells in single-cell-derived colonies.
    Authors: Ylostalo J, Bazhanov N, Prockop DJ
    Exp. Hematol., 2008-07-10;36(10):1390-402.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  25. Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation.
    Authors: Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B
    Blood, 2007-04-01;109(7):2806-14.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. LIGHT up-regulated on B lymphocytes and monocytes in rheumatoid arthritis mediates cellular adhesion and metalloproteinase production by synoviocytes.
    Authors: Kang YM, Kim SY, Kang JH
    Arthritis Rheum., 2007-04-01;56(4):1106-17.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption.
    Authors: Rajesh M, Mukhopadhyay P, Batkai S, Hasko G, Liaudet L, Drel VR, Obrosova IG, Pacher P
    Am. J. Physiol. Heart Circ. Physiol., 2007-03-23;293(1):H610-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration.
    Authors: Kelly KA, Allport JR, Yu AM, Sinh S, Sage EH, Gerszten RE, Weissleder R
    J. Leukoc. Biol., 2006-12-18;81(3):748-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  29. The adaptor molecule Lnk negatively regulates tumor necrosis factor-alpha-dependent VCAM-1 expression in endothelial cells through inhibition of the ERK1 and -2 pathways.
    Authors: Fitau J, Boulday G, Coulon F, Quillard T, Charreau B
    J. Biol. Chem., 2006-04-27;281(29):20148-59.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  30. Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.
    Authors: Ramachandran R, Morice AH, Compton SJ
    Am. J. Respir. Cell Mol. Biol., 2006-02-23;35(1):133-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1.
    Authors: Yamamoto H, Nagata M, Sakamoto Y
    Ann. Allergy Asthma Immunol., 2005-02-01;94(2):292-300.
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: ELISA Development, ICC
  32. Ligation of ICAM-1 on endothelial cells leads to expression of VCAM-1 via a nuclear factor-kappaB-independent mechanism.
    Authors: Lawson C, Ainsworth M, Yacoub M, Rose M
    J. Immunol., 1999-03-01;162(5):2990-6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  33. Functions of domain 1 and 4 of vascular cell adhesion molecule-1 in alpha4 integrin-dependent adhesion under static and flow conditions are differentially regulated.
    Authors: Abe Y, Ballantyne CM, Smith CW
    J. Immunol., 1996-12-01;157(11):5061-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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