Human VCAM‑1/CD106 Antibody

Name: Human VCAM‑1/CD106 Antibody
Brand: R&D Systems
SKU: BBA5
Price: 449 USD
Availability: InStock
Rating: 4 (1 reviews)

R&D Systems | Catalog # BBA5

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Rat, Insect - Aedes albopictus (Asian tiger mosquito)

Applications

Validated:

Western Blot, Adhesion Blockade, Immunocytochemistry, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Blocking, ELISA Development, ELISA Development (Capture), Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # BBIG-V1

View all VCAM-1/CD106 Primary Antibodies »

Product Specifications

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Specificity

The antibody was screened using COS cells transfected with cDNAs for E-Selectin, VCAM-1, ICAM-1 and PECAM-1 and was shown to be specific for human VCAM-1/CD106.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human VCAM‑1/CD106 Antibody

VCAM‑1/CD106 in HUVEC Cells.

VCAM-1/CD106 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Human VCAM-1/CD106 Monoclonal Antibody (Catalog # BBA5) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.This antibody is not suitable for labeling formalin-fixed, paraffin-embedded tissue.

VCAM‑1/CD106 in HUVEC cells treated with TNF alpha (positive) and untreated HUVEC cells (negative).

VCAM‑1/CD106 was detected in immersion fixed HUVEC cells treated with TNF alpha (positive) and untreated HUVEC cells (negative) using Mouse Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # BBA5) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human VCAM‑1/CD106 Antibody

Application

Recommended Usage

Adhesion Blockade

The adhesion of U937 human histiocytic lymphoma cells (5 x 10⁴ cells/well) to immobilized Recombinant Human VCAM-1/CD106 (Catalog # ADP5, 2.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 30 µg/mL of the antibody.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells, and immersion fixed HUVEC human umbilical vein endothelial cells activated with recombinant human TNF-alpha (Catalog # 210-TA). This antibody is not suitable for labeling formalin-fixed, paraffin-embedded tissue.

Immunoprecipitation

10 µg/mL
Sample: Radiolabelled activated HUVEC human umbilical vein endothelial cells, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human VCAM‑1/CD106 (Catalog # 809-VR) under non-reducing conditions only

Reviewed Applications

Read 1 review rated 4 using BBA5 in the following applications:

Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: VCAM-1/CD106

Vascular cell adhesion molecule 1 (VCAM-1) is expressed on the surface of activated endothelial cells and macrophages. It binds to leukocyte integrins VLA-4 and alpha 4 beta 7 to promote leukocyte adhesion at sites of inflamed vasculature.