Human VEGFR2/KDR/Flk-1 Antibody

Catalog # Availability Size / Price Qty
MAB3573-SP
MAB3573-500
MAB3573-100
Product Details
Citations (6)
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Human VEGFR2/KDR/Flk-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGFR2/KDR/Flk-1 in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) VEGFR1, rhVEGFR3, or recombinant mouse VEGFR2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 89109
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR2/KDR/Flk-1
Ala20-Glu764
Accession # P35968
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human VEGFR2/KDR/Flk-1 Fc Chimera (Catalog # 357-KD)
under non-reducing conditions only. It is recommended to use Goat Anti-Human VEGFR2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357).

Human VEGFR2/KDR Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human VEGFR2/KDR/Flk‑1 Biotinylated Antibody (Catalog # BAF357)

Standard: Recombinant Human VEGFR2/KDR Fc Chimera Protein (Catalog # 357-KD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR2/KDR/Flk-1

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGFR2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 567 aa residue cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

References
  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
Long Name
Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs
3791 (Human); 16542 (Mouse)
Alternate Names
CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2

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Citations for Human VEGFR2/KDR/Flk-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Discovering Small Molecule Ligands of Vascular Endothelial Growth Factor That Block VEGF–KDR Binding Using Label-Free Microarray-Based Assays
    Authors: James P. Landry, Yiyan Fei, Xiangdong Zhu, Yaohuang Ke, Guoliang Yu, Pierre Lee
    ASSAY and Drug Development Technologies
  2. Rhodocetin-alpha beta –induced Neuropilin-1–cMet Association Triggers Restructuring of Matrix Contacts in Endothelial Cells
    Authors: Stephan Niland, Bartosz Ditkowski, Désirée Parrandier, Lise Roth, Hellmut Augustin, Johannes A. Eble
    Arteriosclerosis, Thrombosis, and Vascular Biology
  3. Liposomal honokiol inhibits VEGF-D-induced lymphangiogenesis and metastasis in xenograft tumor model.
    Authors: Wen J, Fu AF, Chen LJ, Xie XJ, Yang GL, Chen XC, Wang YS, Li J, Chen P, Tang MH, Shao XM, Lu Y, Zhao X, Wei YQ
    Int. J. Cancer, 2009-06-01;124(11):2709-18.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  4. Down-regulation of vascular endothelial growth factor receptor 2 is a major molecular determinant of proteasome inhibitor-mediated antiangiogenic action in endothelial cells.
    Authors: Meissner M, Reichenbach G, Stein M, Hrgovic I, Kaufmann R, Gille J
    Cancer Res., 2009-02-17;69(5):1976-84.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells.
    Authors: Kobune M, Kawano Y, Takahashi S, Takada K, Murase K, Iyama S, Sato T, Takimoto R, Niitsu Y, Kato J
    Exp. Hematol., 2008-06-17;36(9):1121-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Suppression of angiogenesis and tumor growth by selective inhibition of angiopoietin-2.
    Authors: Oliner J, Min H, Leal J, Yu D, Rao S, You E, Tang X, Kim H, Meyer S, Han SJ, Hawkins N, Rosenfeld R, Davy E, Graham K, Jacobsen F, Stevenson S, Ho J, Chen Q, Hartmann T, Michaels M, Kelley M, Li L, Sitney K, Martin F, Sun JR, Zhang N, Lu J, Estrada J, Kumar R, Coxon A, Kaufman S, Pretorius J, Scully S, Cattley R, Payton M, Coats S, Nguyen L, Desilva B, Ndifor A, Hayward I, Radinsky R, Boone T, Kendall R
    Cancer Cell, 2004-11-01;6(5):507-16.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development

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