Human Wnt-7b Antibody

Catalog # Availability Size / Price Qty
AF3460
AF3460-SP
Detection of Human Wnt‑7b by Western Blot.
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Product Details
Citations (10)
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Human Wnt-7b Antibody Summary

Species Reactivity
Human
Specificity
Detects human Wnt-7b in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Wnt-7a is observed and less than 5% cross-reactivity with recombinant mouse (rm) Wnt-1, rmWnt-3a, rmWnt-4, rmWnt-5a, rhWnt‑9a, rmWnt-11, and rmWnt-16 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Wnt‑7b
Leu32-Glu72 & Thr216-Ala283
Accession # P56706
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Wnt-7b antibody by Western Blot. View Larger

Detection of Human Wnt‑7b by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Wnt-7b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3460) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Wnt-7b at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-7b

Wnt-7b is a 46 kDa, secreted glycosylated protein that belongs to the Wnt family. Wnt proteins can be lipid-modified and are ligands for members of the frizzled family of receptors, which mediates cell-cell communication during development. Human Wnt-7b is synthesized as a 349 amino acid (aa) precursor that contains a 318 aa mature region. The mature region contains 24 cysteines and three potential N-linked glycosylation sites. Mature human Wnt-7b shares 99% aa sequence identity with mature mouse and rat Wnt-7b. Wnt-7b also shares 80% aa sequence identity with Wnt-7a.

Long Name
Wingless-type MMTV Integration Site Family, Member 7B
Entrez Gene IDs
7477 (Human); 22422 (Mouse); 315196 (Rat)
Alternate Names
protein Wnt-7b; wingless-type MMTV integration site family, member 7B; Wnt7b; Wnt-7b

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Citations for Human Wnt-7b Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Epiblast inducers capture mouse trophectoderm stem cells in�vitro and pattern blastoids for implantation in utero
    Authors: J Seong, J Frias-Alde, V Holzmann, H Kagawa, G Sestini, H Heidari Kh, Y Scholte Op, M Kip, SJ Pradhan, L Verwegen, J Vivié, L Li, A Alemany, J Korving, F Darmis, A van Oudena, D Ten Berge, N Geijsen, NC Rivron
    Cell Stem Cell, 2022;29(7):1102-1118.e8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Fzd7/Wnt7b signaling contributes to stemness and chemoresistance in pancreatic cancer
    Authors: Z Zhang, Y Xu, C Zhao
    Cancer Medicine, 2021;10(10):3332-3345.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. LGR4 maintains HGSOC cell epithelial phenotype and stem-like traits
    Authors: Z Wang, P Yin, Y Sun, L Na, J Gao, W Wang, C Zhao
    Gynecol Oncol, 2020;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. p73 regulates epidermal wound healing and induced keratinocyte programming
    Authors: JS Beeler, CB Marshall, PI Gonzalez-E, TM Shaver, GL Santos Gua, ST Lea, KN Johnson, H Jin, BJ Venters, ME Sanders, JA Pietenpol
    PLoS ONE, 2019;14(6):e0218458.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. ABCB1 and ABCG2 drug transporters are differentially expressed in non-small cell lung cancers (NSCLC) and expression is modified by cisplatin treatment via altered Wnt signaling
    Authors: M Vesel, J Rapp, D Feller, E Kiss, L Jaromi, M Meggyes, G Miskei, B Duga, G Smuk, T Laszlo, I Karner, JE Pongracz
    Respir. Res, 2017;18(1):52.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation
    Authors: Bruce F Kimler
    J. Endocrinol., 2016;229(2):197-207.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Downregulated miR329 and miR410 promote the proliferation and invasion of oral squamous cell carcinoma by targeting Wnt-7b.
    Authors: Shiah S, Hsiao J, Chang W, Chen Y, Jin Y, Wong T, Huang J, Tsai S, Hsu Y, Chou S, Yen Y, Jiang S, Shieh Y, Chang I, Hsiao M, Chang J
    Cancer Res, 2014;74(24):7560-72.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. CD82 inhibits canonical Wnt signalling by controlling the cellular distribution of beta-catenin in carcinoma cells.
    Authors: Chigita S, Sugiura T, Abe M, Kobayashi Y, Shimoda M, Onoda M, Shirasuna K
    Int J Oncol, 2012;41(6):2021-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Parabronchial smooth muscle constitutes an airway epithelial stem cell niche in the mouse lung after injury.
    Authors: Volckaert T, Dill E, Campbell A, Tiozzo C, Majka S, Bellusci S, De Langhe SP
    J. Clin. Invest., 2011;121(11):4409-19.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus.
    Authors: Gogolla N, Galimberti I, Deguchi Y, Caroni P
    Neuron, 2009;62(4):510-25.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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