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Human Wnt-7b Antibody

Catalog #: AF3460
17 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3460
AF3460-SP

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Detection of Wnt‑7b in Human Breast Cancer
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Citations (17)
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Human Wnt-7b Antibody Summary

Species Reactivity
Human
Specificity
Detects human Wnt-7b in direct ELISAs.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Wnt‑7b
Leu32-Glu72 & Thr216-Ala283
Accession # P56706
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue and human breast cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Detection of Wnt‑7b in Human Breast Cancer Wnt‑7b was detected in immersion fixed paraffin-embedded sections of human breast cancer using Goat Anti-Human Wnt‑7b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3460) at 15 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm of cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-7b

Wnt-7b is a 46 kDa, secreted glycosylated protein that belongs to the Wnt family. Wnt proteins can be lipid-modified and are ligands for members of the frizzled family of receptors, which mediates cell-cell communication during development. Human Wnt-7b is synthesized as a 349 amino acid (aa) precursor that contains a 318 aa mature region. The mature region contains 24 cysteines and three potential N-linked glycosylation sites. Mature human Wnt-7b shares 99% aa sequence identity with mature mouse and rat Wnt-7b. Wnt-7b also shares 80% aa sequence identity with Wnt-7a.

Long Name
Wingless-type MMTV Integration Site Family, Member 7B
Entrez Gene IDs
7477 (Human); 22422 (Mouse); 315196 (Rat)
Alternate Names
protein Wnt-7b; wingless-type MMTV integration site family, member 7B; Wnt7b; Wnt-7b

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Citations for Human Wnt-7b Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Small Extracellular Vesicles Promote Axon Outgrowth by Engaging the Wnt-Planar Cell Polarity Pathway
    Authors: Ahmad, S;Christova, T;Pye, M;Narimatsu, M;Song, S;Wrana, JL;Attisano, L;
    Cells
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  2. Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer's disease, restores synapse integrity and memory in a disease mouse model
    Authors: Martin Flores, N;Podpolny, M;McLeod, F;Workman, I;Crawford, K;Ivanov, D;Leonenko, G;Escott-Price, V;Salinas, PC;
    eLife
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. RBL2-E2F-GCN5 guide cell fate decisions during tissue specification by regulating cell-cycle-dependent fluctuations of non-cell-autonomous signaling
    Authors: Militi, S;Nibhani, R;Jalali, M;Pauklin, S;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Epiblast inducers capture mouse trophectoderm stem cells in�vitro and pattern blastoids for implantation in utero
    Authors: J Seong, J Frias-Alde, V Holzmann, H Kagawa, G Sestini, H Heidari Kh, Y Scholte Op, M Kip, SJ Pradhan, L Verwegen, J Vivié, L Li, A Alemany, J Korving, F Darmis, A van Oudena, D Ten Berge, N Geijsen, NC Rivron
    Cell Stem Cell, 2022-07-07;29(7):1102-1118.e8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Activation of WNT7b autocrine eases metastasis of colorectal cancer via epithelial to mesenchymal transition and predicts poor prognosis
    Authors: Shuai Jiang, Qiwen Li, Yimin Liu, Huimin Zhang, Qianyu Wang, Yu Chen et al.
    BMC Cancer
  6. Fzd7/Wnt7b signaling contributes to stemness and chemoresistance in pancreatic cancer
    Authors: Z Zhang, Y Xu, C Zhao
    Cancer Medicine, 2021-05-02;10(10):3332-3345.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  7. LGR4 maintains HGSOC cell epithelial phenotype and stem-like traits
    Authors: Z Wang, P Yin, Y Sun, L Na, J Gao, W Wang, C Zhao
    Gynecol Oncol, 2020-09-24;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. p73 regulates epidermal wound healing and induced keratinocyte programming
    Authors: JS Beeler, CB Marshall, PI Gonzalez-E, TM Shaver, GL Santos Gua, ST Lea, KN Johnson, H Jin, BJ Venters, ME Sanders, JA Pietenpol
    PLoS ONE, 2019-06-19;14(6):e0218458.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  9. Fgf10-Hippo Epithelial-Mesenchymal Crosstalk Maintains and Recruits Lung Basal Stem Cells
    Authors: Thomas Volckaert, Tingting Yuan, Cho-Ming Chao, Harold Bell, Alina Sitaula, Luisa Szimmtenings et al.
    Developmental Cell
  10. ABCB1 and ABCG2 drug transporters are differentially expressed in non-small cell lung cancers (NSCLC) and expression is modified by cisplatin treatment via altered Wnt signaling
    Authors: M Vesel, J Rapp, D Feller, E Kiss, L Jaromi, M Meggyes, G Miskei, B Duga, G Smuk, T Laszlo, I Karner, JE Pongracz
    Respir. Res, 2017-03-24;18(1):52.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation
    Authors: Bruce F Kimler
    J. Endocrinol., 2016-03-14;229(2):197-207.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Expression of WNT5A in Idiopathic Pulmonary Fibrosis and Its Control by TGF-beta and WNT7B in Human Lung Fibroblasts
    Authors: Donna R. Newman, W. Shane Sills, Katherine Hanrahan, Amanda Ziegler, Kathleen McGinnis Tidd, Elizabeth Cook et al.
    Journal of Histochemistry & Cytochemistry
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Downregulated miR329 and miR410 promote the proliferation and invasion of oral squamous cell carcinoma by targeting Wnt-7b.
    Authors: Shiah S, Hsiao J, Chang W, Chen Y, Jin Y, Wong T, Huang J, Tsai S, Hsu Y, Chou S, Yen Y, Jiang S, Shieh Y, Chang I, Hsiao M, Chang J
    Cancer Res, 2014-10-28;74(24):7560-72.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  14. Role of WNT7B-induced Noncanonical Pathway in Advanced Prostate Cancer
    Authors: Dali Zheng, Keith F. Decker, Tianhua Zhou, Jianquan Chen, Zongtai Qi, Kathryn Jacobs et al.
    Molecular Cancer Research
  15. CD82 inhibits canonical Wnt signalling by controlling the cellular distribution of beta-catenin in carcinoma cells.
    Authors: Chigita S, Sugiura T, Abe M, Kobayashi Y, Shimoda M, Onoda M, Shirasuna K
    Int J Oncol, 2012-10-17;41(6):2021-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Parabronchial smooth muscle constitutes an airway epithelial stem cell niche in the mouse lung after injury.
    Authors: Volckaert T, Dill E, Campbell A, Tiozzo C, Majka S, Bellusci S, De Langhe SP
    J. Clin. Invest., 2011-10-10;121(11):4409-19.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus.
    Authors: Gogolla N, Galimberti I, Deguchi Y, Caroni P
    Neuron, 2009-05-28;62(4):510-25.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot

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