Detects human Wnt-7b in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Wnt-7a is observed and less than 5% cross-reactivity with recombinant mouse (rm) Wnt-1, rmWnt-3a, rmWnt-4, rmWnt-5a, rhWnt‑9a, rmWnt-11, and rmWnt-16 is observed.
Polyclonal Goat IgG
E. coli-derived recombinant human Wnt‑7b Leu32-Glu72 & Thr216-Ala283 Accession # P56706
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Wnt‑7b by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Wnt‑7b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3460) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Wnt‑7b at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Wnt-7b is a 46 kDa, secreted glycosylated protein that belongs to the
Wnt family. Wnt proteins can be lipid-modified and are ligands for
members of the frizzled family of receptors, which mediates cell-cell
communication during development. Human Wnt-7b is synthesized as a 349
amino acid (aa) precursor that contains a 318 aa mature region. The
mature region contains 24 cysteines and three potential N-linked
glycosylation sites. Mature human Wnt-7b shares 99% aa sequence
identity with mature mouse and rat Wnt-7b. Wnt-7b also shares 80% aa
sequence identity with Wnt-7a.
Wingless-type MMTV Integration Site Family, Member 7B
Entrez Gene IDs:
7477 (Human); 22422 (Mouse); 315196 (Rat)
protein Wnt-7b; wingless-type MMTV integration site family, member 7B; Wnt7b
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.