Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ACE-2 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse ACE-2
Gln18-Thr740
Accession # Q8R0I0

Specificity

Detects mouse ACE-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant rat ACE‑2 and 10% cross-reactivity with recombinant human ACE-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse ACE‑2 Antibody

Detection of Mouse ACE-2 antibody by Western Blot.

Detection of Mouse ACE‑2 by Western Blot.

Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ACE-2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of ACE-2 antibody in HEK293 Human Cell Line Transfected with Mouse ACE-2 and eGFP antibody by Flow Cytometry.

Detection of ACE-2 in HEK293 Human Cell Line Transfected with Mouse ACE-2 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) mouse ACE-2 or (B) irrelevant protein, and eGFP was stained with Goat Anti-Mouse ACE-2 Affinity Purified Polyclonal Antibody (Catalog # AF3437) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). Quadrant markers were set based on Goat IgG control antibody (AB-108-C, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

ACE‑2 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr).

ACE‑2 in Mouse Kidney.

ACE‑2 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

ACE-2 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr).

ACE‑2 in Mouse Kidney.

ACE-2 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse ACE-2 antibody by Simple WesternTM.

Detection of Mouse ACE‑2 by Simple WesternTM.

Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE-2 at approximately 141 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Mouse ACE‑2 ELISA Standard Curve.

Recombinant Mouse ACE‑2 protein was serially diluted 2-fold and captured by Rat Anti-Mouse ACE‑2 Monoclonal Antibody (MAB34371) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Detection of ACE-2 by Western Blot

Detection of ACE-2 by Western Blot

Expression of vasoactive proteins (mRNA and protein levels) in response to different oxygen conditions (mRNA: fold change relative to control condition: 21% O2; protein: % of control condition: 21% O2) as determined by qRT‐PCR using actb as house‐keeping gene and the delta delta Ct method and western blotting from cell lysates. (a) NOS3 mRNA expression, protein expression at 72 hr and Griess reaction (analyzed levels of NO2− and NO3− in the supernatants resulting from NOS activity). (b) ACE1, ACE2 mRNA expression and ACE1, ACE2 intracellular protein expression at 72 hr. (c) ACE1 and ACE2 protein levels in the cell culture supernatant as determined by Western blotting and mRNA expression of sheddases ADAM10 and ADAM17. p‐values result from statistical testing with Student's t‐test (mRNA), one‐way ANOVA (protein) and two‐way ANOVA (Griess) with Tukey's multiple comparison test. *p < .05; **p < .01; ***p < .001, ****p < .0001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33565273), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of ACE-2 by Flow Cytometry

Detection of ACE-2 by Flow Cytometry

regDCs EXO are taken up by acceptor PBTECs, inhibiting ACE2 expression in vitro. (A) Uptake of Dil labeled EXO (red) by PBTECs, counterstained with nuclear stain DAPI (blue), phalloidin (Alex flour 647) for cell membrane and visualized under confocal microscopy. ACE2 mRNA expression (B) and flow cytometry scattergrams showing ACE2 positive cells percentage (C) in PBTECs treated or not treated with iDCs or regDCs EXO in the presence or absence of TGF beta R1 inhibitor SB431542. (D) representative bar graph of (C). Results shown are representative of three independent experiments (*P < 0.05 by one-way ANOVA followed by Tukey’s multiple comparisons). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33841418), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse ACE‑2 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse ACE‑2 Monoclonal Antibody (Catalog # MAB34371).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse ACE-2 DuoSet ELISA Kit (Catalog # DY3437-05) for convenient development of a sandwich ELISA.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse ACE-2 and eGFP

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse ACE‑2 (Catalog # 3437‑ZN), see our available Western blot detection antibodies

Simple Western

2.5 µg/mL
Sample: Mouse kidney tissue

Western Blot

0.25 µg/mL
Sample: Mouse kidney tissue

Reviewed Applications

Read 3 reviews rated 4.7 using AF3437 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ACE-2

ACE-2, also called ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Mouse ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of mouse somatic ACE. The predicted mouse ACE-2 protein sequence consists of 798 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). In addition, ACE-2 is a key SARS-CoV Spike protein receptor in vivo and has a critical function in acute lung injury (4, 5).

References

  1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  2. Crackower, M.A. et al. (2002) Nature 417:822.
  3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
  4. Kuba, K. et al. (2005) Nature Med. 11:875.
  5. Ima, Y. et al. (2005) Nature 436:112.

Long Name

Angiotensin I Converting Enzyme 2

Alternate Names

ACE2, ACEH

Entrez Gene IDs

59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)

Gene Symbol

ACE2

UniProt

Q8R0I0

Additional ACE-2 Products

Product Documents for Mouse ACE‑2 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse ACE‑2 Antibody

For research use only

Citations for Mouse ACE‑2 Antibody

Customer Reviews for Mouse ACE‑2 Antibody (3)

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Mouse ACE-2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Heart tissue
    Species: Mouse
    Verified Customer | Posted 08/26/2016
    Mouse ACE‑2 Antibody AF3437
  • Mouse ACE-2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adult kidney
    Species: Mouse
    Verified Customer | Posted 06/10/2016
    Mouse ACE‑2 Antibody AF3437
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 22777933
    Species: Mouse
    Verified Customer | Posted 01/07/2015

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