Mouse BMP-4 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB5020-SP
MAB5020-100
Detection of Mouse BMP‑4 by Western Blot.
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Mouse BMP-4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse BMP-4 in direct ELISAs and Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1128D
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived mature mouse BMP-4
Ser293-Arg408
Accession # P21275
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse BMP‑4 by Western Blot. View Larger

Detection of Mouse BMP‑4 by Western Blot. Western blot shows Recombinant Mouse BMP‑4 (Catalog # 5020-BP) and lysates of mouse embryo tissue. PVDF membrane was probed with 0.25 μg/mL of Rabbit Anti-Mouse BMP‑4 Monoclonal Antibody (Catalog # MAB5020) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for BMP‑4 at approximately
40-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry BMP‑4 in Mouse Embryo. View Larger

BMP‑4 in Mouse Embryo. BMP‑4 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Rabbit Anti-Mouse BMP‑4 Monoclonal Antibody (Catalog # MAB5020) at 2 μg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in gastrointestinal tract cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-4

BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1-5). The mouse BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). The propeptide is cleaved intracellularly by furin or proprotein convertase 6, enabling the 15 kDa mature BMP-4 monomer to form an active disulfide linked homodimer or heterodimer with BMP-7 (7-9). Mature mouse and human BMP-4 share 98% aa sequence identity. Mouse BMP-4 shares 85% aa sequence identity with mouse BMP-2 and 35%-54% with other mouse BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR-IA) and type II (primarily Activin RIIA or BMPR-II) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans (13-15).

References
  1. Zhang, P. et al. (2008) Blood 111:1933.
  2. Gambaro, K. et al. (2006) Cell Death Differ. 13:1075.
  3. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
  4. Sadlon, T.J. et al. (2004) Stem Cells 22:457.
  5. Frank, D.B. et al. (2005) Circ. Res. 97:496.
  6. Oida, S. et al. (1995) DNA Seq. 5:273.
  7. Cui, Y. et al. (1998) EMBO J. 17:4735.
  8. Cui, Y. et al. (2001) Genes Dev. 15:2797.
  9. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  10. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
  11. Chen, D. et al. (2004) Growth Factors 22:233.
  12. Lavery, K. et al. (2008) J. Biol. Chem. April 24 epub.
  13. Rosen, V. (2006) Ann. N.Y. Acad. Sci. 1068:19.
  14. Jones, C.M. and J.C. Smith (1998) Dev. Biol. 194:12.
  15. Takada, T. et al. (2003) J. Biol. Chem. 278:43229.
Long Name
Bone Morphogenetic Protein 4
Entrez Gene IDs
652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)
Alternate Names
BMP-2B; BMP2B1; BMP2BMCOPS6; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; OFC11; ZYME

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

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