Mouse CCL17/TARC Antibody

(6 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCL17/TARC in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human TARC is observed. Neutralizes the biological activity of recombinant mouse TARC, and will also neutralize the biological activity of recombinant human TARC using a 20 fold greater Ig concentration.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse CCL17/TARC
    Ala24-Pro93
    Accession # Q9WUZ6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse CCL17/TARC (Catalog # 529-TR)
  • Neutralization
    Measured by its ability to neutralize CCL17/TARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 0.2-0.6 µg/mL in the presence of 0.02 µg/mL Recombinant Mouse CCL17/TARC.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL17/TARC and Neutralization by Mouse CCL17/TARC Antibody.

Recombinant Mouse CCL17/
TARC (Catalog # 529‑TR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL17/TARC (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL17/TARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF529). The ND50 is typically
0.2-0.6 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL17/TARC

Human thymus and activation-regulated chemokine (TARC) also known as CCL17, is a novel CC chemokine identified using a signal sequence trap method. Mouse TARC was discovered as a dendritic cell (DC) specific gene by differentiation RNA display. Mouse TARC cDNA encodes a highly basic 93 amino acid (aa) residue precursor protein with a 23 aa residue putative signal peptide that is cleaved to generate the 70 aa residue mature secreted protein. Among CC chemokine family members, TARC has approximately 24 - 29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3 and I-309. The gene for human TARC has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Mouse TARC is constitutively expressed in thymic DC, and at a lower level in lymph node DC in the lung. Recombinant TARC has been shown to be chemotactic for T cell lines and antigen-primed T helper cells. In humans, TARC was identified to be a specific functional ligand for CCR-4 and CCR-8, receptors that are selectively expressed on T cells.

  • References:
    1. Imai, T. et al. (1997) J. Biol. Chem. 272:15036.
    2. Imai, T. et al. (1996) J. Biol. Chem. 271:21514.
    3. Nomiyama, H. et al. (1997) Genomics 40:211.
    4. Lieberam, I. et al. (1999) Eur. J. Immunol. 29:2684.
  • Entrez Gene IDs:
    6361 (Human); 20295 (Mouse)
  • Alternate Names:
    ABCD-2; CC chemokine TARC; C-C motif chemokine 17; CCL17; chemokine (C-C motif) ligand 17; MGC138273; SCYA17MGC138271; small inducible cytokine subfamily A (Cys-Cys), member 17; Small-inducible cytokine A17; T cell-directed CC chemokine; TARC; TARCA-152E5.3; Thymus and activation-regulated chemokine
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. IL-4/CCL22/CCR4 axis controls regulatory T-cell migration that suppresses inflammatory bone loss in murine experimental periodontitis.
    Authors: Araujo-Pires A, Vieira A, Francisconi C, Biguetti C, Glowacki A, Yoshizawa S, Campanelli A, Trombone A, Sfeir C, Little S, Garlet G
    J Bone Miner Res, 2015;30(3):412-22.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  2. Defective immunoregulation in RSV vaccine-augmented viral lung disease restored by selective chemoattraction of regulatory T cells.
    Authors: Loebbermann, Jens, Durant, Lydia, Thornton, Hannah, Johansson, Cecilia, Openshaw, Peter J
    Proc Natl Acad Sci U S A, 2013;110(8):2987-92.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  3. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells.
    Authors: Jacobsen EA, Ochkur SI, Pero RS, Taranova AG, Protheroe CA, Colbert DC, Lee NA, Lee JJ
    J. Exp. Med., 2008;205(3):699-710.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  5. CCL17 transgenic mice show an enhanced Th2-type response to both allergic and non-allergic stimuli.
    Authors: Tsunemi Y, Saeki H, Nakamura K, Nagakubo D, Nakayama T, Yoshie O, Kagami S, Shimazu K, Kadono T, Sugaya M, Komine M, Matsushima K, Tamaki K
    Eur. J. Immunol., 2006;36(8):2116-27.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. Immunosuppressive effects of CCL17 on pulmonary antifungal responses during pulmonary invasive aspergillosis.
    Authors: Carpenter KJ, Hogaboam CM
    Infect. Immun., 2005;73(11):7198-207.
    Species: Mouse
    Sample Type:
    Application: Neut
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