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Mouse CXCL11/I-TAC Antibody

Catalog #: AF572
5 Citations Datasheet / COA / SDS
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AF572
AF572-SP

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Chemotaxis Induced by CXCL11/I‑TAC and Neutralization by Mouse CXCL11/I‑TAC Antibody.
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Citations (5)
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Mouse CXCL11/I-TAC Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCL11/I-TAC in ELISAs and Western blots. In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant human CXCL11, recombinant mouse (rm) BLC, rmCRG‑2, rmGCP‑2, rmMIG, rmPF4, and rmSDF‑1 alpha is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CXCL11/I-TAC
Phe22-Met100
Accession # Q9JHH5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CXCL11/I-TAC (Catalog # 572-MC)

Mouse CXCL11/I-TAC Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CXCL11/I‑TAC Biotinylated Antibody (Catalog # BAF572)

Standard: Recombinant Mouse CXCL11/I-TAC Protein (Catalog # 572-MC)

Neutralization
Measured by its ability to neutralize CXCL11/I‑TAC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 2-12 µg/mL in the presence of 200 ng/mL Recombinant Mouse CXCL11/I‑TAC.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CXCL11/I‑TAC and Neutralization by Mouse CXCL11/I‑TAC Antibody. View Larger

Chemotaxis Induced by CXCL11/I‑TAC and Neutralization by Mouse CXCL11/I‑TAC Antibody. Recombinant Mouse CXCL11/I-TAC (Catalog # 572-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL11/ I-TAC (200 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL11/I-TAC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF572). The ND50 is typically 2-12 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL11/I-TAC

CXCL11 (also known as I-TAC, SCYB9B, H174, IP-9, and beta -R1) is a member within the non-ELR CXC chemokine subgroup and has been designated CXCL11. CXCL11, together with MIG and IP-10, constitute a subset of chemokines that are ligands for CXCR3, a chemokine receptor that is primarily expressed on activated Th1 cells and NK cells. The three chemokines were also reported to act as antagonists for CCR3, a chemokine receptor that is preferentially expressed on activated Th2 cells. Mouse CXCL11 cDNA encodes a 100 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to yield a 79 aa residue mature protein. Mature mouse and human CXCL11 share 71% aa sequence identity. Mouse CXCL11 also shares 36% and 29% aa sequence identity with mouse IP-10 (CRG-2) and mouse MIG, respectively. The gene for mouse CXCL11 has been mapped to chromosome 5, in close proximity to the IP-10 and MIG genes. Mouse CXCL11 is induced in multiple tissues during endoxemia, with the greatest expression in lung, heart, small intestine, and kidney. The endotoxemia-induced mouse CXCL11 expression is strongly attenuated by treatment with glucocorticoid.

References
  1. Widney, D.P. et al. (2000) J. Immunol. 164:6322.
  2. Meyer, M. et al. (2000) Cytogenet. Cell Genet. 88:278.
  3. Loetscher, P. et al. (2001) J. Biol. Chem. Manuscript M005652200.
Entrez Gene IDs
6373 (Human); 56066 (Mouse)
Alternate Names
beta-R1; b-R1; chemokine (C-X-C motif) ligand 11; CXCL11; H174; H174IP9; Interferon gamma-inducible protein 9; Interferon-inducible T-cell alpha chemoattractant; IP-9member 11; ITAC; I-TAC; I-TACMGC102770; SCYB9B; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; Small-inducible cytokine B11

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Citations for Mouse CXCL11/I-TAC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Identification of CXCL11 as part of chemokine network controlling skeletal muscle development
    Authors: Malte Puchert, Christian Koch, Konstanze Zieger, Jürgen Engele
    Cell and Tissue Research
  2. CXCR7 Antagonism Reduces Acute Lung Injury Pathogenesis
    Authors: L Pouzol, A Sassi, N Baumlin, M Tunis, DS Strasser, F Lehembre, MM Martinic
    Oncogene, 2021-11-05;12(0):748740.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Detection
  3. Complementary Activity of ETV5, RBPJ, and TCF3 Drives Formative Transition from Naive Pluripotency
    Authors: T Kalkan, S Bornelöv, C Mulas, E Diamanti, T Lohoff, M Ralser, S Middelkamp, P Lombard, J Nichols, A Smith
    Cell Stem Cell, 2019-04-25;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Sensing and alarm function of resident memory CD8(+) T cells.
    Authors: Schenkel J, Fraser K, Vezys V, Masopust D
    Nat Immunol, 2013-03-31;14(5):509-13.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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