Mouse Endoglin/CD105 Antibody

(10 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Endoglin in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant rat Endoglin is observed and less than 5% cross-reactivity with recombinant human Endoglin is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Endoglin/CD105
    Glu27-Gly581
    Accession # Q8K100
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    MS-1 mouse cell line
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Endoglin/CD105 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line and
MS‑1 mouse pancreatic islet endothelial cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Endoglin/CD105 at approximately 90-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Endoglin/CD105 in Rat Mesenchymal Stem Cells. Endoglin/CD105 was detected in immersion fixed rat mesenchymal stem cells using Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
Endoglin/CD105 in MS-1 Mouse Cell Line. Endoglin/CD105 was detected in immersion fixed MS-1 mouse pancreatic islet endothelial cell line using Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
Endoglin/CD105 in Mouse Embryo. Endoglin/CD105 was detected in immersion fixed frozen sections of mouse embryo (E13-15) using Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse Endoglin/CD105 by Simple WesternTM. Simple Western lane view shows lysates of bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Endoglin/CD105 at approximately 121 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF1320) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.        
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endoglin/CD105

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% amino acid (aa) identity within the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Mouse Endoglin cDNA encodes 653 aa including a 26 aa signal sequence, a 555 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain (1-3). A mouse isoform with a 35 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The mouse Endoglin ECD shares 69%, 84%, 62%, 63%, and 66% aa identity with human, rat, bovine, porcine, and canine Endoglin, respectively. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFlt-1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

  • References:
    1. Ge, A.Z. and E.C. Butcher (1994) Gene 138:201.
    2. ten Dijke, P. et al. (2008) Angiogenesis 11:79.
    3. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
    4. Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
    5. Moody, J.L. et al. (2007) Stem Cells 25:2809.
    6. Velasco, S. et al. (2008) J. Cell Sci. 121:913.
    7. Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
    8. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
    9. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
    10. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
    11. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
    12. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
    13. Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
    14. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.    
    15. Venkatesha, S. et al. (2006) Nat. Med. 12:642.
  • Entrez Gene IDs:
    2022 (Human); 13805 (Mouse); 497010 (Rat)
  • Alternate Names:
    CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Pharmacologic or Genetic Targeting of Glutamine Synthetase Skews Macrophages toward an M1-like Phenotype and Inhibits Tumor Metastasis
    Authors: EM Palmieri, A Menga, R Martín-Pér, A Quinto, C Riera-Domi, G De Tullio, DC Hooper, WH Lamers, B Ghesquière, DW McVicar, A Guarini, M Mazzone, A Castegna
    Cell Rep, 2017;20(7):1654-1666.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. DNA sequences within glioma-derived extracellular vesicles can cross the intact blood-brain barrier and be detected in peripheral blood of patients
    Authors: N García-Rom, J Carrión-Na, S Esteban-Ru, E Lázaro-Ibá, M Peris-Celd, MM Alonso, J Guzmán-De-, C Fernández-, AO de Mendivi, S García-Duq, C Escobedo-L, R Prat-Acín, C Belda-Inie, A Ayuso-Saci
    Oncotarget, 2017;8(1):1416-1428.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Mast cells decrease efficacy of anti-angiogenic therapy by secreting matrix-degrading granzyme B
    Authors: M Wroblewski, R Bauer, M Cubas Córd, F Udonta, I Ben-Batall, K Legler, C Hauser, J Egberts, M Janning, J Velthaus, C Schulze, K Pantel, C Bokemeyer, S Loges
    Nat Commun, 2017;8(1):269.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. PI3 kinase inhibition improves vascular malformations in mouse models of hereditary haemorrhagic telangiectasia
    Nat Commun, 2016;7(0):13650.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Gene Electrotransfer of Plasmid with Tissue Specific Promoter Encoding shRNA against Endoglin Exerts Antitumor Efficacy against Murine TS/A Tumors by Vascular Targeted Effects.
    Authors: Stimac M, Dolinsek T, Lampreht U, Cemazar M, Sersa G
    PLoS ONE, 2015;10(4):e0124913.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  6. LF-15 & T7, synthetic peptides derived from tumstatin, attenuate aspects of airway remodelling in a murine model of chronic OVA-induced allergic airway disease.
    Authors: Grafton K, Moir L, Black J, Hansbro N, Hansbro P, Burgess J, Oliver B
    PLoS ONE, 2014;9(1):e85655.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  7. Multiple delivery of siRNA against endoglin into murine mammary adenocarcinoma prevents angiogenesis and delays tumor growth.
    Authors: Dolinsek T, Markelc B, Sersa G, Coer A, Stimac M, Lavrencak J, Brozic A, Kranjc S, Cemazar M
    PLoS ONE, 2013;8(3):e58723.
    Species: Human
    Sample Type: Whole Cells
    Application: IF
  8. Focal adhesion kinase regulates the localization and retention of pro-B cells in bone marrow microenvironments.
    Authors: Park S, Wolfram P, Canty K, Harley B, Nombela-Arrieta C, Pivarnik G, Manis J, Beggs H, Silberstein L
    J Immunol, 2013;190(3):1094-102.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-OCT embedded
  9. In vivo adeno-associated viral vector-mediated genetic engineering of white and brown adipose tissue in adult mice.
    Authors: Jimenez V, Munoz S, Casana E, Mallol C, Elias I, Jambrina C, Ribera A, Ferre T, Franckhauser S, Bosch F
    Diabetes, 2013;62(12):4012-22.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  10. Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development.
    Authors: Lin FJ, Chen X, Qin J, Hong YK, Tsai MJ, Tsai SY
    J. Clin. Invest., 2010;120(5):1694-707.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
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Isotype Controls
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Average Rating: 5 (Based on 1 review)

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Mouse Endoglin/CD105 Antibody AF1320
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 ICC/IF Mouse Anonymous 05/16/2017
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Immunocytochemistry/Immunofluorescence Mouse Endoglin/CD105 Antibody AF1320
Immunocytochemistry/Immunofluorescence: Mouse Endoglin/CD105 Antibody [AF1320]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedEmbryonic heart
SpeciesMouse

Other Experimental Details

Other Experimental DetailsBlock - 5% secondary specific serum + 1% BSA + 0.5% Tween-20 - 2hrs at RTPrimary Ab - diluted in block - 1 in 25 - for O/N at 4'CSecondary ab - diluted in block - 2hrs at RT

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