Mouse FGF‑8b Isoform Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse FGF‑8b Isoform in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) FGF acidic, rhFGF basic, rhFGF-4, rhFGF-5, rhFGF-6, rhFGF-7 and rhFGF-9 is observed. It does, however,
    cross‑react with recombinant mouse FGF-8c in neutralizing bioassay.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse FGF-8b
    Gln23-Arg215
    Accession # NP_006110
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse FGF‑8b Isoform (Catalog # 423-F8)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize FGF‑8-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 0.4-2.4 µg/mL in the presence of 60 ng/mL Recombinant Mouse FGF‑8b Isoform and 1 µg/mL heparin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by FGF‑8 and Neutralization by Mouse FGF‑8 Antibody. Recombinant Mouse FGF‑8b Isoform (Catalog # 423-F8) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse FGF‑8b Isoform (60 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse FGF‑8b Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-423-NA). The ND50 is typically 0.4-2.4 µg/mL in the presence of heparin (1 µg/mL).
Immunohistochemistry
FGF‑8 in Human Prostate. FGF‑8 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Mouse FGF‑8 b Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-423-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit  (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial and endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF-8

FGF-8 is a member of the fibroblast growth factor family that was originally discovered as a growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells (1‑3). Alternate splicing of mouse FGF-8 mRNA generates eight secreted isoforms, designated a‑h, but only FGF-8a, b, e and f exist in humans (4). FGF-8 contains a 22 amino acid (aa) signal sequence, an N‑terminal domain that varies according to the isoform (30 aa for FGF-8b; 20 aa for the shortest, FGF-8a), a 125 aa FGF domain and a 37 aa proline‑rich C‑terminal sequence. The FGF domain of FGF-8 shares the most aa identity with FGF17 (75%) and FGF-18 (67%), and the three form an FGF subfamily (2). Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It plays an organizing and inducing role during gastrulation, and regulates patterning of the midbrain/hindbrain, eye, ear, limbs and heart in the embryo (2, 5‑8). The isoforms may play different roles in development. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity although FGF-8a and FGF-8e are also transforming and have been found in human prostate, breast or ovarian tumors (1, 5, 9‑12). FGF-8 shows limited expression in the normal adult, but low levels are found in the reproductive and genitourinary tract, peripheral leukocytes and bone marrow hematopoietic cells (3, 9, 13).

  • References:
    1. Mattila, M.M. and P.L. Harkonen (2007) Cytokine Growth Factor Rev. 18:257.
    2. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.
    3. Tanaka, A. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8928.
    4. Gemel, J. et al. (1996) Genomics 35:253.
    5. Olsen, S.K. et al. (2006) Genes Dev. 20:185.
    6. Crossley, P.H. et al. (1996) Cell, 84:127.
    7. Heikinheimo, M. et al. (1994) Mech. Dev. 48:129.
    8. Sun, X. et al. (1999) Genes Dev. 13:1834.
    9. Ghosh, A.K. et al. (1996) Cell Growth Differ. 7:1425.
    10. Mattila, M.M. et al. (2001) Oncogene 20:2791.
    11. Valve, E. et al. (2000) Int. J. Cancer 88:718.
    12. Valve, E.M. et al. (2001) Lab. Invest. 81:815.
    13. Nezu, M. et al. (2005) Biochem. Biophys. Res. Commun. 335:843.
  • Long Name:
    Fibroblast Growth Factor 8
  • Entrez Gene IDs:
    2253 (Human); 14179 (Mouse); 29349 (Rat)
  • Alternate Names:
    AIGF; AIGFKAL6; Androgen-induced growth factor; FGF8; FGF-8; fibroblast growth factor 8 (androgen-induced); fibroblast growth factor 8; HBGF-8; Heparin-binding growth factor 8; MGC149376
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Fgf20 is required for sensory epithelial specification in the developing cochlea.
    Authors: Hayashi T, Ray CA, Bermingham-McDonogh O
    J. Neurosci., 2008;28(23):5991-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  2. Cardiac arterial pole alignment is sensitive to FGF8 signaling in the pharynx.
    Authors: Hutson MR, Zhang P, Stadt HA, Sato AK, Li YX, Burch J, Creazzo TL, Kirby ML
    Dev. Biol., 2006;295(2):486-97.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: Neut
  3. FGF8 is required for cell survival at distinct stages of nephrogenesis and for regulation of gene expression in nascent nephrons.
    Authors: Grieshammer U, Cebrian C, Ilagan R, Meyers E, Herzlinger D, Martin GR
    Development, 2005;132(17):3847-57.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Neut
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