Mouse FGF-8 b Isoform Antibody

R&D Systems | Catalog # AF-423-NA

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Avian - Chicken

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FGF-8 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse FGF-8b
Gln23-Arg215
Accession # NP_006110

Specificity

Detects mouse FGF‑8b Isoform in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) FGF acidic, rhFGF basic, rhFGF-4, rhFGF-5, rhFGF-6, rhFGF-7 and rhFGF-9 is observed. It does, however,cross‑react with recombinant mouse FGF-8c in neutralizing bioassay.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse FGF-8 b Isoform Antibody

Cell Proliferation Induced by FGF‑8 and Neutralization by Mouse FGF‑8 Antibody.

Cell Proliferation Induced by FGF‑8 and Neutralization by Mouse FGF‑8 Antibody.

Recombinant Mouse FGF-8b Isoform (Catalog # 423-F8) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse FGF-8b Isoform (60 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse FGF-8b Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-423-NA). The ND50 is typically 0.4-2.4 µg/mL in the presence of heparin (1 µg/mL).
FGF-8 antibody in Human Prostate by Immunohistochemistry (IHC-P).

FGF‑8 in Human Prostate.

FGF-8 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Mouse FGF-8 b Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-423-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial and endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Mouse FGF-8 b Isoform Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse FGF‑8b Isoform (Catalog # 423-F8)

Neutralization

Measured by its ability to neutralize FGF‑8-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 0.4-2.4 µg/mL in the presence of 60 ng/mL Recombinant Mouse FGF‑8b Isoform and 1 µg/mL heparin.

Reviewed Applications

Read 1 review rated 5 using AF-423-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-8

FGF-8 is a member of the fibroblast growth factor family that was originally discovered as a growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells (1‑3). Alternate splicing of mouse FGF-8 mRNA generates eight secreted isoforms, designated a‑h, but only FGF-8a, b, e and f exist in humans (4). FGF-8 contains a 22 amino acid (aa) signal sequence, an N‑terminal domain that varies according to the isoform (30 aa for FGF-8b; 20 aa for the shortest, FGF-8a), a 125 aa FGF domain and a 37 aa proline‑rich C‑terminal sequence. The FGF domain of FGF-8 shares the most aa identity with FGF17 (75%) and FGF-18 (67%), and the three form an FGF subfamily (2). Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It plays an organizing and inducing role during gastrulation, and regulates patterning of the midbrain/hindbrain, eye, ear, limbs and heart in the embryo (2, 5‑8). The isoforms may play different roles in development. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity although FGF-8a and FGF-8e are also transforming and have been found in human prostate, breast or ovarian tumors (1, 5, 9‑12). FGF-8 shows limited expression in the normal adult, but low levels are found in the reproductive and genitourinary tract, peripheral leukocytes and bone marrow hematopoietic cells (3, 9, 13).

References

  1. Mattila, M.M. and P.L. Harkonen (2007) Cytokine Growth Factor Rev. 18:257.
  2. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.
  3. Tanaka, A. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8928.
  4. Gemel, J. et al. (1996) Genomics 35:253.
  5. Olsen, S.K. et al. (2006) Genes Dev. 20:185.
  6. Crossley, P.H. et al. (1996) Cell, 84:127.
  7. Heikinheimo, M. et al. (1994) Mech. Dev. 48:129.
  8. Sun, X. et al. (1999) Genes Dev. 13:1834.
  9. Ghosh, A.K. et al. (1996) Cell Growth Differ. 7:1425.
  10. Mattila, M.M. et al. (2001) Oncogene 20:2791.
  11. Valve, E. et al. (2000) Int. J. Cancer 88:718.
  12. Valve, E.M. et al. (2001) Lab. Invest. 81:815.
  13. Nezu, M. et al. (2005) Biochem. Biophys. Res. Commun. 335:843.

Long Name

Fibroblast Growth Factor 8

Alternate Names

AIGF, FGF8, HBGF-8

Entrez Gene IDs

2253 (Human); 14179 (Mouse); 29349 (Rat)

Gene Symbol

FGF8

UniProt

NP_006110

Additional FGF-8 Products

Product Documents for Mouse FGF-8 b Isoform Antibody

Certificate of Analysis

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Product Specific Notices for Mouse FGF-8 b Isoform Antibody

For research use only

Citations for Mouse FGF-8 b Isoform Antibody

Customer Reviews for Mouse FGF-8 b Isoform Antibody (1)

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  • Mouse FGF‑8b Isoform Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: E11.5 mouse embryo fixed in 4% PFA
    Species: Mouse
    Verified Customer | Posted 02/12/2021
    Dilution used - 1:50. The staining was done on an E11.5 mouse transverse section (fixed on 4% PFA overnight) and done using standard IF techniques. Blocking - 1 hour with 1% BSA before addition of the primary antibody Secondary antibody - Alexa Fluor Donkey Anti Goat 488
    Mouse FGF-8 b Isoform Antibody AF-423-NA

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