Mouse Frizzled-4 Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Frizzled-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) Frizzled-8 and less than 2% cross-reactivity with rmFrizzled-3 and rmFrizzled-7 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4
    Phe37-Glu180
    Accession # Q8BLL2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Frizzled‑4 Fc Chimera (Catalog # 194-FZ)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Frizzled‑4 in Embryonic Mouse Neuronal Tissue. Frizzled‑4 was detected in immersion fixed frozen sections of embryonic mouse neuronal tissue (E11) using 15 µg/mL Goat Anti-Mouse Frizzled‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF194) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane and cytoplasm of neuronal cells in the midbrain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Frizzled-4

Frizzled-4, designated CD344, is a 7-transmembrane glycoprotein of the Frizzled family within the G-protein coupled receptor superfamily (1, 2). Frizzled proteins function as receptors for Wnt proteins and can activate canonical Wnt/beta-catenin signaling as well as planar cell polarity and calcium flux pathways (1). Frizzled-4 is particularly important in angiogenic Wnt pathway signaling (1, 5). Frizzleds contain a divergent N-terminal signal peptide, a highly conserved ~120  amino acid (aa) cysteine-rich domain (CRD), a variable length linker region, seven transmembrane domains, and a variable-length C-terminal tail (1). The mouse Frizzled-4 cDNA encodes 537 aa with a 36 aa signal sequence and a 186 aa N-terminal extracellular sequence (4). The portion expressed includes aa 37-180, and shares 93%, 94%, 90%, 89%, and 88% identity with human, rat, equine, bovine and canine Frizzled-4, respectively. This portion competes for Wnt binding with endogenous receptors. In humans, a 122 aa soluble form that diverges at aa 95 is proposed to be a positive regulator of Wnt signaling pathways (5). Frizzled-4 is unusual in binding a non-wnt ligand, Norrin, in addition to binding Wnt ligands (1, 3, 6). Norrin binds the Frizzled-4 CRD, activates Wnt signaling pathways and uses LRP5/6 as co-receptors (3, 6). Deletion of either Frizzled-4 or Norrin in mice results in a similar phenotype including malformation of vasculature in the retina, cerebellar degeneration, and loss of hair cells in the inner ear (1, 3, 7). In humans, blindness due to familial exudative vitreoretinopathy (FEVR) is associated with mutations producing loss of function of Frizzled-4 or Norrin, designated EVR1 and EVR2, respectively (1, 3, 8). Frizzled-4 expression has been found in many tissues, including mouse ovary, where it influences corpus luteum vasculogenesis and is necessary for fertility (4, 9).

  • References:
    1. Huang, H-C. and P.S. Klein (2004) Genome Biol. 5:234.
    2. Parmalee, N.L. and J. Kitajewski (2008) Curr. Drug Targets 9:558.
    3. Xu, Q. et al. (2004) Cell 116:883.
    4. Wang, Y. et al. (1996) J. Biol. Chem. 271:4468.
    5. Sagara, N. et al. (2001) Biochem. Biophys. Res. Commun. 282:750.
    6. Smallwood, P.M. et al. (2007) J. Biol. Chem. 282:4057.
    7. Wang, Y. et al. (2001) J. Neurosci. 21:4761.
    8. Robitaille, J. et al. (2002) Nat. Genet. 32:326.
    9. Hsieh, M. et al. (2005) Biol. Reprod. 73:1135.
  • Entrez Gene IDs:
    8322 (Human); 14366 (Mouse)
  • Alternate Names:
    CD344 antigen; CD344; EVR1; exudative vitreoretinopathy 1; FEVR; frizzled (Drosophila) homolog 4; frizzled homolog 4 (Drosophila); Frizzled4; Frizzled-4; Fz-4; FZD4; FZD4S; FzE4; GPCR; hFz4; MGC34390; WNT receptor frizzled-4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Amyloid-beta binds to the extracellular cysteine-rich domain of Frizzled and inhibits Wnt/beta-catenin signaling.
    Authors: Magdesian MH, Carvalho MM, Mendes FA, Saraiva LM, Juliano MA, Juliano L, Garcia-Abreu J, Ferreira ST
    J. Biol. Chem., 2008;283(14):9359-68.
    Species: Mouse
    Sample Type: Recombinant Protein
    Application: ELISA Development
  2. MicroRNAs regulate ocular neovascularization.
    Authors: Shen J, Yang X, Xie B, Chen Y, Swaim M, Hackett SF, Campochiaro PA
    Mol. Ther., 2008;16(7):1208-16.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Wnt5a signaling induces proliferation and survival of endothelial cells in vitro and expression of MMP-1 and Tie-2.
    Authors: Masckauchan TN, Agalliu D, Vorontchikhina M, Ahn A, Parmalee NL, Li CM, Khoo A, Tycko B, Brown AM, Kitajewski J
    Mol. Biol. Cell, 2006;17(12):5163-72.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
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