Mouse Glypican 2 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Glypican 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human (rh) rhGlypican 2 is observed and less than 1% cross-reactivity with rhGlypican 3, recombinant mouse Glypican 5, and rhGlypican 6 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Glypican 2
    Lys27-Leu554
    Accession # Q8BKV1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Glypican 2 (Catalog # 2355-GP)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Glypican 2 in Mouse Embryo. Glypican 2 was detected in perfusion fixed frozen sections of mouse embryo (11 d.p.c.) using Goat Anti-Mouse Glypican 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2355) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal root ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glypican 2

The glypicans (GPC) constitute a family of heparan sulfate proteoglycans that are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1‑GPC6). All glypican core proteins contain an N-terminal signal peptide, a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. Based on the degree of their amino acid sequence similarity, two subfamilies of glypicans have been defined. One subfamily (sharing from 35‑63% sequence homology) includes GPC1, 2, 4, and 6, while the second subfamily (sharing 54% sequence identity) includes GPC3 and 5. Proteins between the two subfamilies also share 17‑25% sequence similarity (1‑4). Glypicans are widely expressed in adult and fetal tissues. During embryonic development, the expression level of the various glypicans changes in a stage and tissue specific manner. GPC2, also known as cerebroglycan, is primarily expressed in developing neuronal tissues including the brain, spinal cord, dorsal root ganglia, and cranial nerves. It is found on the tracts of actively growing axons (5). Cell surface GPC2 binds midkine, indicating midkine‑GPC2 interaction may participate in neuronal cell migration and neurite outgrowth (6).

  • References:
    1. Filmus, J. and S.B. Selleck (2001) J. Clin. Invest. 108:497.
    2. De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117.
    3. Song, H. and J. Filmus (2002) Biochem. Biophys. Acta 1573:241.
    4. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
    5. Ivins, J.K. et al. (1997) Dev. Biol. 184:320.
    6. Kurosawa, N. et al. (2001) Glycoconj. J. 18:499.
  • Entrez Gene IDs:
    221914 (Human); 71951 (Mouse)
  • Alternate Names:
    Cerebroglycan; Glypican 2; GPC2
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