Mouse ICAM-2/CD102 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse ICAM-2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human ICAM‑2 and recombinant rat ICAM-1 and less than 1% cross-reactivity with recombinant mouse (rm) VCAM-1, rmICAM-5, and rmICAM-1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse ICAM-2
    Ser20-Gln222
    Accession # P35330
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Adhesion Blockade
    The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Mouse ICAM-2/CD102 Fc Chimera (Catalog # 774-M2, 12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse ICAM‑2/CD102 by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ICAM‑2/CD102 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF774) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for ICAM‑2/CD102 at approximately 50 to 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
ICAM‑2/CD102 in Mouse Ovary. ICAM‑2/CD102 was detected in perfusion fixed frozen sections of mouse ovary using 15 µg/mL Goat Anti-Mouse ICAM‑2/CD102 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF774) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-2/CD102

Intercellular Adhesion Molecule-2 (ICAM-2, also known as CD102), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). ICAM-2 is constitutively expressed at high levels on vascular endothelial cells and lymphohematopoietic cells. ICAM-2 mediated adhesion has been shown to provide a co-stimulatory signal for T cell aggregation, NK cytotoxicity and NK cell migration. The mouse and human ICAM-2 proteins share 60% amino acid identity. Human LFA-1 binds equally well to human or mouse ICAM-2.

  • References:
    1. Somersalo, K. et al. (1995) J. Biol. Chem. 270:8629.
    2. Hayflick, J. et al. (1998) Immunologic Res. 17:313.
    3. Xu, H. (1992) J. Immunol. 149:2650.
  • Long Name:
    Intercellular Adhesion Molecule 2
  • Entrez Gene IDs:
    3384 (Human); 15896 (Mouse)
  • Alternate Names:
    CD102 antigen; CD102; ICAM2; ICAM-2; intercellular adhesion molecule 2
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