Mouse ICAM-2/CD102 Antibody Summary
Accession # P35330
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse ICAM‑2/CD102 by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ICAM-2/CD102 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF774) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for ICAM-2/CD102 at approximately 50 to 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ICAM‑2/CD102 in Mouse Ovary. ICAM‑2/CD102 was detected in perfusion fixed frozen sections of mouse ovary using 15 µg/mL Goat Anti-Mouse ICAM‑2/CD102 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF774) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Intercellular Adhesion Molecule-2 (ICAM-2, also known as CD102), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). ICAM-2 is constitutively expressed at high levels on vascular endothelial cells and lymphohematopoietic cells. ICAM-2 mediated adhesion has been shown to provide a co-stimulatory signal for T cell aggregation, NK cytotoxicity and NK cell migration. The mouse and human ICAM-2 proteins share 60% amino acid identity. Human LFA-1 binds equally well to human or mouse ICAM-2.
- Somersalo, K. et al. (1995) J. Biol. Chem. 270:8629.
- Hayflick, J. et al. (1998) Immunologic Res. 17:313.
- Xu, H. (1992) J. Immunol. 149:2650.
Citation for Mouse ICAM-2/CD102 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Leukocyte-endothelial cell interaction is enhanced in podocalyxin-deficient mice
Authors: G Porras, MS Ayuso, C González-M
Int. J. Biochem. Cell Biol., 2018;99(0):72-79.
Sample Types: Cell Lysates
Applications: Western Blot
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