Mouse IFITM3/Fragilis Antibody

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Detection of Mouse IFITM3/Fragilis by Western Blot.
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Product Details
Citations (5)
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Mouse IFITM3/Fragilis Antibody Summary

Species Reactivity
Detects mouse IFITM3/Fragilis in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human IFITM3 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant mouse IFITM3/Fragilis
Accession # Q9CQW9
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IFITM3/Fragilis antibody by Western Blot. View Larger

Detection of Mouse IFITM3/Fragilis by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, D3 mouse embryonic stem cell line, and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IFITM3/Fragilis Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IFITM3/Fragilis at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry IFITM3/Fragilis antibody in Embryonic Mouse Ovary by Immunohistochemistry (IHC-Fr). View Larger

IFITM3/Fragilis in Embryonic Mouse Ovary. IFITM3/Fragilis was detected in immersion fixed frozen sections of embryonic mouse ovary (E13.5) using 10 µg/mL Goat Anti-Mouse IFITM3/Fragilis Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3377) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFITM3/Fragilis

Mouse IFITM3/CD225 (also Fragilis) is a 137 amino acids (aa) 2-transmembrane protein that belongs to the Fragilis family of interferon-inducible genes. It contains two terminal extracellular domains (aa 1-57 and 131-137) and one cytoplasmic region that potentially allows for multiple phosphorylations. The N-terminal extracellular region of mouse IFITM3 shares 54%, 91%, and 58% amino acid sequence identity to the equivalent region in bovine, rat and human IFITM3, respectively. IFITM3 seems to be involved in primordial germ cell clustering and regionalization.

Long Name
Interferon-induced Transmembrane Protein 3
Entrez Gene IDs
10410 (Human); 66141 (Mouse)
Alternate Names
1-8U; Fragilis; IFITM3; interferon induced transmembrane protein 3 (1-8U); interferon-induced transmembrane protein 3; Interferon-inducible protein 1-8U; IP15; mil-1

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Citations for Mouse IFITM3/Fragilis Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. OTX2 restricts entry to the mouse germline
    Authors: J Zhang, M Zhang, D Acampora, M Vojtek, D Yuan, A Simeone, I Chambers
    Nature, 2018-10-03;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. Influenza virus differentially activates mTORC1 and mTORC2 signaling to maximize late stage replication
    Authors: SK Kuss-Duerk, J Wang, I Mena, K White, G Metreveli, R Sakthivel, MA Mata, R Muñoz-More, X Chen, F Krammer, MS Diamond, ZJ Chen, A García-Sas, BMA Fontoura
    PLoS Pathog., 2017-09-27;13(9):e1006635.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. IFITM1 suppresses expression of human endogenous retroviruses in human embryonic stem cells
    Authors: Y Fu, Z Zhou, H Wang, P Gong, R Guo, J Wang, X Lu, F Qi, L Liu
    FEBS Open Bio, 2017-06-29;7(8):1102-1110.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  4. Interferon-induced transmembrane protein 3 is a type II transmembrane protein.
    Authors: Bailey, Charles, Kondur, Hema R, Huang, I-Chueh, Farzan, Michael
    J Biol Chem, 2013-09-25;288(45):32184-93.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Ifitm3 limits the severity of acute influenza in mice.
    Authors: Bailey, Charles, Huang, I-Chueh, Kam, Christin, Farzan, Michael
    PLoS Pathog, 2012-09-06;8(9):e1002909.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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