|IFITM3/Fragilis in Embryonic Mouse Ovary. IFITM3/Fragilis was detected in immersion fixed frozen sections of embryonic mouse ovary (E13.5) using 10 µg/mL Goat Anti-Mouse IFITM3/Fragilis Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3377) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.|
|Detection of Mouse IFITM3/Fragilis by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, D3 mouse embryonic stem cell line, and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IFITM3/Fragilis Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IFITM3/Fragilis at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
Mouse IFITM3/CD225 (also Fragilis) is a 137 amino acids (aa) 2-transmembrane protein that belongs to the Fragilis family of interferon-inducible genes. It contains two terminal extracellular domains (aa 1-57 and 131-137) and one cytoplasmic region that potentially allows for multiple phosphorylations. The N-terminal extracellular region of mouse IFITM3 shares 54%, 91%, and 58% amino acid sequence identity to the equivalent region in bovine, rat and human IFITM3, respectively. IFITM3 seems to be involved in primordial germ cell clustering and regionalization.
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