Mouse IFITM3/Fragilis Antibody

Catalog #: AF3377
5 Citations Datasheet / COA / SDS

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AF3377
AF3377-SP

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Detection of Mouse IFITM3/Fragilis by Western Blot.

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Product Details

Citations (5)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IFITM3/Fragilis Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IFITM3/Fragilis in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human IFITM3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse IFITM3/Fragilis
Asn2-His57
Accession # Q9CQW9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse IFITM3/Fragilis by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, D3 mouse embryonic stem cell line, and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IFITM3/Fragilis Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IFITM3/Fragilis at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

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IFITM3/Fragilis in Embryonic Mouse Ovary. IFITM3/Fragilis was detected in immersion fixed frozen sections of embryonic mouse ovary (E13.5) using 10 µg/mL Goat Anti-Mouse IFITM3/Fragilis Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3377) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFITM3/Fragilis

Mouse IFITM3/CD225 (also Fragilis) is a 137 amino acids (aa) 2-transmembrane protein that belongs to the Fragilis family of interferon-inducible genes. It contains two terminal extracellular domains (aa 1-57 and 131-137) and one cytoplasmic region that potentially allows for multiple phosphorylations. The N-terminal extracellular region of mouse IFITM3 shares 54%, 91%, and 58% amino acid sequence identity to the equivalent region in bovine, rat and human IFITM3, respectively. IFITM3 seems to be involved in primordial germ cell clustering and regionalization.

Long Name

Interferon-induced Transmembrane Protein 3

Entrez Gene IDs

10410 (Human); 66141 (Mouse)

Alternate Names

1-8U; Fragilis; IFITM3; interferon induced transmembrane protein 3 (1-8U); interferon-induced transmembrane protein 3; Interferon-inducible protein 1-8U; IP15; mil-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Primordial and Mature Germ Cells

Citations for Mouse IFITM3/Fragilis Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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OTX2 restricts entry to the mouse germline
Authors: J Zhang, M Zhang, D Acampora, M Vojtek, D Yuan, A Simeone, I Chambers
Nature, 2018-10-03;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Influenza virus differentially activates mTORC1 and mTORC2 signaling to maximize late stage replication
Authors: SK Kuss-Duerk, J Wang, I Mena, K White, G Metreveli, R Sakthivel, MA Mata, R Muñoz-More, X Chen, F Krammer, MS Diamond, ZJ Chen, A García-Sas, BMA Fontoura
PLoS Pathog., 2017-09-27;13(9):e1006635.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
IFITM1 suppresses expression of human endogenous retroviruses in human embryonic stem cells
Authors: Y Fu, Z Zhou, H Wang, P Gong, R Guo, J Wang, X Lu, F Qi, L Liu
FEBS Open Bio, 2017-06-29;7(8):1102-1110.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
Interferon-induced transmembrane protein 3 is a type II transmembrane protein.
Authors: Bailey, Charles, Kondur, Hema R, Huang, I-Chueh, Farzan, Michael
J Biol Chem, 2013-09-25;288(45):32184-93.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Ifitm3 limits the severity of acute influenza in mice.
Authors: Bailey, Charles, Huang, I-Chueh, Kam, Christin, Farzan, Michael
PLoS Pathog, 2012-09-06;8(9):e1002909.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot