Mouse IL-13 Antibody

(6 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-13 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant rat IL‑13 is observed, and approximately 10% cross-reactivity with recombinant human IL‑13 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse IL-13
    Ser26-Phe131
    Accession # P20109
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse IL‑13 (Catalog # 413-ML)
  • Immunocytochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.05-0.15 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑13.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody. Recombinant Mouse IL‑13 (Catalog # 413-ML) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA). The ND50 is typically 0.05‑0.15 µg/mL.

Immunocytochemistry

IL‑13 in Mouse Splenocytes. IL‑13 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-13

IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature mouse IL-13 shares 57%, 75%, and 58% amino acid sequence identity with human, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

  • References:
    1. Wills-Karp, M. (2004) Immunol. Rev. 202:175.
    2. Nakajima, H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
    3. Brown, K.D. et al. (1989) J. Immunol. 142:679.
    4. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
    5. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
    6. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
    7. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
    8. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
    9. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
    10. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
    11. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
    12. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387.
    13. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464.
    14. Tabata, Y. et al. (2007) J. Immunol. 177:7905.
    15. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
    16. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
    17. Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.
  • Long Name:
    Interleukin 13
  • Entrez Gene IDs:
    3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)
  • Alternate Names:
    ALRHMGC116789; BHR1interleukin-13; IL13; IL-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Smoking p66Shc knocked out mice develop respiratory bronchiolitis with fibrosis but not emphysema.
    Authors: Lunghi B, De Cunto G, Cavarra E, Fineschi S, Bartalesi B, Lungarella G, Lucattelli M
    PLoS ONE, 2015;10(3):e0119797.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  2. Small interfering RNA against transcription factor STAT6 inhibits allergic airway inflammation and hyperreactivity in mice.
    Authors: Darcan-Nicolaisen Y, Meinicke H, Fels G, Hegend O, Haberland A, Kuhl A, Loddenkemper C, Witzenrath M, Kube S, Henke W, Hamelmann E
    J. Immunol., 2009;182(12):7501-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor alpha2 in vivo.
    Authors: Chen W, Tabata Y, Gibson AM, Daines MO, Warrier MR, Wills-Karp M, Hershey GK
    J. Allergy Clin. Immunol., 2008;122(3):625-32.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  4. The IL-4/IL-13/Stat6 signalling pathway promotes luminal mammary epithelial cell development.
    Authors: Khaled WT, Read EK, Nicholson SE, Baxter FO, Brennan AJ, Came PJ, Sprigg N, McKenzie AN, Watson CJ
    Development, 2007;134(15):2739-50.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  5. Modulation of airway remodeling and airway inflammation by peroxisome proliferator-activated receptor gamma in a murine model of toluene diisocyanate-induced asthma.
    Authors: Lee KS, Park SJ, Kim SR, Min KH, Jin SM, Lee HK, Lee YC
    J. Immunol., 2006;177(8):5248-57.
    Species: Mouse
    Sample Type: BALF
    Application: WB
  6. Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling.
    Authors: Daines MO, Tabata Y, Walker BA, Chen W, Warrier MR, Basu S, Hershey GK
    J. Immunol., 2006;176(12):7495-501.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
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