Mouse IL-13 Antibody

Catalog # Availability Size / Price Qty
AF-413-NA
AF-413-SP
Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody.
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Product Details
Citations (13)
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Mouse IL-13 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-13 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL-13
Ser26-Phe131
Accession # P20109
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑13 (Catalog # 413-ML)
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.05-0.15 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑13.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody. View Larger

Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody. Recombinant Mouse IL-13 (413-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA). The ND50 is typically 0.05-0.15 µg/mL.

Immunocytochemistry IL-13 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

IL‑13 in Mouse Splenocytes. IL-13 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse IL-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry Detection of Mouse IL-13 by Immunohistochemistry View Larger

Detection of Mouse IL-13 by Immunohistochemistry Histological lung sections of a p66Shc−/− mouse at 7 months after CS exposure.(A) Positive immunostaining for MAC-3 confirms that the free alveolar cells are predominantly macrophages. A small number of macrophages are also present in the peribronchiolar infiltrates. (B) Representative lung section showing alveolar multinucleated macrophages containing fine granular brown cytoplasmic particles that stain with Perls’ Prussian blue (C). (D) The peribronchiolar areas of inflammation are characterized by a large amount of lymphocytes and a small number of pigmented cells that also stain with Perl’s Prussian blue (E). (F) Many fascin positive cells (histiocytes) are present in the peribronchiolar areas. (A) and (F): Scale bars = 80 μm; (B-E): Scale bars = 40 μm. Immunohistochemistry of CD4 (G), IL-4 (H), IL-13 (I), Arginase I (J), Chitinase (K) and iNOS (L) in the lung tissue of p66Shc−/− mice at 7 months after CS exposure. In Fig. 4L, a M1 macrophage (iNOS positive)(arrowhead) is present together iNOS negative macrophages. (G-L): Scale bars = 40 μm. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0119797), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-13

IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature mouse IL-13 shares 57%, 75%, and 58% amino acid sequence identity with human, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

References
  1. Wills-Karp, M. (2004) Immunol. Rev. 202:175.
  2. Nakajima, H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
  3. Brown, K.D. et al. (1989) J. Immunol. 142:679.
  4. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
  5. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
  6. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
  7. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
  8. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
  9. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
  10. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
  11. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
  12. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387.
  13. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464.
  14. Tabata, Y. et al. (2007) J. Immunol. 177:7905.
  15. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
  16. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
  17. Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.
Long Name
Interleukin 13
Entrez Gene IDs
3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)
Alternate Names
ALRHMGC116789; BHR1interleukin-13; IL13; IL-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600

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Citations for Mouse IL-13 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. CD8+ T cell-derived IL-13 increases macrophage IL-10 to resolve neuropathic pain
    Authors: SK Singh, K Krukowski, GO Laumet, D Weis, JF Alexander, CJ Heijnen, A Kavelaars
    JCI Insight, 2022-03-08;7(5):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay, In Vivo
  2. Dual vaccination against IL-4 and IL-13 protects against chronic allergic asthma in mice
    Authors: E Conde, R Bertrand, B Balbino, J Bonnefoy, J Stackowicz, N Caillot, F Colaone, S Hamdi, R Houmadi, A Loste, JBJ Kamphuis, F Huetz, L Guillemina, N Gaudenzio, A Mougel, D Hardy, JN Snouwaert, BH Koller, V Serra, P Bruhns, G Grouard-Vo, LL Reber
    Nature Communications, 2021-05-11;12(1):2574.
    Species: Mouse
    Sample Types: Recombinant Proteins
    Applications: Western Blot
  3. Group 2 Innate Lymphoid Cells Must Partner with the Myeloid-Macrophage Lineage for Long-Term Postviral Lung Disease
    Authors: K Wu, X Wang, SP Keeler, BJ Gerovac, EV Agapov, DE Byers, S Gilfillan, M Colonna, Y Zhang, MJ Holtzman
    J. Immunol., 2020-07-08;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Lack of interleukin-13 receptor ?1 delays the loss of dopaminergic neurons during chronic stress
    Authors: S Mori, S Sugama, W Nguyen, T Michel, MG Sanna, M Sanchez-Al, R Cintron-Co, G Moroncini, Y Kakinuma, P Maher, B Conti
    J Neuroinflammation, 2017-04-21;14(1):88.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Smoking p66Shc knocked out mice develop respiratory bronchiolitis with fibrosis but not emphysema.
    Authors: Lunghi B, De Cunto G, Cavarra E, Fineschi S, Bartalesi B, Lungarella G, Lucattelli M
    PLoS ONE, 2015-03-19;10(3):e0119797.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Neutrophils prime a long-lived effector macrophage phenotype that mediates accelerated helminth expulsion.
    Authors: Chen, Fei, Wu, Wenhui, Millman, Ariel, Craft, Joshua F, Chen, Eunice, Patel, Nirav, Boucher, Jean L, Urban, Joseph F, Kim, Charles, Gause, William
    Nat Immunol, 2014-08-31;15(10):938-46.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  7. Diesel exhaust particles induce cysteine oxidation and s-glutathionylation in house dust mite induced murine asthma.
    Authors: Lee, Gerald B, Brandt, Eric B, Xiao, Chang, Gibson, Aaron M, Le Cras, Timothy, Brown, Lou Ann, Fitzpatrick, Anne M, Khurana Hershey, Gurjit K
    PLoS ONE, 2013-03-29;8(3):e60632.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development (Capture)
  8. Macrophages as IL-25/IL-33-responsive cells play an important role in the induction of type 2 immunity.
    Authors: Yang Z, Grinchuk V, Urban J, Bohl J, Sun R, Notari L, Yan S, Ramalingam T, Keegan A, Wynn T, Shea-Donohue T, Zhao A
    2013-03-25;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  9. Small interfering RNA against transcription factor STAT6 inhibits allergic airway inflammation and hyperreactivity in mice.
    Authors: Darcan-Nicolaisen Y, Meinicke H, Fels G, Hegend O, Haberland A, Kuhl A, Loddenkemper C, Witzenrath M, Kube S, Henke W, Hamelmann E
    J. Immunol., 2009-06-15;182(12):7501-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor alpha2 in vivo.
    Authors: Chen W, Tabata Y, Gibson AM, Daines MO, Warrier MR, Wills-Karp M, Hershey GK
    J. Allergy Clin. Immunol., 2008-08-09;122(3):625-32.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  11. The IL-4/IL-13/Stat6 signalling pathway promotes luminal mammary epithelial cell development.
    Authors: Khaled WT, Read EK, Nicholson SE, Baxter FO, Brennan AJ, Came PJ, Sprigg N, McKenzie AN, Watson CJ
    Development, 2007-07-04;134(15):2739-50.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  12. Modulation of airway remodeling and airway inflammation by peroxisome proliferator-activated receptor gamma in a murine model of toluene diisocyanate-induced asthma.
    Authors: Lee KS, Park SJ, Kim SR, Min KH, Jin SM, Lee HK, Lee YC
    J. Immunol., 2006-10-15;177(8):5248-57.
    Species: Mouse
    Sample Types: BALF
    Applications: Western Blot
  13. Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling.
    Authors: Daines MO, Tabata Y, Walker BA, Chen W, Warrier MR, Basu S, Hershey GK
    J. Immunol., 2006-06-15;176(12):7495-501.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Mouse IL-13 Antibody
By Katie Myers on 11/29/2022
Application: ELISA Sample Tested: Serum Species: Mouse

Worked as ELISA detection