Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, ELISA Development, ELISA Development (Capture), In vivo assay, Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-13 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL-13
Ser26-Phe131
Accession # P20109

Specificity

Detects mouse IL-13 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse IL‑13 Antibody

Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody.

Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody.

Recombinant Mouse IL-13 (413-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA). The ND50 is typically 0.05-0.15 µg/mL.

IL-13 antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

IL‑13 in Mouse Splenocytes.

IL-13 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse IL-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Mouse IL-13 by Immunohistochemistry

Detection of Mouse IL-13 by Immunohistochemistry

Histological lung sections of a p66Shc−/− mouse at 7 months after CS exposure.(A) Positive immunostaining for MAC-3 confirms that the free alveolar cells are predominantly macrophages. A small number of macrophages are also present in the peribronchiolar infiltrates. (B) Representative lung section showing alveolar multinucleated macrophages containing fine granular brown cytoplasmic particles that stain with Perls’ Prussian blue (C). (D) The peribronchiolar areas of inflammation are characterized by a large amount of lymphocytes and a small number of pigmented cells that also stain with Perl’s Prussian blue (E). (F) Many fascin positive cells (histiocytes) are present in the peribronchiolar areas. (A) and (F): Scale bars = 80 μm; (B-E): Scale bars = 40 μm. Immunohistochemistry of CD4 (G), IL-4 (H), IL-13 (I), Arginase I (J), Chitinase (K) and iNOS (L) in the lung tissue of p66Shc−/− mice at 7 months after CS exposure. In Fig. 4L, a M1 macrophage (iNOS positive)(arrowhead) is present together iNOS negative macrophages. (G-L): Scale bars = 40 μm. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0119797), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse IL-13 by Immunohistochemistry

Detection of Mouse IL-13 by Immunohistochemistry

Histological lung sections of a p66Shc−/− mouse at 7 months after CS exposure.(A) Positive immunostaining for MAC-3 confirms that the free alveolar cells are predominantly macrophages. A small number of macrophages are also present in the peribronchiolar infiltrates. (B) Representative lung section showing alveolar multinucleated macrophages containing fine granular brown cytoplasmic particles that stain with Perls’ Prussian blue (C). (D) The peribronchiolar areas of inflammation are characterized by a large amount of lymphocytes and a small number of pigmented cells that also stain with Perl’s Prussian blue (E). (F) Many fascin positive cells (histiocytes) are present in the peribronchiolar areas. (A) and (F): Scale bars = 80 μm; (B-E): Scale bars = 40 μm. Immunohistochemistry of CD4 (G), IL-4 (H), IL-13 (I), Arginase I (J), Chitinase (K) and iNOS (L) in the lung tissue of p66Shc−/− mice at 7 months after CS exposure. In Fig. 4L, a M1 macrophage (iNOS positive)(arrowhead) is present together iNOS negative macrophages. (G-L): Scale bars = 40 μm. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0119797), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse IL‑13 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IL‑13 (Catalog # 413-ML)

Neutralization

Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.05-0.15 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑13.

Reviewed Applications

Read 1 review rated 5 using AF-413-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-13

IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature mouse IL-13 shares 57%, 75%, and 58% amino acid sequence identity with human, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

References

  1. Wills-Karp, M. (2004) Immunol. Rev. 202:175.
  2. Nakajima, H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
  3. Brown, K.D. et al. (1989) J. Immunol. 142:679.
  4. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
  5. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
  6. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
  7. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
  8. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
  9. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
  10. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
  11. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
  12. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387.
  13. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464.
  14. Tabata, Y. et al. (2007) J. Immunol. 177:7905.
  15. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
  16. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
  17. Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.

Long Name

Interleukin 13

Alternate Names

IL13, NC30, P600

Entrez Gene IDs

3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)

Gene Symbol

IL13

UniProt

P20109

Additional IL-13 Products

Product Documents for Mouse IL‑13 Antibody

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Product Specific Notices for Mouse IL‑13 Antibody

For research use only

Citations for Mouse IL‑13 Antibody

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  • Mouse IL-13 Antibody
    Name: Katie Myers
    Application: ELISA
    Sample Tested: Serum
    Species: Mouse
    Verified Customer | Posted 11/29/2022
    Worked as ELISA detection
    Mouse IL‑13 Antibody AF-413-NA

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