Mouse IL-17D Antibody

Catalog # Availability Size / Price Qty
AF2274
AF2274-SP
Mouse IL‑17D ELISA Standard Curve.
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Mouse IL-17D Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-17D in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human (rh) IL-17D is observed and less than 1% cross-reactivity with recombinant mouse (rm) IL-17, rmIL-17B, rmIL-17C, rmIL‑17E, and rmIL-17F is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL‑17D
Ala25-Arg205
Accession # Q8K4C4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑17D (Catalog # 2274-ML)
ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse IL‑17D Monoclonal Antibody (Catalog # MAB22741).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse IL-17D DuoSet ELISA Kit (Catalog # DY2274) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Mouse IL‑17D ELISA Standard Curve. Recombinant Mouse IL‑17D protein was serially diluted 2-fold and captured by Rat Anti-Mouse IL‑17D Monoclonal Antibody (MAB22741) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Mouse IL‑17D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2274) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17D

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2, 6). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2, 6). Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members, have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL‑17 RE, have also been reported (1‑6). Mouse IL-17D is synthesized as a 205 amino acid (aa) precursor that contains a putative 24 aa signal peptide and a 181 aa mature segment. The mature region contains two potential N-linked glycosylation sites and eight cysteines, four of which are involved in the formation of a modified cysteine-knot motif (5). The molecule is reported to exist as a 53 kDa disulfide-linked homodimer (2, 5). Given that its predicted homodimeric molecular weight is 40 kDa, the molecule is presumably glycosylated. In the mature region, mouse IL-17D is 88% aa identical to human IL-17D. There is less than 30% aa identity between mouse IL-17D and other members of the mouse IL-17 family. IL-17D is expressed in skeletal muscle, adipose tissue, fetal liver, and heart, plus resting CD4+ T cells and CD19+ B cells (1). R&D Systems has shown IL-17D binding to a mouse IL-17 R/Fc construct in a functional ELISA. IL-17D is known to induce the production of IL-8, IL-6 and GM-CSF (5).

References
  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
  4. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
  5. Starnes, T. et al. (2002) J. Immunol. 169:642.
  6. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
Long Name
Interleukin 17D
Entrez Gene IDs
53342 (Human); 239114 (Mouse)
Alternate Names
FLJ30846; IL17D; IL-17D; IL-17Dinterleukin 27; IL-22; IL-27interleukin-17D; IL27interleukin-27; interleukin 17D; Interleukin-27

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Mouse IL-17D Antibody
By Anonymous on 06/02/2016
Application: IP Sample Tested: Cancer cell lysates Species: Mouse