Mouse IL-21 R Antibody AF596: R&D Systems

Mouse IL-21 R Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-21 R in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant human IL‑21 R is observed, and less than 1% cross-reactivity will recombinant mouse IL-2 R beta is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-21 R
    Leu21-Pro236
    Accession # Q9JHX3
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse IL‑21 R Subunit Fc Chimera (Catalog # 596-MR)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    Immersion fixed mouse splenocytes
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑21 R in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Goat Anti-Mouse IL‑21 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF596, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-21 R

The interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 R, also called NILR (novel interleukin receptor) is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 R is a 529 amino acid (aa) residue protein with a 19 aa signal peptide, a 217 aa extracellular domain, an 18 aa transmembrane domain, and a 275 aa cytoplasmic domain. Mouse and human IL-21 R share 62% aa identity. IL-21 R is expressed on lymphoid tissues, peripheral B cells, and cell lines of T, B and natural killer lineage. Although not fully elucidated, the IL-2 R gamma ( gamma c) chain appears to play a role in IL-21 R signaling. The IL-21/IL-21 R interaction appears to play important roles in B and T cell proliferation after antigen stimulation and natural killer cell maturation.

  • References:
    1. Parrish-Novak, J. et al. (2000) Nature 408:57.
    2. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
    3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
    4. Asao, H. et al. (2001) J. Immunol. 167:1.
  • Long Name:
    Interleukin 21 Receptor
  • Entrez Gene IDs:
    50615 (Human); 60504 (Mouse)
  • Alternate Names:
    CD360 antigen; CD360; IL-21 R; IL-21 receptor; IL21R; IL-21R; interleukin 21 receptor; interleukin-21 receptor; MGC10967; NILR; Novel interleukin receptor
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