Mouse IL-22 Antibody (140301)

R&D Systems | Catalog # MAB582

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 140301
View all IL-22 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL-22
Leu34-Val179
Accession # Q9JJY9

Specificity

Detects mouse IL-22 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant rat IL-22 is observed, 15% cross-reactivity with recombinant human IL-22 is observed, and no cross-reactivity with recombinant mouse IL-10 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse IL-22 Antibody (140301)

Detection of IL-22 antibody in Mouse Th17 Splenocytes antibody by Flow Cytometry.

Detection of IL‑22 in Mouse Th17 Splenocytes by Flow Cytometry.

Mouse splenocytes differentiated to Th17 cells with plate-bound Rat anti-Mouse CD3e monoclonal antibody (MAB484, 5 µg/mL) plus Goat anti-Mouse CD28 (AF483, 5 µg/mL), Recombinant Human TGF-beta 1 (240-B, 10 ng/mL) Recombinant Mouse IL-23 (1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (MAB485, 10 µg/mL) for 5 days then re-stimulated with PMA (50 ng/ml), Ca2+ Ionomycin (200 ng/ml) and Brefeldin A ( 5 μg/ml) for 4 hours, were stained with APC-conjugated Rat anti-Mouse CD4 Monoclonal Antibody (FAB554A) and (A) Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB582) or (B) isotype control antibody (MAB006) followed by PE-conjugated Goat anti-Rat secondary antibody (F0105B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (FC012). View our protocol for Staining Intracellular Molecules.

Applications for Mouse IL-22 Antibody (140301)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: Mouse splenocytes treated with plate-bound Rat anti-Mouse CD3 epsilon  monoclonal antibody (Catalog # MAB484, 5 μg/ml) plus Goat anti-Mouse CD28 (Catalog # AF483, 5 μg/ml), Recombinant Human TGF-beta 1 (Catalog # 240-B, 10 ng/ml) Recombinant Mouse IL-23 (Catalog # 1887-ML, 20 ng/ml), Recombinant Mouse IL-6 (Catalog # 406-ML, 40 ng/ml), Recombinant Mouse IL-1 beta (Catalog # 401-ML, 10 ng/ml), and Rat anti-Mouse IFN-gamma (Catalog # MAB485, 10 μg/ml) for 5 days,  then re-stimulated with PMA (50 ng/ml), Ca2+ Ionomycin (200 ng/ml) and Brefeldin A ( 5 μg/ml) for 4 hours, were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (Catalog # FC012).

Western Blot

1 µg/mL
Sample: Recombinant Mouse IL‑22 (Catalog # 582-ML)

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-22

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. The mouse IL-22 gene is localized to chromosome 10. Although it exists as a single copy gene in many mouse strains, the IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

References

  1. Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
  2. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Dumoutier, L. et al. (2000) PNAS 97:10144.
  4. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.

Long Name

Interleukin 22

Alternate Names

IL-TIF, IL22

Entrez Gene IDs

50616 (Human); 50929 (Mouse); 500836 (Rat)

Gene Symbol

IL22

UniProt

Q9JJY9

Additional IL-22 Products

Product Documents for Mouse IL-22 Antibody (140301)

Certificate of Analysis

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Product Specific Notices for Mouse IL-22 Antibody (140301)

For research use only

Citations for Mouse IL-22 Antibody (140301)

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