Mouse IL-7 Antibody

Catalog # Availability Size / Price Qty
AF407
AF407-SP
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Cell Proliferation Induced by IL‑7 and Neutralization by Mouse IL‑7 Antibody.
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Product Details
Citations (7)
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Reviews (1)

Mouse IL-7 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-7 in ELISAs and Western blots. In sandwich immunoassays, approximately 20% cross-reactivity with recombinant rat IL-7 is observed, and less than 0.1% cross‑reactivity with recombinant human IL-7 is observed. Neutralizes the biological activity of recombinant mouse IL-7. It will not neutralize the biological activity of recombinant human IL-7.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL-7
Glu26-Ile154
Accession # Q544C8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑7 (Catalog # 407-ML)

Mouse IL-7 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IL‑7 Biotinylated Antibody (Catalog # BAF407)

Standard: Recombinant Mouse IL-7 Protein (Catalog # 407-ML)

Neutralization
Measured by its ability to neutralize IL‑7-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC). The Neutralization Dose (ND50) is typically 0.2-0.4 µg/mL in the presence of 1.5 ng/mL Recombinant Mouse IL‑7.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑7 and Neutralization by Mouse IL‑7 Antibody. View Larger

Cell Proliferation Induced by IL‑7 and Neutralization by Mouse IL‑7 Antibody. Recombinant Mouse IL-7 (Catalog # 407-ML) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-7 (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF407). The ND50 is typically 0.2-0.4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-7

IL-7, previously known as pre-B-cell growth factor and lymphopoietin-1, was originally purified on the basis of its ability to promote the proliferation of precursor B‑cells. It has now been shown that IL-7 can also stimulate the proliferation of thymocytes, T cell progenitors and mature CD4 + and CD8+ T cells. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells as well as the development of cytotoxic T lymphocytes (CTL). IL-7 was also shown to induce the V(D)J rearrangement of the T cell receptor beta gene in mouse fetal thymocytes. Among myeloid lineage cells, IL-7 can up-regulate the production of pro-inflammatory cytokines and stimulate the tumorocidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues.

Mouse IL-7 cDNA encodes a precursor protein of 154 amino residues containing a 25 amino acid residue signal peptide. Human IL-7 has approximately 65% amino acid sequence identity with mouse IL-7 and both proteins exhibit cross-species activity.

IL-7 bioactivities are mediated by the binding of IL-7 to functional high-affinity receptor complexes. The ligand binding subunit (IL-7 R) of the IL-7 receptor complex has been cloned from human and mouse sources. In addition to the membrane-anchored form of the IL-7 receptor, a human cDNA clone that encodes a soluble form of the IL-7 R has also been isolated. The gamma  chain of the IL-2 receptor complex has been shown to be an essential component for IL-7 signal transduction. Both IL-7 R and IL-2 R gamma are members of the hematopoietin receptor superfamily. Cells known to express IL-7 receptors include pre-B cells, T cells and bone marrow cells.

Long Name
Interleukin 7
Entrez Gene IDs
3574 (Human); 16196 (Mouse); 25647 (Rat)
Alternate Names
IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF

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Citations for Mouse IL-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Blockade of IL-7 signaling suppresses inflammatory responses and reverses alopecia areata in C3H/HeJ mice
    Authors: Z Dai, EHC Wang, L Petukhova, Y Chang, EY Lee, AM Christiano
    Science Advances, 2021-04-02;7(14):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Commensal microflora and interferon-gamma promote steady-state interleukin-7 production in vivo.
    Authors: Shalapour S, Deiser K, Sercan O, Tuckermann J, Minnich K, Willimsky G, Blankenstein T, Hammerling GJ, Arnold B, Schuler T
    Eur. J. Immunol., 2010-09-01;40(9):2391-400.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. BCL6 is critical for the development of a diverse primary B cell repertoire.
    Authors: Duy C, Yu JJ, Nahar R, Swaminathan S, Kweon SM, Polo JM, Valls E, Klemm L, Shojaee S, Cerchietti L, Schuh W, Jack HM, Hurtz C, Ramezani-Rad P, Herzog S, Jumaa H, Koeffler HP, de Alboran IM, Melnick AM, Ye BH, Muschen M
    J. Exp. Med., 2010-05-24;207(6):1209-21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Thymic regulation of autoimmune disease by accelerated differentiation of Foxp3+ regulatory T cells through IL-7 signaling pathway.
    Authors: Chen X, Fang L, Song S, Guo TB, Liu A, Zhang JZ
    J. Immunol., 2009-10-19;183(10):6135-44.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  5. Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells.
    Authors: Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG
    Blood, 2007-01-16;109(9):3706-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells.
    Authors: Johnson SE, Shah N, Panoskaltsis-Mortari A, Lebien TW
    J. Immunol., 2005-12-01;175(11):7325-31.
    Species: Mouse
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: ELISA Development, Neutralization
  7. A recombinant single-chain IL-7/HGFbeta hybrid cytokine induces juxtacrine interactions of the IL-7 and HGF (c-Met) receptors and stimulates the proliferation of CFU-S12, CLPs, and pre-pro-B cells.
    Authors: Lai L, Zeff RA, Goldschneider I
    Blood, 2005-11-22;107(5):1776-84.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Mouse IL‑7 Antibody
By Katie Myers on 11/14/2022
Application: ELISA Sample Tested: Serum Species: Mouse

Worked well as a detection in our Mouse ELISA.