Mouse IL-9 Antibody

Catalog # Availability Size / Price Qty
AB-409-NA
Cell Proliferation Induced by IL‑9 and Neutralization by Mouse IL‑9 Antibody.
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Mouse IL-9 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL‑9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human IL-9 is observed. Neutralizes the biological activity of recombinant mouse IL-9. It will not neutralize the biological activity of recombinant human IL-9.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑9
Gln19-Pro144
Accession # P15247
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IL‑9 (Catalog # 409-ML)
Neutralization
Measured by its ability to neutralize IL‑9-induced proliferation in the TS1 mouse helper T cell line. Uyttenhove, C. et al. (1988) Proc. Natl. Acad. Sci. 85:6934. The Neutralization Dose (ND50) is typically 3-18 µg/mL in the presence of 0.05 ng/mL Recombinant Mouse IL‑9.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by IL‑9 and Neutralization by Mouse IL‑9 Antibody. View Larger

Cell Proliferation Induced by IL‑9 and Neutralization by Mouse IL‑9 Antibody. Recombinant Mouse IL‑9 (Catalog # 409-ML) stimulates proliferation in the TS1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑9 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑9 Polyclonal Antibody (Catalog # AB-409-NA). The ND50 is typically 3-18 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-9

Mouse IL-9 was originally identified as a T cell-derived T cell growth factor III/P40 which could support the long term growth of certain mouse T helper clones in the absence of antigen or antigen-presenting cells. IL-9 can also prolong the in vitro survival of other T cell clones as well as potentiate the IL-2 dependent proliferation of mouse fetal thymocytes. However, this cytokine has no growth-stimulating activity on mouse cytolytic T cell clones or fresh T cells. In addition to its activities on T cells, mouse IL-9 also has mast cell enhancing activity (MEA) and can enhance the mIL-3- or mIL-4-dependent proliferation of mouse bone marrow-derived mast cells. Furthermore, IL-9 will synergize with erythropoietin to support erythroid colony formation in vitro.

The gene for mIL-9 has been mapped to mouse chromosome 13. The mouse IL-9 cDNA encodes a 144 amino acid residue precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a predicted molecular mass of 14 kDa. Mouse IL-9 contains four potential N-linked glycosylation sites and the native mIL-9 is a highly glycosylated protein.

Human IL-9 was independently cloned as a novel growth factor which is mitogenic for the human megakaryoblastic leukemic cell line, M07e. Human and mouse IL-9 share 56% and 67% homology at the amino acid and nucleotide levels, respectively. Although mouse IL-9 is active on human cells, human IL-9 is not active on mouse cells. 

References
  1. Renauld, J.E. et al. (1995) J. Leukoc. Biol 57:303.
Long Name
Interleukin 9
Entrez Gene IDs
3578 (Human); 16198 (Mouse)
Alternate Names
Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40

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Isotype Controls

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