Mouse Oncostatin M/OSM Antibody

(7 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse OSM in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human OSM is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse OSM
    Ala24-Arg206
    Accession # P53347
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Oncostatin M/OSM (Catalog # 495-MO)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize Oncostatin M/OSM-induced proliferation in the NIH‑3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.6-3.0 µg/mL in the presence of 15 ng/mL Recombinant Mouse Oncostatin M/OSM.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Oncostatin M/OSM in Mouse Embryo. Oncostatin M/OSM was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c, section through spinal cord) using Mouse Oncostatin M/OSM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-495-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
Oncostatin M/OSM in Mouse Embryo. Oncostatin M/OSM was detected in immersion fixed frozen sections of mouse embryo using Mouse Oncostatin M/OSM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-495-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Mouse Oncostatin M/OSM Antibody. Recombinant Mouse Oncostatin M/OSM (Catalog # 495-MO) stimulates proliferation in the NIH‑3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Oncostatin M/OSM (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Oncostatin M/OSM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-495-NA). The ND50 is typically 0.6-3.0 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Oncostatin M/OSM

Oncostatin M (OSM) is a member of a cytokine subfamily that includes IL-6, IL-11, LIF, CNTF, and cardiotrophin-1. These cytokines have overlapping biological functions and shared receptor components. Mouse OSM was cloned and identified as an immediate early gene induced in various myeloid and lymphoid cell lines by a subset of cytokines including IL-2, IL-3, GM-CSF, and EPO. The mouse OSM cDNA encodes a 263 amino acid residue precursor protein that shows 48% identity with human OSM. Similar to human OSM, the C-terminal region of mouse OSM contains a highly charged region. Deletion of this C-terminal region appears to be essential for the formation of biologically active mOSM.

The biological activity of human OSM has been shown to be mediated either by the LIF/OSM receptor complex composed of gp130 and LIF R alpha or by a human OSM specific receptor composed of gp130 and OSM R alpha. It remains to be determined if the biological activities of mouse OSM can also be mediated by both receptor complexes in mouse cells.

  • References:
    1. Yoshimura, A. et al. (1996) The EMBO Journal 15:1055.
    2. Ray, P. et al. (1996) Endocrinology 137:1151.
    3. Rose, T.M. and A.G. Bruce (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, editor, Oxford University Press, New York, p. 127.
  • Entrez Gene IDs:
    5008 (Human); 18413 (Mouse)
  • Alternate Names:
    MGC20461; oncostatin M; oncostatin-M; OSM
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Anti-OSM Antibody Inhibits Tubulointerstitial Lesion in a Murine Model of Lupus Nephritis
    Authors: Q Liu, Y Du, K Li, W Zhang, X Feng, J Hao, H Li, S Liu
    Mediators Inflamm., 2017;2017(0):3038514.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  2. Oncostatin M overexpression induces skin inflammation but is not required in the mouse model of imiquimod-induced psoriasis-like inflammation
    Eur J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  3. Oncostatin m, an inflammatory cytokine produced by macrophages, supports intramembranous bone healing in a mouse model of tibia injury.
    Authors: Guihard P, Boutet M, Brounais-Le Royer B, Gamblin A, Amiaud J, Renaud A, Berreur M, Redini F, Heymann D, Layrolle P, Blanchard F
    Am J Pathol, 2015;185(3):765-75.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  4. Activation of NFAT signaling establishes a tumorigenic microenvironment through cell autonomous and non-cell autonomous mechanisms.
    Authors: Tripathi, P, Wang, Y, Coussens, M, Manda, K R, Casey, A M, Lin, C, Poyo, E, Pfeifer, J D, Basappa, N, Bates, C M, Ma, L, Zhang, H, Pan, M, Ding, L, Chen, F
    Oncogene, 2014;33(14):1840-9.
  5. Oncostatin M acting via OSMR, augments the actions of IL-1 and TNF in synovial fibroblasts.
    Authors: Le Goff B, Singbrant S, Tonkin B, Martin T, Romas E, Sims N, Walsh N
    Cytokine, 2014;68(2):101-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  6. Long term oncostatin M treatment induces an osteocyte-like differentiation on osteosarcoma and calvaria cells.
    Authors: Brounais B, David E, Chipoy C, Trichet V, Ferre V, Charrier C, Duplomb L, Berreur M, Redini F, Heymann D, Blanchard F
    Bone, 2009;44(5):830-9.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: Neut
  7. Cardiotrophin-1 in choroid plexus and the cerebrospinal fluid circulatory system.
    Authors: Gard AL, Gavin E, Solodushko V, Pennica D
    Neuroscience, 2004;127(1):43-52.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
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