Mouse P-Cadherin Antibody

Catalog # Availability Size / Price Qty
AF761
AF761-SP
Detection  of  P‑Cadherin by Western  Blot.
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Product Details
Citations (4)
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Mouse P-Cadherin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects P‑Cadherin in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross-reactivity with recombinant human (rh) P‑Cadherin is observed and less than 0.3% cross-reactivity with recombinant mouse E-Cadherin, rhN-Cadherin, and rhCadherin-8 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse P-Cadherin
Glu100-Gly647
Accession # Q8BSL6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
25 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
XB2 mouse teratoma keratinocyte cell line
Immunohistochemistry
5-15 µg/mL
See below
Adhesion Blockade
The adhesion of A431 human epithelial carcinoma cells (1 x 105 cells/well) to immobilized Recombinant Mouse P-Cadherin Fc Chimera (Catalog # 761-MP, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 50 µg/mL of the antibody.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Mouse P-Cadherin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse P‑Cadherin Biotinylated Antibody (Catalog # BAF761)

Standard: Recombinant Mouse P-Cadherin Fc Chimera Protein, CF (Catalog # 761-MP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of  P‑Cadherin by Western  Blot. View Larger

Detection of P‑Cadherin by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse
P‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for P‑Cadherin at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry P‑Cadherin  in A431 Human Cell Line. View Larger

P‑Cadherin in A431 Human Cell Line.
P‑Cadherin was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Mouse P‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to intercellular junctions. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry P‑Cadherin in Mouse Embryo. View Larger

P‑Cadherin in Mouse Embryo. P‑Cadherin was detected in immersion fixed frozen sections of mouse embryo (15 dpc) using Goat Anti-Mouse
P‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue and lungs. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection  of Mouse  P‑Cadherin by  Simple WesternTM. View Larger

Detection of Mouse P‑Cadherin by Simple WesternTM. Simple Western lane view shows lysates of mouse embryo tissue, loaded at 0.2 mg/mL. A specific band was detected for P‑Cadherin at approximately 115 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse P‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P-Cadherin

Placental Cadherin (P-Cadherin or PCAD) is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Constitutive P-Cadherin expression is found in the epidermis, mesothelium, corneal epithelium, and uterine decidua. Mouse P-Cadherin is an 822 amino acid (aa) protein with a 27 aa signal sequence and a 795 aa propeptide. The mature protein begins at aa 100 and has a 542 aa extracellular region, a 27 aa transmembrane region, and a 153 aa cytoplasmic region.

References
  1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  2. Overduin, M. et al. (1995) Science 267:386.
  3. Takeichi, M. (1991) Science 251:1451.
  4. Nose, A. et al. (1987) EMBO J. 6:3655.
Long Name
Placental Cadherin
Entrez Gene IDs
1001 (Human); 12560 (Mouse); 116777 (Rat)
Alternate Names
CAD3; cadherin 3, P-cadherin (placental); cadherin 3, type 1, P-cadherin (placental); Cadherin-3; CDH3; CDHP; CDHPcalcium-dependent adhesion protein, placental; HJMD; PCAD; PCadherin; P-Cadherin; PCADP-cadherin; Placental cadherin

Product Datasheets

Citations for Mouse P-Cadherin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Cadherins in the retinal pigment epithelium (RPE) revisited: P-cadherin is the highly dominant cadherin expressed in human and mouse RPE in vivo
    Authors: X Yang, JY Chung, U Rai, N Esumi
    PLoS ONE, 2018;13(1):e0191279.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Stem cell plasticity enables hair regeneration following Lgr5(+) cell loss
    Authors: JD Hoeck, B Biehs, AV Kurtova, NM Kljavin, F de Sousa E, B Alicke, H Koeppen, Z Modrusan, R Piskol, FJ de Sauvage
    Nat. Cell Biol., 2017;19(6):666-676.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Stem Cell Lineage Infidelity Drives Wound Repair and Cancer
    Authors: Y Ge, NC Gomez, RC Adam, M Nikolova, H Yang, A Verma, CP Lu, L Polak, S Yuan, O Elemento, E Fuchs
    Cell, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Role of cell and matrix-bound VEGF isoforms in lens development.
    Authors: Saint-Geniez M, Kurihara T, D'Amore PA
    Invest. Ophthalmol. Vis. Sci., 2009;50(1):311-21.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen

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