Mouse P-Cadherin Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects P‑Cadherin in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross-reactivity with recombinant human (rh) P‑Cadherin is observed and less than 0.3% cross-reactivity with recombinant mouse E-Cadherin, rhN-Cadherin, and rhCadherin-8 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse P-Cadherin
    Glu100-Gly647
    Accession # Q8BSL6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    25 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    XB2 mouse teratoma keratinocyte cell line
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Adhesion Blockade
    The adhesion of A431 human epithelial carcinoma cells (1 x 105 cells/well) to immobilized Recombinant Mouse P-Cadherin Fc Chimera (Catalog # 761-MP, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 50 µg/mL of the antibody.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
    • Mouse P-Cadherin Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse P‑Cadherin Antibody (Catalog # AF761)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse P‑Cadherin Biotinylated Antibody (Catalog # BAF761)
  • ELISA Standard
     
    Recombinant Mouse P-Cadherin Fc Chimera Protein, CF (Catalog # 761-MP)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of P‑Cadherin by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse
P‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for P‑Cadherin at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
P‑Cadherin in A431 Human Cell Line.
P‑Cadherin was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Mouse P‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to intercellular junctions. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
P‑Cadherin in Mouse Embryo. P‑Cadherin was detected in immersion fixed frozen sections of mouse embryo (15 dpc) using Goat Anti-Mouse
P‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue and lungs. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse P‑Cadherin by Simple WesternTM. Simple Western lane view shows lysates of mouse embryo tissue, loaded at 0.2 mg/mL. A specific band was detected for P‑Cadherin at approximately 115 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse P‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: P-Cadherin

Placental Cadherin (P-Cadherin or PCAD) is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Constitutive P-Cadherin expression is found in the epidermis, mesothelium, corneal epithelium, and uterine decidua. Mouse P-Cadherin is an 822 amino acid (aa) protein with a 27 aa signal sequence and a 795 aa propeptide. The mature protein begins at aa 100 and has a 542 aa extracellular region, a 27 aa transmembrane region, and a 153 aa cytoplasmic region.

  • References:
    1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
    2. Overduin, M. et al. (1995) Science 267:386.
    3. Takeichi, M. (1991) Science 251:1451.
    4. Nose, A. et al. (1987) EMBO J. 6:3655.
  • Long Name:
    Placental Cadherin
  • Entrez Gene IDs:
    1001 (Human); 12560 (Mouse); 116777 (Rat)
  • Alternate Names:
    CAD3; cadherin 3, P-cadherin (placental); cadherin 3, type 1, P-cadherin (placental); Cadherin-3; CDH3; CDHP; CDHPcalcium-dependent adhesion protein, placental; HJMD; PCAD; PCadherin; P-Cadherin; PCADP-cadherin; Placental cadherin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Stem cell plasticity enables hair regeneration following Lgr5(+) cell loss
    Authors: JD Hoeck, B Biehs, AV Kurtova, NM Kljavin, F de Sousa E, B Alicke, H Koeppen, Z Modrusan, R Piskol, FJ de Sauvage
    Nat. Cell Biol., 2017;19(6):666-676.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Role of cell and matrix-bound VEGF isoforms in lens development.
    Authors: Saint-Geniez M, Kurihara T, D'Amore PA
    Invest. Ophthalmol. Vis. Sci., 2009;50(1):311-21.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

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Mounting Medium

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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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