Detection of Mouse Pref‑1/DLK1/FA1 by Western Blot.
Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose-like cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Pref‑1/DLK1/FA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8277) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pref‑1/DLK1/FA1 at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Pref‑1/DLK1/FA1 in 3T3‑L1 Mouse Cell Line by Flow Cytometry.
3T3‑L1 mouse embryonic fibroblast adipose-like cell line was stained with Goat Anti-Mouse Pref‑1/DLK1/FA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8277, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Pref‑1/DLK1/FA1 in Mouse Embryo.
Pref‑1/DLK1/FA1 was detected in immersion fixed frozen sections of mouse embryonic lung using Goat Anti-Mouse Pref‑1/DLK1/FA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8277) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Pref-1 (Preadipocyte factor 1, Protein delta homolog 1, DLK1, FA1 and Fetal antigen 1) is a 45-60 kDa transmembrane glycoprotein that is highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary. Expression of Pref-1 is elevated in liver after birth but starts to decline around postnatal day 16. It contains 6 EGF-like domains and is involved in embryonic skeletal system development. Pref-1 inhibits preadipocyte proliferation by regulating their entry into G1/S-phase and represses preadipocyte differentiation. It is a master regulator of preadipocyte homeostasis and adipose tissue expansion. Pref-1 manipulation may, therefore, be utilized in obesity treatments. Mouse Pref-1 shares 81% aa identity with human Pref-1.
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