Mouse Pref-1/DLK1/FA1 Antibody

Catalog # Availability Size / Price Qty
AF8277
AF8277-SP

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Detection of Mouse Pref‑1/DLK1/FA1 by Western Blot.
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Product Details
Citations (4)
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Mouse Pref-1/DLK1/FA1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Pref‑1/DLK1/FA1 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant human Pref-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Pref-1/DLK1/FA1
Ala24-Gln305
Accession # Q09163
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Pref-1/DLK1/FA1 antibody by Western Blot. View Larger

Detection of Mouse Pref‑1/DLK1/FA1 by Western Blot. Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Pref-1/DLK1/FA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8277) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pref-1/DLK1/FA1 at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of Pref-1/DLK1/FA1 antibody in 3T3-L1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Pref‑1/DLK1/FA1 in 3T3‑L1 Mouse Cell Line by Flow Cytometry. 3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Goat Anti-Mouse Pref-1/DLK1/FA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8277, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Immunohistochemistry Pref-1/DLK1/FA1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Pref‑1/DLK1/FA1 in Mouse Embryo. Pref-1/DLK1/FA1 was detected in immersion fixed frozen sections of mouse embryonic lung using Goat Anti-Mouse Pref-1/DLK1/FA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8277) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pref-1/DLK1/FA1

Pref-1 (Preadipocyte factor 1, Protein delta homolog 1, DLK1, FA1 and Fetal antigen 1) is a 45-60 kDa transmembrane glycoprotein that is highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary. Expression of Pref-1 is elevated in liver after birth but starts to decline around postnatal day 16. It contains 6 EGF-like domains and is involved in embryonic skeletal system development. Pref-1 inhibits preadipocyte proliferation by regulating their entry into G1/S-phase and represses preadipocyte differentiation. It is a master regulator of preadipocyte homeostasis and adipose tissue expansion. Pref-1 manipulation may, therefore, be utilized in obesity treatments. Mouse Pref-1 shares 81% aa identity with human Pref-1.

Long Name
Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1
Entrez Gene IDs
8788 (Human); 13386 (Mouse)
Alternate Names
delta-like 1 homolog (Drosophila); DLK; DLK1; DLK-1; FA1; FA1fetal antigen 1; pG2; pG2delta-like homolog (Drosophila); preadipocyte factor 1; Pref1; Pref-1; protein delta homolog 1; secredeltin; ZOG

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Citations for Mouse Pref-1/DLK1/FA1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration
    Authors: Sun, L;Zhang, X;Wu, S;Liu, Y;Guerrero-Juarez, CF;Liu, W;Huang, J;Yao, Q;Yin, M;Li, J;Ramos, R;Liao, Y;Wu, R;Xia, T;Zhang, X;Yang, Y;Li, F;Heng, S;Zhang, W;Yang, M;Tzeng, CM;Ji, C;Plikus, MV;Gallo, RL;Zhang, LJ;
    Cell reports
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Increased prostaglandin-D2 in male STAT3-deficient hearts shifts cardiac progenitor cells from endothelial to white adipocyte differentiation
    Authors: E Stelling, M Ricke-Hoch, S Erschow, S Hoffmann, AK Bergmann, M Heimerl, S Pietzsch, K Battmer, A Haase, B Stapel, M Scherr, JL Balligand, O Binah, D Hilfiker-K
    PloS Biology, 2020-12-28;18(12):e3000739.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Obesity induces preadipocyte CD36 expression promoting inflammation via the disruption of lysosomal calcium homeostasis and lysosome function
    Authors: X Luo, Y Li, P Yang, Y Chen, L Wei, T Yu, J Xia, XZ Ruan, L Zhao, Y Chen
    EBioMedicine, 2020-06-06;56(0):102797.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Mesenchymal Precursor Cells in Adult Nerves Contribute to Mammalian Tissue Repair and Regeneration
    Authors: MJ Carr, JS Toma, APW Johnston, PE Steadman, SA Yuzwa, N Mahmud, PW Frankland, DR Kaplan, FD Miller
    Cell Stem Cell, 2018-11-29;0(0):.
    Species: Mouse
    Sample Types:

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