Mouse/Rat Cathepsin C/DPPI Antibody

(8 citations)
(1 Review)
  
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse Cathepsin C/DPPI in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human (rh) Cathepsin C is observed and less than 1% cross-reactivity with recombinant mouse (rm) Cathepsin A, rmCathepsin B, rmCathepsin D, rmCathepsin H, and rmCathepsin X/Z/P is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin C/DPPI
    Asp25-Leu462
    Accession # P97821
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25-1 µg/mL
    See below
  • Simple Western
    2.5 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Cathepsin C/DPPI by Western Blot. Western blot shows lysates of mouse liver tissue and mouse spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cathepsin C/DPPI at approximately 20-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse and Rat Cathepsin C/DPPI by Western Blot. Western blot shows lysates of mouse lung tissue and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cathepsin C/DPPI at approximately 20-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Cathepsin C/DPPI in Mouse Thymus. Cathepsin C/DPPI was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Cathepsin C/
DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). Specific staining was localized to thymocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse Cathepsin C/DPPI by Simple WesternTM. Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for Cathepsin C/DPPI at approximately 35 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin C/DPPI

Cathepsin C is a cysteine protease of the papain family (1). Cathepsin C sequentially removes dipeptides from the free N-termini of proteins and peptides. It has broad specificity except that it does not cleave a basic amino acid (Arg or Lys) in the N-terminal position or Pro on either side of the scissle bond. It requires halide ions for activity. The pro form contains a pro peptide and a catalytic region, which can be further processed into heavy/ alpha and light/ beta chains that are linked by a disulfide bond. It is broadly distributed. Cathepsin C plays a role in the lysosomal degradation. It also functions as a key enzyme in the activation of granule serine proteases in cytotoxic T lymphocytes and natural killer cells (granzymes A and B), mast cells (tryptase and chymase), and neutrophils (Cathepsin G and elastase) by removing their N-terminal activation dipeptides (2).

  • References:
    1. Turk, B. et al. (2004) in Handbook of Proteolytic Enzymes. Barrett, et al. eds. p. 1192, Academic Press, San Diego.
    2. Dahl, S.W. et al. (2001) Biochemistry 40:1671.
  • Entrez Gene IDs:
    1075 (Human); 13032 (Mouse); 25423 (Rat)
  • Alternate Names:
    Cathepsin C; cathepsin CEC 3.4.14.1; Cathepsin J; CPPIHMS; CTSC; dipeptidyl peptidase 1; Dipeptidyl peptidase I; Dipeptidyl transferase; dipeptidyl-peptidase I; DPP1; DPPI; DPP-I; JP; JPD; PALS; PLS
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Lysosomal protein turnover contributes to the acquisition of TGFbeta-1 induced invasive properties of mammary cancer cells.
    Authors: Kern U, Wischnewski V, Biniossek M, Schilling O, Reinheckel T
    Mol Cancer, 2015;14(0):39.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer.
    Authors: Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K
    PLoS ONE, 2015;10(4):e0120348.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-OCT embedded
  3. The endolysosomal cysteine cathepsins L and K are involved in macrophage-mediated clearance of Staphylococcus aureus and the concomitant cytokine induction.
    Authors: Muller S, Faulhaber A, Sieber C, Pfeifer D, Hochberg T, Gansz M, Deshmukh S, Dauth S, Brix K, Saftig P, Peters C, Henneke P, Reinheckel T
    FASEB J, 2014;28(1):162-75.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Up-regulation of microglial cathepsin C expression and activity in lipopolysaccharide-induced neuroinflammation.
    Authors: Fan K, Wu X, Fan B, Li N, Lin Y, Yao Y, Ma J
    J Neuroinflammation, 2012;9(1):96.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  5. A role for serglycin proteoglycan in mast cell apoptosis induced by a secretory granule-mediated pathway.
    Authors: Melo FR, Waern I, Ronnberg E, Abrink M, Lee DM, Schlenner SM, Feyerabend TB, Rodewald HR, Turk B, Wernersson S, Pejler G
    J. Biol. Chem., 2011;286(7):5423-33.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. Mice deficient in LMAN1 exhibit FV and FVIII deficiencies and liver accumulation of {alpha}1-antitrypsin.
    Authors: Zhang B, Zheng C, Zhu M, Tao J, Vasievich MP, Baines A, Kim J, Schekman R, Kaufman RJ, Ginsburg D
    Blood, 2011;118(12):3384-91.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  7. Gene targeting of the cysteine peptidase cathepsin H impairs lung surfactant in mice.
    Authors: Buhling F, Kouadio M, Chwieralski CE, Kern U, Hohlfeld JM, Klemm N, Friedrichs N, Roth W, Deussing JM, Peters C, Reinheckel T
    PLoS ONE, 2011;6(10):e26247.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  8. Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer.
    Authors: Shree T, Olson OC, Elie BT
    Genes Dev., 2011;25(23):2465-79.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
Cell and Tissue Staining Kits
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Immunohistochemistry Reagents
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

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Goat IgG HRP-conjugated Antibody

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Goat IgG (H+L) APC-conjugated Antibody

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Goat IgG Antibody

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Goat IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

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Chicken Anti-Goat IgG Biotinylated Antibody

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Average Rating: 3 (Based on 1 review)

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Summary

ApplicationELISA
Sample TestedPeritoneal resident macrophages
SpeciesMouse

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