Mouse/Rat FABP2/I‑FABP Antibody
R&D Systems | Catalog # AF1486
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant rat FABP2/I‑FABP
Met1-Glu132
Accession # P02693
Met1-Glu132
Accession # P02693
Specificity
Detects mouse and rat FABP2/I‑FABP in Western blots. Detects rat FABP2/I‑FABP in direct ELISAs. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant rat FABP1, recombinant human FABP3, recombinant mouse (rm) FABP4, and rmFABP5 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat FABP2/I‑FABP Antibody
Detection of Mouse FABP2/I‑FABP by Western Blot.
Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat FABP2/I-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1486) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP2/I-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FABP2/I‑FABP in Rat Mesenchymal Stem Cells.
FABP2/I-FABP was detected in immersion fixed rat mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse/Rat FABP2/I-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1486) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
FABP2/I‑FABP in Mouse Intestine.
FABP2/I-FABP was detected in immersion fixed frozen sections of adult mouse intestine using Goat Anti-Mouse/Rat FABP2/I-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1486) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to intestinal epithelia. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Mouse FABP2/I‑FABP by Simple WesternTM.
Simple Western lane view shows lysates of mouse small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP2/I-FABP at approximately 18 kDa (as indicated) using 20 µg/mL of Goat Anti-Mouse/Rat FABP2/I-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1486) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Mouse/Rat FABP2/I‑FABP Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed rat mesenchymal stem cells differentiated to adipocytes and Caco‑2 human colorectal adenocarcinoma cell line
Sample: Immersion fixed rat mesenchymal stem cells differentiated to adipocytes and Caco‑2 human colorectal adenocarcinoma cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of adult mouse intestine
Sample: Immersion fixed frozen sections of adult mouse intestine
Simple Western
20 µg/mL
Sample: Mouse Small Intestine Issue
Sample: Mouse Small Intestine Issue
Western Blot
1 µg/mL
Sample: Mouse small intestine tissue
Sample: Mouse small intestine tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: FABP2/I-FABP
Long Name
Fatty Acid-Binding Protein 2/Intestinal FABP
Alternate Names
I-FABP, IFABP, Intestinal FABP
Gene Symbol
FABP2
UniProt
Additional FABP2/I-FABP Products
Product Documents for Mouse/Rat FABP2/I‑FABP Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse/Rat FABP2/I‑FABP Antibody
For research use only
Related Research Areas
Citations for Mouse/Rat FABP2/I‑FABP Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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