Mouse/Rat FABP2/I-FABP Antibody Summary
Met1-Glu132
Accession # P02693
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse FABP2/I‑FABP by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat FABP2/I-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1486) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP2/I-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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FABP2/I‑FABP in Rat Mesenchymal Stem Cells. FABP2/I-FABP was detected in immersion fixed rat mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse/Rat FABP2/I-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1486) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
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FABP2/I‑FABP in Mouse Intestine. FABP2/I-FABP was detected in immersion fixed frozen sections of adult mouse intestine using Goat Anti-Mouse/Rat FABP2/I-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1486) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to intestinal epithelia. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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Detection of Mouse FABP2/I‑FABP by Simple WesternTM. Simple Western lane view shows lysates of mouse small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP2/I-FABP at approximately 18 kDa (as indicated) using 20 µg/mL of Goat Anti-Mouse/Rat FABP2/I-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1486) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP2/I-FABP
FABP2, also named I-FABP and gFABP, is a member of the intracellular fatty acid binding protein family. It is highly expressed in the intestine. FABP2 binds fatty acid in a non-covalent 1:1 complex to chaperone the lipids to cellular enzymes for metabolism and signal transduction.
Product Datasheets
Citation for Mouse/Rat FABP2/I-FABP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Nkx2.2 is expressed in a subset of enteroendocrine cells with expanded lineage potential.
Authors: Gross S, Balderes D, Liu J et al.
Am J Physiol Gastrointest Liver Physiol
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