Mouse/Rat FABP2/I-FABP Antibody

Catalog # Availability Size / Price Qty
AF1486
AF1486-SP

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Detection of Mouse FABP2/I‑FABP by Western Blot.
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Mouse/Rat FABP2/I-FABP Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat FABP2/I‑FABP in Western blots. Detects rat FABP2/I‑FABP in direct ELISAs. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant rat FABP1, recombinant human FABP3, recombinant mouse (rm) FABP4, and rmFABP5 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat FABP2/I‑FABP
Met1-Glu132
Accession # P02693
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
20 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse FABP2/I-FABP antibody by Western Blot. View Larger

Detection of Mouse FABP2/I‑FABP by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat FABP2/I-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1486) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP2/I-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry FABP2/I-FABP antibody in Rat Mesenchymal Stem Cells by Immunocytochemistry (ICC). View Larger

FABP2/I‑FABP in Rat Mesenchymal Stem Cells. FABP2/I-FABP was detected in immersion fixed rat mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse/Rat FABP2/I-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1486) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunohistochemistry FABP2/I-FABP antibody in Mouse Intestine by Immunohistochemistry (IHC-Fr). View Larger

FABP2/I‑FABP in Mouse Intestine. FABP2/I-FABP was detected in immersion fixed frozen sections of adult mouse intestine using Goat Anti-Mouse/Rat FABP2/I-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1486) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to intestinal epithelia. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Mouse FABP2/I-FABP antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse FABP2/I‑FABP by Simple WesternTM. Simple Western lane view shows lysates of mouse small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP2/I-FABP at approximately 18 kDa (as indicated) using 20 µg/mL of Goat Anti-Mouse/Rat FABP2/I-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1486) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP2/I-FABP

FABP2, also named I-FABP and gFABP, is a member of the intracellular fatty acid binding protein family. It is highly expressed in the intestine. FABP2 binds fatty acid in a non-covalent 1:1 complex to chaperone the lipids to cellular enzymes for metabolism and signal transduction.

Long Name
Fatty Acid-Binding Protein 2/Intestinal FABP
Entrez Gene IDs
2169 (Human); 25598 (Rat)
Alternate Names
FABP2; FABPI; fatty acid binding protein 2, intestinal; Fatty acid-binding protein 2; fatty acid-binding protein, intestinal; IFABP; I-FABP; I-FABPMGC133132; Intestinal FABP; Intestinal-type fatty acid-binding protein

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