PDX-1/IPF1 Antibody

R&D Systems | Catalog # AF2517

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PDX-1/IPF1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse PDX‑1/IPF1
Met1-Arg284
Accession # P52946

Specificity

Detects mouse PDX‑1/IPF1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PDX-1/IPF1 Antibody

Detection of Mouse/Rat PDX‑1/IPF1 antibody by Western Blot.

Detection of Mouse/Rat PDX‑1/IPF1 by Western Blot.

Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2517) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDX-1/IPF1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
PDX-1/IPF1 antibody in beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC).

PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line.

PDX-1/IPF1 was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Goat Anti-Mouse/Rat PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2517) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Mouse PDX‑1/IPF1 antibody by Simple WesternTM.

Detection of Mouse PDX‑1/IPF1 by Simple WesternTM.

Simple Western lane view shows lysates of beta TC-6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PDX-1/IPF1 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2517) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
PDX-1/IPF1 antibody in Mouse Pancreas by Immunohistochemistry (IHC-Fr).

PDX‑1/IPF1 in Mouse Pancreas.

PDX-1/IPF1 was detected in perfusion fixed frozen sections of mouse pancreas using Goat Anti-Mouse/Rat PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2517) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for PDX-1/IPF1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed beta TC-6 mouse beta cell insulinoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse pancreas

Simple Western

10 µg/mL
Sample: beta TC‑6 mouse beta cell insulinoma cell line

Western Blot

1 µg/mL
Sample: beta TC‑6 mouse beta cell insulinoma cell line

Reviewed Applications

Read 1 review rated 3 using AF2517 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: PDX-1/IPF1

PDX-1 (Pancreas-duodenum homeobox 1; also IPF-1, IDX-1, STF-1, GSF and IUF-1) is a 42 kDa member of the IPF-1 subfamily, ANTP homeobox family of proteins. It is expressed in developing pancreas, plus pancreatic beta -cells, and duodenal crypt epithelium. In concert with HNF6, PDX-1 drives pancreatic endocrine cell formation by regulating the expression of neurogenin-3. In adult beta -cells, PDX-1 binds to PCIF-1 to regulate insulin production. Mouse PDX-1 is 284 amino acids (aa) in length (Accession # P52946). It contains one transactivation domain (aa 13‑73) a PBX1 P-P-M-W-K dimerization motif (aa 119‑124), and a DNA-binding homeodomain with an embedded NLS (aa 148‑206). Full-length mouse PDX-1 shares 94% and 88% aa identity with rat and human PDX-1, respectively.

Long Name

Pancreas/Duodenum Homeobox-1

Alternate Names

IDX1, IPF1

Entrez Gene IDs

3651 (Human); 18609 (Mouse)

Gene Symbol

PDX1

UniProt

P52946

Additional PDX-1/IPF1 Products

Product Documents for PDX-1/IPF1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for PDX-1/IPF1 Antibody

For research use only

Citations for PDX-1/IPF1 Antibody

Customer Reviews for PDX-1/IPF1 Antibody (1)

3 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
0%
3 Stars
100%
2 Stars
0%
1 Stars
0%

Have you used PDX-1/IPF1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Mouse/Rat PDX-1/IPF1 Antibody
    Name: Jonatan Dereke
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Pancreas tissue
    Species: Rat
    Verified Customer | Posted 04/29/2020
    Paraffin embeded rat pancreatic tissue stained for insulin (red) and PDX-1 (AF2517, green).
    We used this antibody at a 1:400 dilution for our assays.
    PDX-1/IPF1 Antibody AF2517

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies