Plexin A3 (also known as SEX) is a 220 kDa member of the Plexin A subfamily of semaphorin receptors. Mature mouse Plexin A3 is predicted to be an 1853 amino acid (aa) type I transmembrane glycoprotein that contains a 1202 aa extracellular domain (ECD) and a 629 aa cytoplasmic region. The ECD contains one semaphorin domain (aa 34-473), three plexin-semaphorin-integrin (PSI) domains (aa 492-830), and four lg-like plexin-transcription factor (IPT) repeats (aa 841-1209). Plexin A3 interacts with neuropilins 1 and 2, and binds class 3 semaphorins. Over aa 35-150, mouse Plexin A3 is 99% and 97% aa identical to rat and human Plexin A3, respectively.
Mouse/Rat Plexin A3 Antibody
R&D Systems | Catalog # AF4075
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant mouse Plexin A3
Leu34-Asp150
Accession # P70208
Leu34-Asp150
Accession # P70208
Specificity
Detects mouse Plexin A3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Plexin A1, rmPlexin A2, recombinant human (rh) Plexin B1, rhPlexin C1, and rhPlexin D1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat Plexin A3 Antibody
Plexin A3 in Mouse Spinal Cord.
Plexin A3 was detected in immersion fixed frozen sections of 13 d.p.c. embryonic mouse spinal cord using Goat Anti-Mouse Plexin A3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4075) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Plexin A3 in Rat Brain (Cortex).
Plexin A3 was detected in immersion fixed paraffin-embedded sections of rat brain (cortex) using Goat Anti-Mouse/Rat Plexin A3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4075) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuroglia. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Mouse/Rat Plexin A3 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of 13 d.p.c. embryonic mouse spinal cord and immersion fixed paraffin-embedded sections of rat brain (cortex)
Sample: Immersion fixed frozen sections of 13 d.p.c. embryonic mouse spinal cord and immersion fixed paraffin-embedded sections of rat brain (cortex)
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Plexin A3
Sample: Recombinant Mouse Plexin A3
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Plexin A3
Alternate Names
Plxn3, PLXN4, PLXNA3, SEX, XAP-6
Gene Symbol
PLXNA3
UniProt
Additional Plexin A3 Products
Product Documents for Mouse/Rat Plexin A3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse/Rat Plexin A3 Antibody
For research use only
Citations for Mouse/Rat Plexin A3 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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