Detects mouse Plexin A3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Plexin A1, rmPlexin A2, recombinant human (rh) Plexin B1, rhPlexin C1, and rhPlexin D1 is observed.
Polyclonal Goat IgG
E. coli-derived recombinant mouse Plexin A3 Leu34-Asp150 Accession # P70208
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Plexin A3 in Mouse Spinal Cord.
Plexin A3 was detected in immersion fixed frozen sections of mouse spinal cord using Goat Anti-Mouse Plexin A3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4075) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Plexin A3 in Rat Spinal Cord.
Plexin A3 was detected in immersion fixed frozen sections of embryonic rat spinal cord using Goat Anti-Mouse Plexin A3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4075) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plexin A3
Plexin A3 (also known as SEX) is a 220 kDa member of the Plexin A subfamily of semaphorin receptors. Mature mouse Plexin A3 is predicted to be an 1853 amino acid (aa) type I transmembrane glycoprotein that contains a 1202 aa extracellular domain (ECD) and a 629 aa cytoplasmic region. The ECD contains one semaphorin domain (aa 34-473), three plexin-semaphorin-integrin (PSI) domains (aa 492-830), and four lg-like plexin-transcription factor (IPT) repeats (aa 841-1209). Plexin A3 interacts with neuropilins 1 and 2, and binds class 3 semaphorins. Over aa 35-150, mouse Plexin A3 is 99% and 97% aa identical to rat and human Plexin A3, respectively.
Entrez Gene IDs:
55558 (Human); 18846 (Mouse); 309280 (Rat)
HSSEXGENE; Plexin A3; plexin-4; plexin-A3; Plxn3; PLXN4; PLXNA3; Semaphorin receptor SEX; Sex chromosome X transmembrane protein of HGF receptor family 3; SEX; SEXPLEXIN-A3,6.3; XAP-6
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