Mouse S100A9 Antibody

Catalog # Availability Size / Price Qty
AF2065
AF2065-SP
Mouse S100A9 Antibody in Data
5 Images
Product Details
Citations (19)
FAQs
Supplemental Products
Reviews (1)

Mouse S100A9 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse S100A9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross‑reactivity with recombinant mouse S100A10 and recombinant human S100B is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse S100A9
Ala2-Lys113
Accession # P31725
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
2 µg/mL
See below
Immunohistochemistry
0.3-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse S100A9 antibody by Western Blot. View Larger

Detection of Mouse S100A9 by Western Blot. Western blot shows lysate of mouse lung tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry S100A9 antibody in XB2 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

S100A9 in XB2 Mouse Cell Line. S100A9 was detected in immersion fixed XB2 mouse teratoma keratinocyte cell line using 10 µg/mL Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counte-rstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry S100A9 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

S100A9 in Mouse Splenocytes. S100A9 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry S100A9 antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr). View Larger

S100A9 in Mouse Spleen. S100A9 was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A9

S100A9, also known as calgranulin B, migration inhibitory factor-related protein 14 (MRP14), belongs to the S100 family of calcium binding proteins of the EF-hand type. It is highly expressed in myeloid cells and other cell types including keratinocytes, epithelial cells and endothelial cells. S100A9 is a cytosolic protein that can also be released into the extracellular milieu. It is associated with various inflammatory diseases.

Long Name
S100 Calcium Binding Protein A9
Entrez Gene IDs
6280 (Human); 20202 (Mouse)
Alternate Names
CAGBMigration inhibitory factor-related protein 14; Calgranulin B; calgranulin-B; Calprotectin L1H subunit; CFAGMRP-14,60B8AG; CGLB; L1AG; LIAG; MAC387; MIF; MRP-14; MRP14Leukocyte L1 complex heavy chain; NIF; P14; protein S100-A9; S100 calcium binding protein A9 (calgranulin B); S100 calcium binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100 calcium-binding protein A9; S100A9

Product Datasheets

Citations for Mouse S100A9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. C-terminal truncation of IFN-? inhibits proinflammatory macrophage responses and is deficient in autoimmune disease
    Authors: A Dufour, CL Bellac, U Eckhard, N Solis, T Klein, R Kappelhoff, N Fortelny, P Jobin, J Rozmus, J Mark, P Pavlidis, V Dive, SJ Barbour, CM Overall
    Nat Commun, 2018;9(1):2416.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Induction of S100A9 homodimer formation in�vivo
    Authors: E Källberg, S Tahvili, F Ivars, T Leanderson
    Biochem. Biophys. Res. Commun., 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Beneficial Effects of Sodium Phenylbutyrate Administration During Infection with Salmonella enterica serovar Typhimurium
    Infect Immun, 2016;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Oncostatin M overexpression induces skin inflammation but is not required in the mouse model of imiquimod-induced psoriasis-like inflammation
    Eur J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Influenza Virus Affects Intestinal Microbiota and Secondary Salmonella Infection in the Gut through Type I Interferons
    PLoS Pathog, 2016;12(5):e1005572.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Loss of JUNB/AP-1 promotes invasive prostate cancer.
    Authors: Thomsen M, Bakiri L, Hasenfuss S, Wu H, Morente M, Wagner E
    Cell Death Differ, 2015;22(4):574-82.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Novel whole blood assay for phenotyping platelet reactivity in mice identifies ICAM-1 as a mediator of platelet-monocyte interaction.
    Authors: Armstrong P, Kirkby N, Chan M, Finsterbusch M, Hogg N, Nourshargh S, Warner T
    Blood, 2015;126(10):e11-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Confocal Microscopy
  8. S100A9 knockout decreases the memory impairment and neuropathology in crossbreed mice of Tg2576 and S100A9 knockout mice model.
    Authors: Kim H, Chang K, Ha T, Kim J, Ha S, Shin K, Moon C, Nacken W, Kim H, Suh Y
    PLoS ONE, 2014;9(2):e88924.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Vaginal epithelial cell-derived S100 alarmins induced by Candida albicans via pattern recognition receptor interactions are sufficient but not necessary for the acute neutrophil response during experimental vaginal candidiasis.
    Authors: Yano J, Palmer G, Eberle K, Peters B, Vogl T, McKenzie A, Fidel P
    , 2014;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  10. The cytokine IL-22 promotes pathogen colonization by suppressing related commensal bacteria.
    Authors: Behnsen J, Jellbauer S, Wong C, Edwards R, George M, Ouyang W, Raffatellu M
    Immunity, 2014;40(2):262-73.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. Central role of conventional dendritic cells in regulation of bone marrow release and survival of neutrophils.
    Authors: Jiao, Jingjing, Dragomir, Ana-Cris, Kocabayoglu, Peri, Rahman, Adeeb H, Chow, Andrew, Hashimoto, Daigo, Leboeuf, Marylene, Kraus, Thomas, Moran, Thomas, Carrasco-Avino, Gonzalo, Friedman, Scott L, Merad, Miriam, Aloman, Costica
    J Immunol, 2014;192(7):3374-82.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Tumor necrosis factor-alpha blocks differentiation and enhances suppressive activity of immature myeloid cells during chronic inflammation.
    Authors: Sade-Feldman M, Kanterman J, Ish-Shalom E, Elnekave M, Horwitz E, Baniyash M
    Immunity, 2013;38(3):541-54.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Dietary zinc deficiency fuels esophageal cancer development by inducing a distinct inflammatory signature.
    Authors: Taccioli, C, Chen, H, Jiang, Y, Liu, X P, Huang, K, Smalley, K J, Farber, J L, Croce, C M, Fong, L Y
    Oncogene, 2012;31(42):4550-8.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. Proximal fluid proteome profiling of mouse colon tumors reveals biomarkers for early diagnosis of human colorectal cancer.
    Authors: Fijneman RJ, de Wit M, Pourghiasian M, Piersma SR, Pham TV, Warmoes MO, Lavaei M, Piso C, Smit F, Delis-van Diemen PM, van Turenhout ST, Terhaar sive Droste JS, Mulder CJ, Blankenstein MA, Robanus-Maandag EC, Smits R, Fodde R, van Hinsbergh VW, Meijer GA, Jimenez CR
    Clin. Cancer Res., 2012;18(9):2613-24.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. MMP-8 Deficiency Increases TLR/RAGE Ligands S100A8 and S100A9 and Exacerbates Lung Inflammation during Endotoxemia.
    Authors: Gonzalez-Lopez A, Aguirre A, Lopez-Alonso I, Amado L, Astudillo A, Fernandez-Garcia MS, Suarez MF, Batalla-Solis E, Colado E, Albaiceta GM
    PLoS ONE, 2012;7(6):e39940.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  16. The Acute Neutrophil Response Mediated by S100 Alarmins during Vaginal Candida Infections Is Independent of the Th17-Pathway.
    Authors: Yano, Junko, Kolls, Jay K, Happel, Kyle I, Wormley, Floyd, Wozniak, Karen L, Fidel, Paul L J
    PLoS ONE, 2012;7(9):e46311.
    Species: Mouse
    Sample Types: Vaginal Lavage Fluid
    Applications: ELISA Development
  17. Myeloid-Derived Suppressor Cells Infiltrate the Heart in Acute Trypanosoma cruzi Infection.
    Authors: Cuervo H, Guerrero NA, Carbajosa S, Beschin A, De Baetselier P, Girones N, Fresno M
    J. Immunol., 2011;187(5):2656-65.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  18. Mechanisms of Urokinase Plasminogen Activator (uPA)-mediated Atherosclerosis: ROLE OF THE uPA RECEPTOR AND S100A8/A9 PROTEINS.
    Authors: Farris SD, Hu JH, Krishnan R, Emery I, Chu T, DU L, Kremen M, Dichek HL, Gold E, Ramsey SA, Dichek DA
    J. Biol. Chem., 2011;286(25):22665-77.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  19. Initial delay in the immune response to Francisella tularensis is followed by hypercytokinemia characteristic of severe sepsis and correlating with upregulation and release of damage-associated molecular patterns.
    Authors: Mares CA, Ojeda SS, Morris EG, Li Q, Teale JM
    Infect. Immun., 2008;76(7):3001-10.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Functional Assay

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse S100A9 Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Mouse S100A9 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse S100A9 Antibody
By Anonymous on 05/03/2017
Application: WB Sample Tested: mouse feces Species: Mouse

S100A9 detection in mouse feces at 1:500, 1:1000 and 1:2000 dilution.
Incubation time was 2 hrs at RT. Antibody in 1% milk TBST