Mouse S100A9 Antibody

(17 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse S100A9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross‑reactivity with recombinant mouse S100A10 and recombinant human S100B is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse S100A9
    Ala2-Lys113
    Accession # P31725
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Simple Western
    2 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse S100A9 by Western Blot. Western blot shows lysate of mouse lung tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
S100A9 in XB2 Mouse Cell Line. S100A9 was detected in immersion fixed XB2 mouse teratoma keratinocyte cell line using 10 µg/mL Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counte­rstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Mouse S100A9 by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue and mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A9 at approximately 20 kDa (as indicated) using 2 µg/mL of Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A9
S100A9, also known as calgranulin B, migration inhibitory factor-related protein 14 (MRP14), belongs to the S100 family of calcium binding proteins of the EF-hand type. It is highly expressed in myeloid cells and other cell types including keratinocytes, epithelial cells and endothelial cells. S100A9 is a cytosolic protein that can also be released into the extracellular milieu. It is associated with various inflammatory diseases.
  • Long Name:
    S100 Calcium Binding Protein A9
  • Entrez Gene IDs:
    6280 (Human); 20202 (Mouse)
  • Alternate Names:
    CAGBMigration inhibitory factor-related protein 14; Calgranulin B; calgranulin-B; Calprotectin L1H subunit; CFAGMRP-14,60B8AG; CGLB; L1AG; LIAG; MAC387; MIF; MRP-14; MRP14Leukocyte L1 complex heavy chain; NIF; P14; protein S100-A9; S100 calcium binding protein A9 (calgranulin B); S100 calcium binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100 calcium-binding protein A9; S100A9
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Oncostatin M overexpression induces skin inflammation but is not required in the mouse model of imiquimod-induced psoriasis-like inflammation
    Eur J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  2. Influenza Virus Affects Intestinal Microbiota and Secondary Salmonella Infection in the Gut through Type I Interferons
    PLoS Pathog, 2016;12(5):e1005572.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Beneficial Effects of Sodium Phenylbutyrate Administration During Infection with Salmonella enterica serovar Typhimurium
    Infect Immun, 2016;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. Novel whole blood assay for phenotyping platelet reactivity in mice identifies ICAM-1 as a mediator of platelet-monocyte interaction.
    Authors: Armstrong P, Kirkby N, Chan M, Finsterbusch M, Hogg N, Nourshargh S, Warner T
    Blood, 2015;126(10):e11-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Confocal Microscopy
  5. Loss of JUNB/AP-1 promotes invasive prostate cancer.
    Authors: Thomsen M, Bakiri L, Hasenfuss S, Wu H, Morente M, Wagner E
    Cell Death Differ, 2015;22(4):574-82.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  6. S100A9 knockout decreases the memory impairment and neuropathology in crossbreed mice of Tg2576 and S100A9 knockout mice model.
    Authors: Kim H, Chang K, Ha T, Kim J, Ha S, Shin K, Moon C, Nacken W, Kim H, Suh Y
    PLoS ONE, 2014;9(2):e88924.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  7. Vaginal epithelial cell-derived S100 alarmins induced by Candida albicans via pattern recognition receptor interactions are sufficient but not necessary for the acute neutrophil response during experimental vaginal candidiasis.
    Authors: Yano J, Palmer G, Eberle K, Peters B, Vogl T, McKenzie A, Fidel P
    , 2014;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  8. The cytokine IL-22 promotes pathogen colonization by suppressing related commensal bacteria.
    Authors: Behnsen J, Jellbauer S, Wong C, Edwards R, George M, Ouyang W, Raffatellu M
    Immunity, 2014;40(2):262-73.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  9. Central role of conventional dendritic cells in regulation of bone marrow release and survival of neutrophils.
    Authors: Jiao, Jingjing, Dragomir, Ana-Cris, Kocabayoglu, Peri, Rahman, Adeeb H, Chow, Andrew, Hashimoto, Daigo, Leboeuf, Marylene, Kraus, Thomas, Moran, Thomas, Carrasco-Avino, Gonzalo, Friedman, Scott L, Merad, Miriam, Aloman, Costica
    J Immunol, 2014;192(7):3374-82.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IF
  10. Tumor necrosis factor-alpha blocks differentiation and enhances suppressive activity of immature myeloid cells during chronic inflammation.
    Authors: Sade-Feldman M, Kanterman J, Ish-Shalom E, Elnekave M, Horwitz E, Baniyash M
    Immunity, 2013;38(3):541-54.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  11. MMP-8 Deficiency Increases TLR/RAGE Ligands S100A8 and S100A9 and Exacerbates Lung Inflammation during Endotoxemia.
    Authors: Gonzalez-Lopez A, Aguirre A, Lopez-Alonso I, Amado L, Astudillo A, Fernandez-Garcia MS, Suarez MF, Batalla-Solis E, Colado E, Albaiceta GM
    PLoS ONE, 2012;7(6):e39940.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  12. The Acute Neutrophil Response Mediated by S100 Alarmins during Vaginal Candida Infections Is Independent of the Th17-Pathway.
    Authors: Yano, Junko, Kolls, Jay K, Happel, Kyle I, Wormley, Floyd, Wozniak, Karen L, Fidel, Paul L J
    PLoS ONE, 2012;7(9):e46311.
    Species: Mouse
    Sample Type: Vaginal Lavage
    Application: ELISA Development
  13. Dietary zinc deficiency fuels esophageal cancer development by inducing a distinct inflammatory signature.
    Authors: Taccioli, C, Chen, H, Jiang, Y, Liu, X P, Huang, K, Smalley, K J, Farber, J L, Croce, C M, Fong, L Y
    Oncogene, 2012;31(42):4550-8.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
  14. Proximal fluid proteome profiling of mouse colon tumors reveals biomarkers for early diagnosis of human colorectal cancer.
    Authors: Fijneman RJ, de Wit M, Pourghiasian M, Piersma SR, Pham TV, Warmoes MO, Lavaei M, Piso C, Smit F, Delis-van Diemen PM, van Turenhout ST, Terhaar sive Droste JS, Mulder CJ, Blankenstein MA, Robanus-Maandag EC, Smits R, Fodde R, van Hinsbergh VW, Meijer GA, Jimenez CR
    Clin. Cancer Res., 2012;18(9):2613-24.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  15. Myeloid-Derived Suppressor Cells Infiltrate the Heart in Acute Trypanosoma cruzi Infection.
    Authors: Cuervo H, Guerrero NA, Carbajosa S, Beschin A, De Baetselier P, Girones N, Fresno M
    J. Immunol., 2011;187(5):2656-65.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  16. Mechanisms of Urokinase Plasminogen Activator (uPA)-mediated Atherosclerosis: ROLE OF THE uPA RECEPTOR AND S100A8/A9 PROTEINS.
    Authors: Farris SD, Hu JH, Krishnan R, Emery I, Chu T, DU L, Kremen M, Dichek HL, Gold E, Ramsey SA, Dichek DA
    J. Biol. Chem., 2011;286(25):22665-77.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  17. Initial delay in the immune response to Francisella tularensis is followed by hypercytokinemia characteristic of severe sepsis and correlating with upregulation and release of damage-associated molecular patterns.
    Authors: Mares CA, Ojeda SS, Morris EG, Li Q, Teale JM
    Infect. Immun., 2008;76(7):3001-10.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Functional Assay
Expand to show all 17 Citations
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Mouse S100A9 Antibody AF2065
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Excellent
 WB Mouse Anonymous 05/03/2017
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Western Blot Mouse S100A9 Antibody AF2065
Western Blot: Mouse S100A9 Antibody [AF2065]

Summary

ApplicationWestern Blot
Sample Testedmouse feces
SpeciesMouse

Other Experimental Details

Other Experimental DetailsS100A9 detection in mouse feces at 1:500, 1:1000 and 1:2000 dilution.Incubation time was 2 hrs at RT. Antibody in 1% milk TBST

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