Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Confocal Microscopy, ELISA Development, Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all S100A9 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse S100A9
Ala2-Lys113
Accession # P31725

Specificity

Detects mouse S100A9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross‑reactivity with recombinant mouse S100A10 and recombinant human S100B is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse S100A9 Antibody

Detection of Mouse S100A9 antibody by Western Blot.

Detection of Mouse S100A9 by Western Blot.

Western blot shows lysate of mouse lung tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

S100A9 antibody in XB2 Mouse Cell Line by Immunocytochemistry (ICC).

S100A9 in XB2 Mouse Cell Line.

S100A9 was detected in immersion fixed XB2 mouse teratoma keratinocyte cell line using 10 µg/mL Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counte-rstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

S100A9 antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

S100A9 in Mouse Splenocytes.

S100A9 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

S100A9 antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr).

S100A9 in Mouse Spleen.

S100A9 was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Mouse S100A9 antibody by Simple Western™

Detection of Mouse S100A9 by Simple Western™

Simple Western lane view shows lysates of mouse lung tissue and mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A9 at approximately 20 kDa (as indicated) using 2 µg/mL of Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of S100A9 by Western Blot

Detection of S100A9 by Western Blot

S100A8/A9 proteins augment differentiation and osteoclastic activity derived from iOCPs but not from hOCPs. a Expression of S100A8, S100A9 and RAGE in sorted control and inflamed BM iOCPs and hOCPs. The protein phosphatase 2A catalytic subunit (PP2Ac) is shown as a loading control. b Densitometry measurements of three biological repeats (no statistical analysis presented). c Sorted control and inflamed BM iOCPs and hOCPs (5 × 104) were cultured on the Osteo assay surface with or without anti-RAGE blocking antibodies (bar: 50 µm). d Pit area quantitation. e Sorted iOCPs and hOCPs (5 × 104) from the BM of control mice were cultured on the Osteo assay surface in combination with a recombinant S100A8/A9 heterodimer and anti-RAGE blocking antibodies as indicated (bar: 50 µm). f Pit area quantitation. c–f Depict representative results for two independent experiments, n = 5 for each group. Line: median, box: 25th-75th percentile, whiskers: range. *P < 0.05 (Mann–Whitney test and Holm multiplicity correction) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35396510), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse S100A9 by Immunohistochemistry

Detection of Mouse S100A9 by Immunohistochemistry

iASPP deficient keratinocytes induce pro-inflammatory gene expression in vitro and attract macrophages in vivo(A) qPCR analysis of mRNA expression levels of inflammatory genes (and iASPP gene Ppp1r13l) in iASPP WT and KO primary keratinocytes at 0, 1, and 6 h after treatment with TNF-alpha. Values are mean + SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; n = 3 biological replicates.(B) Left, IHC staining of S100a8 and S100a9 in untreated skin sections from iASPP WT and KO mice (adjacent sections). Scale bar, 50 μm. Right, IB of iASPP and S100a9 expression levels in iASPP WT (−tamoxifen) and KO (+tamoxifen) primary keratinocytes.(C) H&E analysis of acetone- or TPA-treated skin sections from iASPP WT and KO mice. Scale bar, 50 μm. Histograms below show epidermal thickness in the same samples. Values are mean ± SD. n = 3 (WT) and n = 4 (KO) mice in acetone cohort; n = 4 (WT), n = 3 (KO) mice in TPA cohort.(D) IHC staining of F4/80-positive macrophages in acetone- or TPA-treated skin sections from iASPP WT and KO mice. Scale bar, 50 μm. Histograms below show quantification of the same samples. Values are mean + SD. Same cohort as in (C).See STAR Methods for p calculations for (A), (C), and (D). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36261000), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse S100A9 by Immunohistochemistry

Detection of Mouse S100A9 by Immunohistochemistry

iASPP deficient keratinocytes induce pro-inflammatory gene expression in vitro and attract macrophages in vivo(A) qPCR analysis of mRNA expression levels of inflammatory genes (and iASPP gene Ppp1r13l) in iASPP WT and KO primary keratinocytes at 0, 1, and 6 h after treatment with TNF-alpha. Values are mean + SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; n = 3 biological replicates.(B) Left, IHC staining of S100a8 and S100a9 in untreated skin sections from iASPP WT and KO mice (adjacent sections). Scale bar, 50 μm. Right, IB of iASPP and S100a9 expression levels in iASPP WT (−tamoxifen) and KO (+tamoxifen) primary keratinocytes.(C) H&E analysis of acetone- or TPA-treated skin sections from iASPP WT and KO mice. Scale bar, 50 μm. Histograms below show epidermal thickness in the same samples. Values are mean ± SD. n = 3 (WT) and n = 4 (KO) mice in acetone cohort; n = 4 (WT), n = 3 (KO) mice in TPA cohort.(D) IHC staining of F4/80-positive macrophages in acetone- or TPA-treated skin sections from iASPP WT and KO mice. Scale bar, 50 μm. Histograms below show quantification of the same samples. Values are mean + SD. Same cohort as in (C).See STAR Methods for p calculations for (A), (C), and (D). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36261000), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse S100A9 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed XB2 mouse teratoma keratinocyte cell line and mouse splenocytes

Immunohistochemistry

0.3-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse spleen

Simple Western

2 µg/mL
Sample: Mouse lung tissue and mouse spleen tissue

Western Blot

0.2 µg/mL
Sample: Mouse lung tissue

Reviewed Applications

Read 5 reviews rated 4.6 using AF2065 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: S100A9

S100A9, also known as calgranulin B, migration inhibitory factor-related protein 14 (MRP14), belongs to the S100 family of calcium binding proteins of the EF-hand type. It is highly expressed in myeloid cells and other cell types including keratinocytes, epithelial cells and endothelial cells. S100A9 is a cytosolic protein that can also be released into the extracellular milieu. It is associated with various inflammatory diseases.

Long Name

S100 Calcium Binding Protein A9

Alternate Names

Calgranulin B, MRP-14

Entrez Gene IDs

6280 (Human); 20202 (Mouse)

Gene Symbol

S100A9

UniProt

P31725

Additional S100A9 Products

Product Documents for Mouse S100A9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse S100A9 Antibody

For research use only

Citations for Mouse S100A9 Antibody

Customer Reviews for Mouse S100A9 Antibody (5)

4.6 out of 5
5 Customer Ratings
5 Stars
60%
4 Stars
40%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Mouse S100A9 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 5 of 5 reviews Showing All
Filter By:
Clear Filters X
  • Mouse S100A9 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: A549 human lung carcinoma cell line
    Species: Human
    Verified Customer | Posted 05/24/2022
    Mouse S100A9 Antibody AF2065
  • Mouse S100A9 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Adult brain
    Species: Human
    Verified Customer | Posted 04/04/2022
    Mouse S100A9 Antibody AF2065
  • Mouse S100A9 Antibody
    Name: Gonzalo Gonzalez
    Application: Western Blot
    Sample Tested: mouse feces
    Species: Mouse
    Verified Customer | Posted 05/03/2017
    S100A9 detection in mouse feces at 1:500, 1:1000 and 1:2000 dilution. Incubation time was 2 hrs at RT. Antibody in 1% milk TBST
    Mouse S100A9 Antibody AF2065
  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: See PMID 21228116
    Species: Mouse
    Verified Customer | Posted 01/06/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23481567
    Species: Mouse
    Verified Customer | Posted 01/06/2015

There are no reviews that match your criteria.

Showing  1 - 5 of 5 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies