Mouse TNF-alpha Antibody

(51 citations)
(3 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TNF-alpha  in ELISAs and Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant rat TNF‑ alpha is observed and less than 1% cross-reactivity with recombinant human TNF-alpha is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse TNF-alpha (R&D Systems, Catalog # 410-MT)
    Leu80-Leu235
    Accession # P06804
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse TNF‑ alpha (Catalog # 410-MT)
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    RAW 264.7 mouse monocyte/macrophage cell line treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin
    • Mouse TNF-alpha Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse TNF‑ alpha Antibody (Catalog # AF-410-NA)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse TNF‑ alpha Biotinylated Antibody (Catalog # BAF410)
  • ELISA Standard
     
    Recombinant Mouse TNF-alpha (aa 80-235) Protein (Catalog # 410-MT)
  • Neutralization
    Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 0.25 ng/mL Recombinant Mouse TNF‑ alpha and 1 µg/mL actinomycin D.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
TNF‑ alpha in Mouse T Cells. TNF‑ alpha was detected in immersion fixed activated mouse T Cells using 15 µg/mL Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑410‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Cytotoxicity Induced by TNF‑ alpha and Neutralization by Mouse TNF‑ alpha Antibody. Recombinant Mouse TNF‑ alpha (Catalog # 410-MT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF‑ alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA). The ND50 is typically 0.1-0.4 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
Immunocytochemistry
TNF‑ alpha in RAW 264.7 Mouse Cell Line. TNF‑ alpha was detected in immersion fixed RAW 264.7 mouse monocyte/ macrophage cell line treated with LPS using Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF-alpha

Tumor necrosis factor alpha (TNF-alpha, also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types. Mouse TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, mouse TNF-alpha shares 94% aa sequence identity with rat and 70% - 77% with bovine, canine, cotton rat, equine, feline, human, porcine, rat, and rhesus TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness. Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha. TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-alpha regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-alpha is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.

  • Long Name:
    Tumor Necrosis Factor alpha
  • Entrez Gene IDs:
    7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
  • Alternate Names:
    APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

51 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Gender difference in NASH susceptibility: Roles of hepatocyte Ikk? and Sult1e1
    Authors: N Matsushita, MT Hassanein, M Martinez-C, R Lazaro, SW French, W Xie, K Lai, M Karin, H Tsukamoto
    PLoS ONE, 2017;12(8):e0181052.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC paraffin embedded
  2. A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity
    Authors: KJ Chung, A Chatzigeor, M Economopou, R Garcia-Mar, VI Alexaki, I Mitroulis, M Nati, J Gebler, T Ziemssen, SE Goelz, J Phieler, JH Lim, KP Karalis, T Papayannop, M Blüher, G Hajishenga, T Chavakis
    Nat. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  3. Deficiency of PTP1B Attenuates Hypothalamic Inflammation via Activation of the JAK2-STAT3 Pathway in Microglia
    Authors: T Tsunekawa, R Banno, A Mizoguchi, M Sugiyama, T Tominaga, T Onoue, D Hagiwara, Y Ito, S Iwama, M Goto, H Suga, Y Sugimura, H Arima
    EBioMedicine, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC- Frozen
  4. Tumor-infiltrating monocytes/macrophages promote tumor invasion and migration by upregulating S100A8 and S100A9 expression in cancer cells
    Authors: SY Lim, AE Yuzhalin, AN Gordon-Wee, RJ Muschel
    Oncogene, 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: Neut
  5. Glimepiride reduces CD14 expression and cytokine secretion from macrophages.
    Authors: Ingham V, Williams A, Bate C
    J Neuroinflammation, 2014;11(0):115.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Capture
  6. No effect of ablation of surfactant protein-D on acute cerebral infarction in mice.
    Authors: Lambertsen K, Ostergaard K, Clausen B, Hansen S, Stenvang J, Thorsen S, Meldgaard M, Kristensen B, Hansen P, Sorensen G, Finsen B
    J Neuroinflammation, 2014;11(0):123.
    Species: Mouse
    Sample Type: Plasma
    Application: Functional Assay
  7. Pathophysiology of lung injury induced by common bile duct ligation in mice.
    Authors: Shikata F, Sakaue T, Nakashiro K, Okazaki M, Kurata M, Okamura T, Okura M, Ryugo M, Nakamura Y, Yasugi T, Higashiyama S, Izutani H
    PLoS ONE, 2014;9(4):e94550.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. FcgammaRIIb inhibits immune complex-induced VEGF-A production and intranodal lymphangiogenesis.
    Authors: Clatworthy M, Harford S, Mathews R, Smith K
    Proc Natl Acad Sci U S A, 2014;111(50):17971-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  9. Cytokine conditioning enhances systemic delivery and therapy of an oncolytic virus.
    Authors: Ilett E, Kottke T, Donnelly O, Thompson J, Willmon C, Diaz R, Zaidi S, Coffey M, Selby P, Harrington K, Pandha H, Melcher A, Vile R
    Mol Ther, 2014;22(10):1851-63.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  10. Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.
    Authors: Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K
    Nat Med, 2014;20(4):377-84.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
    Authors: Junkins R, MacNeil A, Wu Z, McCormick C, Lin T
    J Immunol, 2013;190(10):5178-86.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Capture
  12. Mice lacking C1q are protected from high fat diet-induced hepatic insulin resistance and impaired glucose homeostasis.
    Authors: Hillian, Antoinet, McMullen, Megan R, Sebastian, Becky M, Roychowdhury, Sanjoy, Kashyap, Sangeeta, Schauer, Philip R, Kirwan, John P, Feldstein, Ariel E, Nagy, Laura E
    J Biol Chem, 2013;288(31):22565-75.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  13. Wingless-type mammary tumor virus integration site family, member 5A (Wnt5a) regulates human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein 120 (gp120)-induced expression of pro-inflammatory cytokines via the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and c-Jun N-terminal kinase (JNK) signaling pathways.
    Authors: Li B, Shi Y, Shu J, Gao J, Wu P, Tang S
    J Biol Chem, 2013;288(19):13610-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  14. Defects in the peripheral taste structure and function in the MRL/lpr mouse model of autoimmune disease.
    Authors: Kim A, Feng P, Ohkuri T, Sauers D, Cohn ZJ, Chai J, Nelson T, Bachmanov AA, Huang L, Wang H
    PLoS ONE, 2012;7(4):e35588.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  15. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Type: Plasma
    Application: Luminex Assay Development
  16. IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells.
    Authors: Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS
    J. Clin. Invest., 2011;121(10):3846-59.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  17. Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model.
    Authors: Wendt W, Schulten R, Stichel CC, Lubbert H
    J. Neurochem., 2009;110(6):1931-41.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  18. Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9.
    Authors: Koronyo-Hamaoui M, Ko MK, Koronyo Y, Azoulay D, Seksenyan A, Kunis G, Pham M, Bakhsheshian J, Rogeri P, Black KL, Farkas DL, Schwartz M
    J. Neurochem., 2009;111(6):1409-24.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  19. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
    Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
    J. Immunol., 2009;183(9):5799-806.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  20. Cooperative phagocytes: resident microglia and bone marrow immigrants remove dead photoreceptors in retinal lesions.
    Authors: Joly S, Francke M, Ulbricht E, Beck S, Seeliger M, Hirrlinger P, Hirrlinger J, Lang KS, Zinkernagel M, Odermatt B, Samardzija M, Reichenbach A, Grimm C, Reme CE
    Am. J. Pathol., 2009;174(6):2310-23.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  21. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
    Microbes Infect., 2009;11(4):524-30.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  22. Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS).
    Authors: Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA
    J. Biol. Chem., 2009;0(0):.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  23. TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
    Authors: Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM
    J. Clin. Invest., 2009;119(10):2954-64.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  24. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
    Immunology, 2008;125(2):272-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  25. Rod-Shaped monocytes patrol the brain vasculature and give rise to perivascular macrophages under the influence of proinflammatory cytokines and angiopoietin-2.
    Authors: Audoy-Remus J, Richard JF, Soulet D, Zhou H, Kubes P, Vallieres L
    J. Neurosci., 2008;28(41):10187-99.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  26. CT20126, a novel immunosuppressant, prevents collagen-induced arthritis through the down-regulation of inflammatory gene expression by inhibiting NF-kappaB activation.
    Authors: Lee SJ, Nam WD, Na HJ, Cho YL, Ha KS, Hwang JY, Lee H, Kim SO, Kwon YG, Kim YM
    Biochem. Pharmacol., 2008;76(1):79-90.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  27. Hypoxia enhances lysosomal TNF-alpha degradation in mouse peritoneal macrophages.
    Authors: Lahat N, Rahat MA, Kinarty A, Weiss-Cerem L, Pinchevski S, Bitterman H
    Am. J. Physiol., Cell Physiol., 2008;295(1):C2-12.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  28. Tumor-specific Th17-polarized cells eradicate large established melanoma.
    Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
    Blood, 2008;112(2):362-73.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  29. Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice.
    Authors: Kim HG, Kim NR, Gim MG, Lee JM, Lee SY, Ko MY, Kim JY, Han SH, Chung DK
    J. Immunol., 2008;180(4):2553-61.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Development
  30. Antipsoriatic effects of avarol-3'-thiosalicylate are mediated by inhibition of TNF-alpha generation and NF-kappaB activation in mouse skin.
    Authors: Amigo M, Paya M, De Rosa S, Terencio MC
    Br. J. Pharmacol., 2007;152(3):353-65.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  31. TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor.
    Authors: Cunha TM, Verri WA, Jr, Fukada SY, Guerrero AT, Santodomingo-Garzon T, Poole S, Parada CA, Ferreira SH, Cunha FQ
    Eur. J. Pharmacol., 2007;573(1):221-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  32. Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
    Authors: Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
    Eur. J. Immunol., 2006;36(8):2025-34.
    Species: Mouse
    Sample Type: Tissue Secretion
    Application: ELISA Development
  33. Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
    Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
    Infect. Immun., 2006;74(12):6730-8.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development
  34. Protective effects of neurotrophic factors on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of murine adrenal chromaffin cell line tsAM5D.
    Authors: Murata T, Tsuboi M, Hikita K, Kaneda N
    J. Biol. Chem., 2006;281(32):22503-16.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  35. Glatiramer acetate fights against Alzheimer's disease by inducing dendritic-like microglia expressing insulin-like growth factor 1.
    Authors: Butovsky O, Koronyo-Hamaoui M, Kunis G, Ophir E, Landa G, Cohen H, Schwartz M
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(31):11784-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  36. Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages.
    Authors: An HJ, Jeong HJ, Um JY, Kim HM, Hong SH
    J Ethnopharmacol, 2006;106(3):418-24.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  37. Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
    Authors: Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
    J. Immunol., 2006;177(1):612-20.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development
  38. Microglia activated by IL-4 or IFN-gamma differentially induce neurogenesis and oligodendrogenesis from adult stem/progenitor cells.
    Authors: Butovsky O, Ziv Y, Schwartz A, Landa G, Talpalar AE, Pluchino S, Martino G, Schwartz M
    Mol. Cell. Neurosci., 2006;31(1):149-60.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  39. Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice.
    Authors: Penido C, Conte FP, Chagas MS, Rodrigues CA, Pereira JF, Henriques MG
    Inflamm. Res., 2006;55(11):457-64.
    Species: Mouse
    Sample Type: Synovial Fluid
    Application: ELISA Development
  40. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006;177(3):1967-74.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  41. Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
    Authors: Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A
    J. Immunol., 2006;177(12):8650-7.
    Species: Mouse
    Sample Type: Peritoneal Lavage
    Application: ELISA Development
  42. T lineage differentiation from human embryonic stem cells.
    Authors: Galic Z, Kitchen SG, Kacena A, Subramanian A, Burke B, Cortado R, Zack JA
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(31):11742-7.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  43. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005;302(1):172-81.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  44. The sex steroid precursor DHEA accelerates cutaneous wound healing via the estrogen receptors.
    Authors: Mills SJ, Ashworth JJ, Gilliver SC, Hardman MJ, Ashcroft GS
    J. Invest. Dermatol., 2005;125(5):1053-62.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  45. Tumor necrosis factor-like weak inducer of apoptosis increases the permeability of the neurovascular unit through nuclear factor-kappa B pathway activation.
    Authors: Polavarapu R, Gongora MC, Winkles JA, Yepes M
    J. Neurosci., 2005;25(44):10094-100.
    Species: Mouse
    Sample Type:
    Application: In vivo
  46. Antibody-bound beta-amyloid precursor protein stimulates the production of tumor necrosis factor-alpha and monocyte chemoattractant protein-1 by cortical neurons.
    Authors: Gonzalez de Aguilar JL, Dupuis L, Larmet Y
    Neurobiol. Dis., 2005;19(1):129-41.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  47. Selective macrophage suppression during sepsis.
    Authors: Ellaban E, Bolgos G, Remick D
    Cell. Immunol., 2004;231(1):103-11.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  48. Role of tumor necrosis factor receptor-1 in the death of retinal ganglion cells following optic nerve crush injury in mice.
    Authors: Tezel G, Yang X, Yang J, Wax MB
    Brain Res., 2004;996(2):202-12.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  49. Transcriptional profiling of lipopolysaccharide-induced acute lung injury.
    Authors: Jeyaseelan S, Chu HW, Young SK, Worthen GS
    Infect. Immun., 2004;72(12):7247-56.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  50. Thyrocytes isolated from autoimmune-diseased thyroids secrete soluble tumor necrosis factor-R1 that is related to their elevated protein kinase C activity.
    Authors: LaBue M, Colburn KK, Green LM
    Thyroid, 2004;14(4):249-62.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: IP
  51. Elevated levels of tumor necrosis factor alpha (TNF-alpha) in human immunodeficiency virus type 1-transgenic mice: prevention of death by antibody to TNF-alpha.
    Authors: De SK, Devadas K, Notkins AL
    J. Virol., 2002;76(22):11710-4.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
Expand to show all 51 Citations
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 4.7 (Based on 3 reviews)

Have you used Mouse TNF-alpha Antibody?

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Read what people are saying who have used Mouse TNF-alpha Antibody.

We have 3 reviews tested in 1 species: Mouse.
We have 3 reviews tested in 2 applications: ELISA, Block/Neutralize.

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Images Ratings Applications Species Reviewed By Date Details
ELISA Mouse TNF-alpha  Antibody AF-410-NA
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Very Good
 ELISA Mouse Anonymous 12/01/2017
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ELISA Mouse TNF-alpha  Antibody AF-410-NA
ELISA: Mouse TNF-alpha Antibody [AF-410-NA]

Summary

ApplicationELISA
Sample TestedRecombinant protein
SpeciesMouse
  Excellent
 ELISA Mouse Anonymous 10/30/2017
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Summary

ApplicationELISA
Sample TestedLiver tissue
SpeciesMouse
  Excellent
 Block/Neutralize Mouse Anonymous 05/10/2017
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Summary

ApplicationBlock/Neutralize
Sample TestedRAW 264.7 mouse monocyte/macrophage cell line
SpeciesMouse

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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