Mouse TSLP R Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TSLP R in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse TSLP R
    Ala20-Leu233
    Accession # BAA92159
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse TSLP R Fc Chimera (Catalog # 546-TR)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize TSLP-induced proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 R alpha /CD127. Park, L.S. et al. (2000) J. Exp. Med. 192:659. The Neutralization Dose (ND50) is typically 0.2-0.6 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse TSLP.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TSLP R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF546, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP R Antibody. Recombinant Mouse TSLP (Catalog # 555-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 R alpha /CD127 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF546). The ND50 is typically 0.2-0.6 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TSLP R

TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The TSLP R cDNA encodes a 359 amino acid (aa) residue type 1 membrane protein with a 24 aa residue signal peptide, a 206 aa residue extracellular domain that showed 24% sequence identity with the mouse common gamma receptor, a 23 aa residue transmembrane domain, and a 106 aa residue cytoplasmic domain. The cytoplasmic domain of TSLP R contains a membrane-proximal box1 motif which is known to be important for association with JAKs. An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3, 4).

  • References:
    1. Fujio, K. et al. (2000) Blood 95:2204.
    2. Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
    3. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
    4. Pandey, A. et al. (2000) Nat. Immunol. 1:59.
  • Long Name:
    Thymic Stromal Lymphopoietin Receptor
  • Entrez Gene IDs:
    64109 (Human); 57914 (Mouse); 171499 (Rat)
  • Alternate Names:
    CRL2; CRL2cytokine receptor CRL2 precusor; CRLF2; CRLF2Y; CRLM-2; Cytokine receptor-like 2; cytokine receptor-like factor 2; ILXR; IL-XR; P2RY8/CRLF2 fusion; PCOR1; Thymic stromal lymphopoietin protein receptor; thymic stromal lymphopoietin receptor; thymic stromal-derived lymphopoietin receptor; TSLP R; TSLP receptor; TSLPR
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. IL-7R? Expression Regulates Murine Dendritic Cell Sensitivity to Thymic Stromal Lymphopoietin
    Authors: L Kummola, Z Ortutay, X Chen, S Caucheteux, S Hämäläinen, S Aittomäki, R Yagi, J Zhu, M Pesu, WE Paul, IS Junttila
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Rearrangement of CRLF2 in B-progenitor- and Down syndrome-associated acute lymphoblastic leukemia.
    Authors: Mullighan CG, Collins-Underwood JR, Phillips LA, Loudin MG, Liu W, Zhang J, Ma J, Coustan-Smith E, Harvey RC, Willman CL, Mikhail FM, Meyer J, Carroll AJ, Williams RT, Cheng J, Heerema NA, Basso G, Pession A, Pui CH, Raimondi SC, Hunger SP, Downing JR, Carroll WL, Rabin KR
    Nat. Genet., 2009;41(11):1243-6.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. Overexpression of murine TSLP impairs lymphopoiesis and myelopoiesis.
    Authors: Osborn MJ, Ryan PL, Kirchhof N, Panoskaltsis-Mortari A, Mortari F, Tudor KS
    Blood, 2004;103(3):843-51.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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