Mouse TWEAK R/TNFRSF12 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TWEAK R in ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant human TWEAK R is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse TWEAK R/TNFRSF12
    Glu28-Trp79
    Accession # Q9CR75
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse TWEAK R/TNFRSF12 Fc Chimera (Catalog # 1610-TW)
  • Flow Cytometry
    2.5 µg/106 cells
    MS‑1 mouse pancreatic islet endothelial cell line
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
    • Mouse TWEAK R/TNFRSF12 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse TWEAK R/TNFRSF12 Antibody (Catalog # AF1610)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse TWEAK R/TNFRSF12 Biotinylated Antibody (Catalog # BAF1610)
  • ELISA Standard
     
    Recombinant Mouse TWEAK R/TNFRSF12 Fc Chimera Protein, CF (Catalog # 1610-TW)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
TWEAK R/TNFRSF12 in Mouse Kidney. TWEAK R/TNFRSF12 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse TWEAK R/TNFRSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1610) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TWEAK R/TNFRSF12

TNF-related weak inducer of apoptosis receptor (TWEAK R) belongs to the TNF receptor superfamily and is designated TNFRSF12. The gene for TWEAK R was originally identified as a fibroblast growth factor-inducible immediate-early response gene Fn14 in mouse NIH 3T3 fibroblasts (1, 2). Mouse TWEAK R cDNA encodes a 129 amino acid (aa) residues type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain, and a 28 aa cytoplasmic domain (1-3). Human and mouse TWEAK R share 82% aa sequence identity. TWEAK R is the smallest member of the TNF receptor superfamily and contains only one cysteine-rich region in its extracellular domain. The TWEAK R cytoplasmic domain contains one TRAF binding motif which binds TRAFs 1, 2, and 3. TWEAK R binds its ligand TWEAK/TNFSF12 with high affinity to initiate a signal transduction cascade that depending upon the cell type, may lead to a variety of cellular responses including cell death by both caspase-dependent apoptosis and cathepsin B-dependent necrosis, cell proliferation, and angiogenesis (2-6). In newborn mice, TWEAK R is highly expressed in all tissues examined (heart, intestine, kidney, liver, lung, and skin) (1). In adult mice, high TWEAK R expression levels are found in the heart and ovary, while lower expression levels are detected in the lung, kidney, skin. Elevated levels of TWEAK R mRNA were found in human or mouse hepatocellular carcinoma specimens, in regenerating mouse liver and in injured rat arteries (2, 3).

  • References:
    1. Meighan-Mantha, R. et al. (1999) J. Biol. Chem. 274:33166.
    2. Feng, S. et al. (2000) Am. J. Pathol. 156:1253.
    3. Wiley, S. et al. (2001) Immunity 15:837.
    4. Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.
    5. Nakayama, M. et al. (2002) J. Immunol. 168:734.
    6. Lynch, C.N. et al. (1999) J. Biol. Chem. 274:8455.
  • Long Name:
    TNF-like and Weak Inducer of Apoptosis Receptor
  • Entrez Gene IDs:
    51330 (Human); 27279 (Mouse)
  • Alternate Names:
    CD266 antigen; CD266; FGF-inducible 14; Fibroblast growth factor-inducible immediate-early response protein 14; Fn14; FN14tumor necrosis factor receptor superfamily member 12A; TNFRSF12; TNFRSF12A; tumor necrosis factor receptor superfamily, member 12A; TWEAK R; TWEAKR; tweak-receptor; type I transmembrane protein Fn14
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