Porcine IL-1ra/IL-1F3 Antibody
Porcine IL-1ra/IL-1F3 Antibody Summary
Accession # Q29056
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IL‑1ra/IL‑1F3 Inhibition of IL‑1 alpha /IL‑1F1-dependent Cell Proliferation and Neutral-ization by Porcine IL‑1ra/ IL‑1F3 Antibody. Recombinant Porcine IL-1ra/ IL-1F3 (Catalog # 780-RA) inhibits Recombinant Porcine IL-1a/IL-1F1 (Catalog # 680-PI) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Porcine IL-1a/ IL-1F1 (75 pg/mL) activity elicited by Recombinant Porcine IL-1ra/ IL-1F3 (0.2 µg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Porcine IL-1ra/IL-1F3 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF780). The ND50 is typically 3-15 µg/mL.
IL‑1ra/IL‑1F3 in Porcine PBMCs. IL-1ra/IL-1F3 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Porcine IL-1ra/ IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF780) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally-occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptors (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Human, mouse and rat IL-1ra all share approximately 80% homology with porcine IL-1ra.
Citation for Porcine IL-1ra/IL-1F3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Induction of interleukin-1 in articular cartilage by explantation and cutting.
Authors: Gruber J, Vincent TL, Hermansson M, Bolton M, Wait R, Saklatvala J
Arthritis Rheum., 2004-08-01;50(8):2539-46.
Sample Types: Whole Cells
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