|Detection of Rat Adiponectin/Acrp30 by Western Blot. Western blot shows lysates of rat adipose tissue and rat mammary gland tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Rat Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB3100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Adiponectin/Acrp30 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|Adiponectin/Acrp30 in Rat Adipocytes. Adiponectin/Acrp30 was detected in Immersion fixed rat mesenchymal stem cells differentiated into adipocytes with Human/Mouse/Rat StemXVivo Adipogenic Supplement (Catalog # CCM011) using Mouse Anti-Rat Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB3100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
Adiponectin, also known as Adipocyte complement-related protein of 30 kDa (Acrp30), is a secreted adipose tissue-specific protein. It is an anti-atherogenic, anti-diabetic and anti-inflammatory hormone that plays important roles in the regulation of lipid and glucose metabolism. Rat Adiponectin/Acrp30 shares 91% and 83% amino acid sequence homology with mouse and human Adiponectin/Acrp30, respectively.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided