Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Western Blot, Neutralization

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 251021
View all CXCL1/GRO alpha/KC/CINC-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat CXCL1/GRO alpha /KC/CINC‑1
Ala25-Lys96
Accession # P14095

Specificity

Detects rat CXCL1/GRO alpha /KC/CINC‑1 in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant human (rh) GRO alpha, rhGRO beta, rhGRO gamma, and recombinant mouse KC is observed. No cross-reactivity with recombinant rat (rr) CINC2a, rrCXCL3, rhCXCL5, 6, 7, 8, 9, 10, 11, 12 or 13, rmCXCL2, 6, 9, 10, 12, 13, or recombinant porcine CXCL8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Rat CXCL1/GRO alpha /KC/CINC‑1 Antibody

Chemotaxis Induced by CXCL1/CINC‑1 and Neutral-ization by Rat CXCL1/ CINC‑1 Antibody.

Chemotaxis Induced by CXCL1/CINC‑1 and Neutral-ization by Rat CXCL1/ CINC‑1 Antibody.

Recombinant Rat CXCL1/CINC-1 (Catalog # 515-CN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL1/ CINC-1 (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CXCL1/CINC-1 Monoclonal Antibody (Catalog # MAB515). The ND50 is typically 3-15 µg/mL.

Applications for Rat CXCL1/GRO alpha /KC/CINC‑1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Rat CXCL1/GRO alpha /KC/CINC‑1 (Catalog # 515-CN)

Neutralization

Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 3-15 µg/mL in the presence of 4 ng/mL Recombinant Rat CXCL1/GRO alpha /KC/CINC‑1.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL1/GRO alpha/KC/CINC-1

Cytokine-induced neutrophil chemoattractant 1 (CINC-1) was originally purified from media conditioned by IL-1 beta stimulated rat kidney epithelioid cells (NRK-52E). On the basis of its protein and DNA sequences, CINC-1 is a member of the alpha (CXC) subfamily of chemokines, designated CXCL1. Three additional rat CXC chemokines (CINC-2 alpha, CINC-2 beta, CINC-3/MIP-2), sharing approximately 63-67% amino acid sequence identity with CINC-1, have been identified. The protein sequence of rat CINC-1 is also 68%, 71%, and 69% identical to that of human GRO-alpha, GRO-beta, and GRO-gamma, respectively. Based on their sequence homology, it has been suggested that CINCs are the rat counterpart of human GROs. CINC-1 is also the counterpart of mouse KC. Rat CINC-1 cDNA encodes a 96 amino acid residue precursor protein from which the amino-terminal 24 amino acid residues are cleaved to generate the mature CINC-1. Similar to other alpha chemokines, rat CINCs are potent neutrophil attractants and activators and have been shown to play an important role in the infiltration of neutrophils into inflammatory sites in rats.

Alternate Names

CINC-1, CINC1, GRO alpha, KC, MGSA-alpha

Entrez Gene IDs

2919 (Human); 14825 (Mouse); 81503 (Rat)

Gene Symbol

CXCL1

UniProt

P14095

Additional CXCL1/GRO alpha/KC/CINC-1 Products

Product Documents for Rat CXCL1/GRO alpha /KC/CINC‑1 Antibody

Certificate of Analysis

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Product Specific Notices for Rat CXCL1/GRO alpha /KC/CINC‑1 Antibody

For research use only

Citations for Rat CXCL1/GRO alpha /KC/CINC‑1 Antibody

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