Rat CXCL3/CINC-2 alpha / beta Antibody Summary
Accession # Q10746
Rat CXCL3/CINC-2 alpha / beta Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CXCL3/CINC‑2 alpha and Neutralization by Rat CXCL3/CINC‑2 alpha / beta Antibody. Recombinant Rat CXCL3/ CINC-2a (Catalog # 516-CA) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL3/ CINC-2a (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Rat CXCL3/CINC-2a/ beta Monoclonal Antibody (Catalog # MAB516). The ND50is typically 0.5-2.5 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL3/GRO gamma/CINC-2/DCIP-1
CINC-2 alpha and -2 beta are mRNA splice variant isoforms that differ by only 3 amino acids at their amino-termini. These proteins are CXC chemokines most closely related to human GROs and mouse MIP-2. They are produced by granulation tissue, activated macrophages and fibroblasts. CINC-2 biological activities are mediated by CXCR1 or CXCR2.
Citation for Rat CXCL3/CINC-2 alpha / beta Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines.
Authors: Miura TA, Wang J, Holmes KV, Mason RJ
Sample Types: Cell Culture Supernates
Applications: ELISA Development
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