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Rat CXCL3/CINC-2 alpha / beta Antibody

Catalog #: MAB516
1 Citation Datasheet / COA / SDS
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MAB516
MAB516-SP

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Chemotaxis Induced by CXCL3/CINC‑2 alpha and Neutralization by Rat CXCL3/CINC‑2 alpha / beta Antibody.
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Rat CXCL3/CINC-2 alpha / beta Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat CXCL3/CINC-2 alpha / beta in ELISAs and Western blots. In sandwich immunoassays, 27.9% cross‑reactivity with recombinant rat (rr) CINC-3 is observed and no cross-reactivity or interference with rrCINC‑1, recombinant mouse (rm) KC, rmMIP-2,  or recombinant human GRO alpha is observed.
Source
Monoclonal Mouse IgG2B Clone # 83504
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant rat CXCL3/CINC-2 alpha / beta
Arg33-Ser100
Accession # Q10746
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Rat CXCL3/CINC-2 alpha (Catalog # 516-CA)
Recombinant Rat CXCL3/CINC-2 beta (Catalog # 517-CB)

Rat CXCL3/CINC-2 alpha / beta Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Rat CXCL3/GRO gamma/CINC-2/DCIP-1 Biotinylated Antibody (Catalog # BAF516)

Standard: Recombinant Rat CXCL3/CINC-2 beta Protein (Catalog # 517-CB)

Standard: Recombinant Rat CXCL3/CINC-2 alpha Protein (Catalog # 516-CA)

Neutralization
Measured by its ability to neutralize CXCL3/CINC‑2 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 0.05 µg/mL Recombinant Rat CXCL3/CINC‑2 alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CXCL3/CINC‑2 alpha and Neutralization by Rat CXCL3/CINC‑2 alpha / beta Antibody. View Larger

Chemotaxis Induced by CXCL3/CINC‑2 alpha and Neutralization by Rat CXCL3/CINC‑2 alpha / beta Antibody. Recombinant Rat CXCL3/ CINC-2a (Catalog # 516-CA) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL3/ CINC-2a (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Rat CXCL3/CINC-2a/ beta Monoclonal Antibody (Catalog # MAB516). The ND50 is typically 0.5-2.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL3/GRO gamma/CINC-2/DCIP-1

CINC-2 alpha and -2 beta are mRNA splice variant isoforms that differ by only 3 amino acids at their amino-termini. These proteins are CXC chemokines most closely related to human GROs and mouse MIP-2. They are produced by granulation tissue, activated macrophages and fibroblasts. CINC-2 biological activities are mediated by CXCR1 or CXCR2.

Entrez Gene IDs
2921 (Human); 330122 (Mouse); 171551 (Rat)
Alternate Names
chemokine (C-X-C motif) ligand 3; CINC-2; CINC-2b; C-X-C motif chemokine 3; CXCL3; Dcip1; Gm1960; GRO gamma; GRO3 oncogene; GRO3; GROg; GRO-gamma; GRO-gamma(1-73); Growth-regulated protein gamma; Macrophage inflammatory protein 2-beta; melanoma growth stimulatory activity gamma; MGSA gamma; MIP2B; MIP-2b; MIP2-beta; SCYB3

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Citation for Rat CXCL3/CINC-2 alpha / beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines.
    Authors: Miura TA, Wang J, Holmes KV, Mason RJ
    Virology, 2007-09-05;369(2):288-98.
    Species: Rat
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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