Rat CXCL3/CINC-2 alpha / beta Antibody

(1 citations)   
  • Species Reactivity
    Rat
  • Specificity
    Detects rat CXCL3/CINC-2 alpha / beta in ELISAs and Western blots. In sandwich immunoassays, 27.9% cross‑reactivity with recombinant rat (rr) CINC-3 is observed and no cross-reactivity or interference with rrCINC‑1, recombinant mouse (rm) KC, rmMIP-2,  or recombinant human GRO alpha is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 83504
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant rat CXCL3/CINC-2 alpha / beta
    Arg33-Ser100
    Accession # Q10746
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Rat CXCL3/CINC-2 alpha (Catalog # 516-CA)
    Recombinant Rat CXCL3/CINC-2 beta (Catalog # 517-CB)
    • Rat CXCL3/CINC-2 alpha / beta Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Rat CXCL3/CINC-2 alpha / beta Antibody (Catalog # MAB516)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Rat CXCL3/GRO gamma/CINC-2/DCIP-1 Biotinylated Antibody (Catalog # BAF516)
  • ELISA Standard
     
    Recombinant Rat CXCL3/CINC-2 alpha Protein (Catalog # 516-CA)
  • ELISA Standard
     
    Recombinant Rat CXCL3/CINC-2 beta Protein (Catalog # 517-CB)
  • Neutralization
    Measured by its ability to neutralize CXCL3/CINC‑2 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 0.05 µg/mL Recombinant Rat CXCL3/CINC‑2 alpha.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CXCL3/CINC‑2 alpha and Neutralization by Rat
CXCL3/CINC‑2 alpha / beta  Antibody.

Recombinant Rat CXCL3/
CINC‑2 alpha (Catalog # 516-CA) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL3/
CINC‑2 alpha (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Rat CXCL3/CINC‑2 alpha / beta Monoclonal Antibody (Catalog # MAB516). The ND50 is typically
0.5‑2.5 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL3/GRO gamma/CINC-2/DCIP-1
CINC-2 alpha and -2 beta are mRNA splice variant isoforms that differ by only 3 amino acids at their amino-termini. These proteins are CXC chemokines most closely related to human GROs and mouse MIP-2. They are produced by granulation tissue, activated macrophages and fibroblasts. CINC-2 biological activities are mediated by CXCR1 or CXCR2.
  • Entrez Gene IDs:
    2921 (Human); 330122 (Mouse); 171551 (Rat)
  • Alternate Names:
    chemokine (C-X-C motif) ligand 3; CINC-2; CINC-2b; C-X-C motif chemokine 3; CXCL3; Dcip1; Gm1960; GRO gamma; GRO3 oncogene; GRO3; GROG; GRO-gamma; GRO-gamma(1-73); Growth-regulated protein gamma; Macrophage inflammatory protein 2-beta; melanoma growth stimulatory activity gamma; MGSA gamma; MIP2B; MIP-2b; MIP2-beta; SCYB3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines.
    Authors: Miura TA, Wang J, Holmes KV, Mason RJ
    Virology, 2007;369(2):288-98.
    Species: Rat
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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