Rat GFR alpha-1/GDNF R alpha-1 Antibody Summary
Accession # Q62997
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Rat GFR alpha ‑1/GDNF R alpha ‑1 by Western Blot. Western blot shows lysates of rat spinal cord tissue and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat GFRa-1/GDNF Ra-1 Monoclonal Antibody (Catalog # MAB560) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GFRa-1/GDNF Ra-1 at approximately 60 kDa (as indicated). This experiment was conducted under non-reducing conditions (recommended) and using Immunoblot Buffer Group 1.
GFR alpha ‑1/GDNF R alpha ‑1 in Rat Spinal Cord. GFRa-1/GDNF Ra-1 was detected in perfusion fixed frozen sections of rat spinal cord using Rat GFRa-1/GDNF Ra-1 Monoclonal Antibody (Catalog # MAB560) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GFR alpha-1/GDNF R alpha-1
Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF-beta superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha - (GFR alpha -1) or GFR alpha -2). GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase. Rat GFR alpha -1 cDNA encodes a 468 amino acid (aa) residue protein with an N‑terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% amino acid identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.
- Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
- Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
- Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
Citations for Rat GFR alpha-1/GDNF R alpha-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM).
Authors: Euteneuer S, Yang K, Chavez E, Leichtle A, Loers G, Olshansky A, Pak K, Schachner M, Ryan A
Mol Cell Neurosci, 2013;54(0):30-43.
Sample Types: Whole Tissue
Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
Authors: Kubota H, Wu X, Goodyear SM, Avarbock MR, Brinster RL
FASEB J., 2011;25(0):2604-14.
Sample Types: Whole Cells
Applications: Flow Cytometry
Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin.
Authors: Carmillo P, Dago L, Day ES, Worley DS, Rossomando A, Walus L, Orozco O, Buckley C, Miller S, Tse A, Cate RL, Rosenblad C, Sah DW, Gronborg M, Whitty A
Sample Types: Whole Cells
Expression and function of GDNF family ligands and receptors in the carotid body.
Authors: Leitner ML, Wang LH, Osborne PA, Golden JP, Milbrandt J, Johnson EM
Exp. Neurol., 2005;191(0):S68-79.
Sample Types: Whole Cells
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