Rat UNC5H2/UNC5B Antibody AF1006: R&D Systems

Rat UNC5H2/UNC5B Antibody

(8 citations)   
  • Species Reactivity
    Rat
  • Specificity
    Detects rat UNC5H2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) UNC5H3 and recombinant rat UNC5H1 is observed, and less than 1% cross-reactivity with rhUNC5H4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat UNC5H2
    Gly27-Asp373
    Accession # O08722
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed frozen sections of rat brain
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rat UNC5H2 by Western Blot. Western blot shows lysates of rat spinal cord tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Rat UNC5H2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1006) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for UNC5H2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: UNC5H2/UNC5B

Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues (including rat UNC5H1 and H2, mouse UNC5H2 and H3 (also known as rostral cerebellar malformation, RCM), and human UNC5H3 and H4) are transmembrane proteins belonging to the immunoglobulin (Ig) superfamily. All UNC5 family members have two Ig and two thrombospondin type 1 domains in their extracellular regions, as well as a conserved ZU-5 domain, a DCC (Deleted in Colorectal Cancer)-binding domain (DB) and a C-terminal death domain (DD) in their cytoplasmic regions (1, 2).

UNC5 family proteins are receptors for the netrin/UNC6 (netr: Sanskrit for “one who guides”) family of secreted axon guidance cues that are laminin-related proteins. Netrin family proteins can act as a chemoattractant for some axons and as a chemorepellent for others. Besides UNC5, netrin family proteins also bind to the DCC family of type I transmembrane receptors that share sequence similarity with proteins of the NCAM family, and adenosine A2b receptor, a G protein-coupled seven‑transmembrane receptor belonging to the adenosine receptor family (3, 4). In vitro, netrin binding to DCC family receptors in the absence of UNC5 is associated with axon attraction. However, the DCC-mediated attraction to netrin is converted to repulsion by binding of UNC5 to the DCC-netrin complex. In vivo, the mechanisms of netrin-dependent axon attraction and repulsion are more complex and may include UNC5-mediated repulsion that is independent of DCC (1, 5). Besides their roles in axon guidance and neuronal migration, the UNC5 and DCC families also act as dependence receptors and exert pro-apoptotic effects in the absence of netrin (6).

Rat UNC5H2 cDNA encodes a 945 amino acid residues (aa) type I membrane protein with a putative 26 aa signal peptide and 347 aa extracellular domain. The extracellular domain of rat UNC5H2 shares approximately 65%, 73% and 73% aa sequence similarity with that of rat UNC5H1, human UNC5H3 and mouse UNC5H3, respectively.

  • References:
    1. Hong, K. et al. (1999) Cell 97:927.
    2. Leonardo, E.D. et al. (1997) Nature 386:833.
    3. Culotti, J.B. and D.C. Merz (1998) Curr. Opin. Cell Biol. 10:609.
    4. Corset, V. (2000) Nature 407:747.
    5. Merz, D.C. (2001) Genetics 158:1071.
    6. Llambi, F. et al. (2001) EMBO J. 20:2715.
  • Long Name:
    Netrin Receptor UNC5B
  • Entrez Gene IDs:
    219699 (Human); 60630 (Rat)
  • Alternate Names:
    p53RDL1; p53-regulated receptor for death and life protein 1; Protein unc-5 homolog 2; Protein unc-5 homolog B; transmembrane receptor Unc5H2; unc5 (C.elegans homolog) b; unc-5 homolog 2; unc-5 homolog B (C. elegans); UNC5B; UNC5H2; UNC5H2netrin receptor UNC5B
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.
    Authors: Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K
    Nat Med, 2014;20(4):377-84.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions.
    Authors: van Gils, Janine M, Ramkhelawon, Bhama, Fernandes, Luciana, Stewart, Merran C, Guo, Liang, Seibert, Tara, Menezes, Gustavo, Cara, Denise C, Chow, Camille, Kinane, T Bernar, Fisher, Edward A, Balcells, Mercedes, Alvarez-Leite, Jacqueli, Lacy-Hulbert, Adam, Moore, Kathryn
    Arterioscler Thromb Vasc Biol, 2013;33(5):911-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy.
    Authors: Hagerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams R, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F
    EMBO J, 2013;32(5):629-44.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  4. The uncoordinated-5 homolog B receptor affects hepatic ischemia reperfusion injury.
    Authors: Konig K, Kohler D, Granja T, Jennewein C, Tran N, Mirakaj V, Krohnert F, Rosenberger P
    PLoS ONE, 2012;7(7):e41085.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  5. Netrin-1 Regulates Th1/Th2/Th17 Cytokine Production and Inflammation through UNC5B Receptor and Protects Kidney against Ischemia-Reperfusion Injury.
    Authors: Tadagavadi RK, Wang W
    J. Immunol., 2010;185(0):3750-8.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  6. Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia.
    Authors: Hoang S, Liauw J, Choi M
    J. Cereb. Blood Flow Metab., 2009;29(2):385-97.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Netrin-1 overexpression protects kidney from ischemia reperfusion injury by suppressing apoptosis.
    Authors: Wang W, Reeves WB, Pays L, Mehlen P, Ramesh G
    Am. J. Pathol., 2009;175(3):1010-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. Netrin-1 inhibits leukocyte migration in vitro and in vivo.
    Authors: Ly NP, Komatsuzaki K, Fraser IP, Tseng AA, Prodhan P, Moore KJ, Kinane TB
    Proc. Natl. Acad. Sci. U.S.A., 2005;102(41):14729-34.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
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