Rat UNC5H2/UNC5B Antibody
Rat UNC5H2/UNC5B Antibody Summary
Gly27-Asp373
Accession # O08722
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Rat UNC5H2 by Western Blot. Western blot shows lysates of rat spinal cord tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Rat UNC5H2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1006) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for UNC5H2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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UNC5H2/UNC5B in Rat Brain. UNC5H2/UNC5B was detected in perfusion fixed paraffin-embedded sections of rat brain using Goat Anti-Rat UNC5H2/UNC5B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1006) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: UNC5H2/UNC5B
Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues (including rat UNC5H1 and H2, mouse UNC5H2 and H3 (also known as rostral cerebellar malformation, RCM), and human UNC5H3 and H4) are transmembrane proteins belonging to the immunoglobulin (Ig) superfamily. All UNC5 family members have two Ig and two thrombospondin type 1 domains in their extracellular regions, as well as a conserved ZU-5 domain, a DCC (Deleted in Colorectal Cancer)-binding domain (DB) and a C-terminal death domain (DD) in their cytoplasmic regions (1, 2).
UNC5 family proteins are receptors for the netrin/UNC6 (netr: Sanskrit for “one who guides”) family of secreted axon guidance cues that are laminin-related proteins. Netrin family proteins can act as a chemoattractant for some axons and as a chemorepellent for others. Besides UNC5, netrin family proteins also bind to the DCC family of type I transmembrane receptors that share sequence similarity with proteins of the NCAM family, and adenosine A2b receptor, a G protein-coupled seven‑transmembrane receptor belonging to the adenosine receptor family (3, 4). In vitro, netrin binding to DCC family receptors in the absence of UNC5 is associated with axon attraction. However, the DCC-mediated attraction to netrin is converted to repulsion by binding of UNC5 to the DCC-netrin complex. In vivo, the mechanisms of netrin-dependent axon attraction and repulsion are more complex and may include UNC5-mediated repulsion that is independent of DCC (1, 5). Besides their roles in axon guidance and neuronal migration, the UNC5 and DCC families also act as dependence receptors and exert pro-apoptotic effects in the absence of netrin (6).
Rat UNC5H2 cDNA encodes a 945 amino acid residues (aa) type I membrane protein with a putative 26 aa signal peptide and 347 aa extracellular domain. The extracellular domain of rat UNC5H2 shares approximately 65%, 73% and 73% aa sequence similarity with that of rat UNC5H1, human UNC5H3 and mouse UNC5H3, respectively.
- Hong, K. et al. (1999) Cell 97:927.
- Leonardo, E.D. et al. (1997) Nature 386:833.
- Culotti, J.B. and D.C. Merz (1998) Curr. Opin. Cell Biol. 10:609.
- Corset, V. (2000) Nature 407:747.
- Merz, D.C. (2001) Genetics 158:1071.
- Llambi, F. et al. (2001) EMBO J. 20:2715.
Product Datasheets
Product Specific Notices
This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending.
Citations for Rat UNC5H2/UNC5B Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Robo4 is constitutively shed by ADAMs from endothelial cells and the shed Robo4 functions to inhibit Slit3-induced angiogenesis
Authors: W Xiao, A Pinilla-Ba, J Faulkner, X Song, P Prabhakar, H Qiu, KW Moremen, A Ludwig, PJ Dempsey, P Azadi, L Wang
Scientific Reports, 2022-03-14;12(1):4352.
Species: Human, Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: Cell Migration, Neutralization, Western Blot -
Endothelial Unc5B controls blood-brain barrier integrity
Authors: K Boyé, LH Geraldo, J Furtado, L Pibouin-Fr, M Poulet, D Kim, B Nelson, Y Xu, L Jacob, N Maissa, D Agalliu, L Claesson-W, SL Ackerman, A Eichmann
Nature Communications, 2022-03-04;13(1):1169.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Immunoprecipitation -
Macrophage-derived netrin-1 promotes abdominal aortic aneurysm formation by activating MMP3 in vascular smooth muscle cells
Authors: T Hadi, L Boytard, M Silvestro, D Alebrahim, S Jacob, J Feinstein, K Barone, W Spiro, S Hutchison, R Simon, D Rateri, F Pinet, D Fenyo, M Adelman, KJ Moore, HK Eltzschig, A Daugherty, B Ramkhelawo
Nat Commun, 2018-11-27;9(1):5022.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Netrin 1 mediates protective effects exerted by insulin-like growth factor 1 on cochlear hair cells
Authors: K Yamahara, T Nakagawa, J Ito, K Kinoshita, K Omori, N Yamamoto
Neuropharmacology, 2017-04-01;119(0):26-39.
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay -
Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.
Authors: Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K
Nat Med, 2014-03-02;20(4):377-84.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions.
Authors: van Gils, Janine M, Ramkhelawon, Bhama, Fernandes, Luciana, Stewart, Merran C, Guo, Liang, Seibert, Tara, Menezes, Gustavo, Cara, Denise C, Chow, Camille, Kinane, T Bernar, Fisher, Edward A, Balcells, Mercedes, Alvarez-Leite, Jacqueli, Lacy-Hulbert, Adam, Moore, Kathryn
Arterioscler Thromb Vasc Biol, 2013-02-21;33(5):911-9.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy.
Authors: Hagerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams R, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F
EMBO J, 2013-01-08;32(5):629-44.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
The uncoordinated-5 homolog B receptor affects hepatic ischemia reperfusion injury.
Authors: Konig K, Kohler D, Granja T, Jennewein C, Tran N, Mirakaj V, Krohnert F, Rosenberger P
PLoS ONE, 2012-07-25;7(7):e41085.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Netrin-1 Regulates Th1/Th2/Th17 Cytokine Production and Inflammation through UNC5B Receptor and Protects Kidney against Ischemia-Reperfusion Injury.
Authors: Tadagavadi RK, Wang W
J. Immunol., 2010-08-06;185(0):3750-8.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Netrin-1 overexpression protects kidney from ischemia reperfusion injury by suppressing apoptosis.
Authors: Wang W, Reeves WB, Pays L, Mehlen P, Ramesh G
Am. J. Pathol., 2009-08-21;175(3):1010-8.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia.
Authors: Hoang S, Liauw J, Choi M
J. Cereb. Blood Flow Metab., 2008-11-05;29(2):385-97.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Netrin-1 inhibits leukocyte migration in vitro and in vivo.
Authors: Ly NP, Komatsuzaki K, Fraser IP, Tseng AA, Prodhan P, Moore KJ, Kinane TB
Proc. Natl. Acad. Sci. U.S.A., 2005-10-03;102(41):14729-34.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
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