TGF-beta 2 Antibody

Catalog # Availability Size / Price Qty
AB-12-NA
Detection  of Human TGF‑ beta 2 by Western Blot.
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Product Details
Citations (5)
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TGF-beta 2 Antibody Summary

Specificity
Detects TGF-beta 2 and TGF-beta 1.2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with TGF-beta 3 and TGF-beta 1 is observed.
Source
Polyclonal Rabbit IgG
Purification
Protein A or G purified
Immunogen
Porcine platelet-derived TGF-beta 2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
5 µg/mL
See below
Neutralization
Measured by its ability to neutralize TGF‑ beta 2 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line [Tsang, M. et al. (1995) Cytokine 7:389]. The Neutralization Dose (ND50) is typically 0.25-1.25 µg/mL in the presence of 1 ng/mL Porcine TGF‑ beta 2 and 7.5 ng/mL Recombinant Mouse IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human TGF‑ beta 2 by Western Blot. View Larger

Detection of Human TGF‑ beta 2 by Western Blot. Western blot shows lysates of human heart tissue and human breast cancer tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-TGF‑ beta 2 Polyclonal Antibody (Catalog # AB-12-NA) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for TGF‑ beta 2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection  of Human TGF‑ beta 2 by Simple WesternTM. View Larger

Detection of Human TGF‑ beta 2 by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue and human breast cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for TGF‑ beta 2 at approximately 64 kDa (as indicated) using 5 µg/mL of Rabbit Anti-TGF‑ beta 2 Polyclonal Antibody
(Catalog # AB-12-NA). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.        

Neutralization TGF‑ beta 2 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 2 Antibody. View Larger

TGF‑ beta 2 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 2 Antibody. Porcine TGF‑ beta 2 (Catalog # 102-B2) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL‑4 (7.5 ng/mL) activity elicited by Porcine TGF‑ beta 2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-TGF‑ beta 2 Polyclonal Antibody (Catalog # AB-12-NA). The ND50 is typically
0.25-1.25 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 2

TGF-beta 2 (transforming growth factor beta 2) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cysteine knot structure (1-7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1-4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 2, mice with targeted deletion show defects in development of cardiac, lung, craniofacial, limb, eye, ear and urogenital systems (2). Human TGF-beta 2 cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal peptide and a 395 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 232 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 2 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex (8-10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 2 shows 100% aa identity with porcine, canine, equine and bovine TGF-beta 2, and 97% aa identity with mouse and rat TGF-beta 2. It demonstrates cross-species activity (1). TGF-beta 2 signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates another ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1. The whole complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Use of other signaling pathways that are Smad-independent allows for disparate actions observed in response to TGF-beta in different contexts (11).

References
  1. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
  2. Dunker, N. and K. Krieglstein, 2000, Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. deMartin, R. et al. (1987) EMBO J. 6:3673.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
Long Name
Transforming Growth Factor beta 2
Entrez Gene IDs
7042 (Human); 21808 (Mouse); 397084 (Porcine)
Alternate Names
BSC-1 cell growth inhibitor; cetermin; Glioblastoma-derived T-cell suppressor factor; G-TSF; MGC116892; polyergin; TGFB2; TGFbeta 2; TGF-beta 2; TGF-beta2; TGF-beta-2; transforming growth factor beta-2; transforming growth factor, beta 2

Product Datasheets

Citations for TGF-beta 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. MicroRNA-30b controls endothelial cell capillary morphogenesis through regulation of transforming growth factor beta 2
    Authors: GA Howe, K Kazda, CL Addison
    PLoS ONE, 2017;12(10):e0185619.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  2. Tartrate-resistant acid phosphatase (TRAP/ACP5) promotes metastasis-related properties via TGF?2/T?R and CD44 in MDA-MB-231 breast cancer cells
    Authors: A Reithmeier, E Panizza, M Krumpel, LM Orre, RMM Branca, J Lehtiö, B Ek-Rylande, G Andersson
    BMC Cancer, 2017;17(1):650.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  3. Cancer associated fibroblasts regulate keratinocyte cell-cell adhesion via TGF-�-dependent pathways in genotype-specific oral cancer
    Authors: S S Prime
    Carcinogenesis, 2016;0(0):.
    Species: Human
    Sample Types: Conditioned Media
    Applications: Neut
  4. Differential versican isoforms and aggrecan expression in the chicken embryo aorta.
    Authors: Arciniegas E, Neves CY, Candelle D, Parada D
    Anat Rec A Discov Mol Cell Evol Biol, 2004;279(1):592-600.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC
  5. Isoform specificity of commercially-available anti-TGF-beta antibodies.
    Authors: Mozes MM, Hodics T, Kopp JB
    J. Immunol. Methods, 1999;225(1):87-93.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: WB

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TGF-beta 2 Antibody
By Anonymous on 05/02/2016
Application: WB Sample Tested: colon cancer cell lysate Species: Human