TGF‑ beta 2 Antibody

R&D Systems | Catalog # AB-12-NA

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Porcine, Avian - Chicken

Applications

Validated:

Western Blot, Neutralization, Simple Western

Cited:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, ELISA Detection

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all TGF-beta 2 Primary Antibodies »

Product Specifications

Immunogen

Porcine platelet-derived TGF-beta 2

Specificity

Detects TGF-beta 2 and TGF-beta 1.2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with TGF-beta 3 and TGF-beta 1 is observed.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for TGF‑ beta 2 Antibody

Detection of Human TGF-beta 2 antibody by Western Blot.

Detection of Human TGF‑ beta 2 by Western Blot.

Western blot shows lysates of human heart tissue and human breast cancer tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-TGF-beta 2 Polyclonal Antibody (Catalog # AB-12-NA) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for TGF-beta 2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human TGF-beta 2 antibody by Simple WesternTM.

Detection of Human TGF‑ beta 2 by Simple WesternTM.

Simple Western lane view shows lysates of human heart tissue and human breast cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for TGF‑ beta 2 at approximately 64 kDa (as indicated) using 5 µg/mL of Rabbit Anti-TGF‑ beta 2 Polyclonal Antibody (Catalog # AB-12-NA). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
TGF‑ beta 2 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 2 Antibody.

TGF‑ beta 2 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 2 Antibody.

Porcine TGF-beta 2 (Catalog # 102-B2) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Porcine TGF-beta 2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-TGF-beta 2 Polyclonal Antibody (Catalog # AB-12-NA). The ND50 is typically 0.25-1.25 µg/mL.

Detection of Human TGF-beta 2 Antibody by Western Blot

Detection of Human TGF-beta 2 Antibody by Western Blot

MiR-30b induces expression of TGF beta 2.(A) HUVECs were transfected with either control mimic (con) or miR-30b mimic (30b) and levels of TGF beta 1 and TGF beta 2 mRNA were assessed by qRT-PCR. Expression levels relative to control mimic transfected cells and normalized to beta -actin expression are presented as the mean ± SEM (n = 2). Overexpression of miR-30b significantly increases TGF beta 2 expression. * P < 0.05, ** P < 0.01 as determined by unpaired Student’s t-test. (B) Cells were transfected with 20 nM of either control mimic (control) or miR-30b mimic (miR-30b) and protein lysates were collected after 48 hours for assessment of TGF beta 2 protein levels by western blot. beta -actin was used as endogenous control. (C) ELISAs for TGF beta 1 and TGF beta 2 were performed with 24 hour conditioned supernates from HUVECs transfected with 20 nM of either control or miR-30b mimic. Data represents the mean ± SEM (n = 2). Overexpression of miR-30b significantly increases TGF beta 2 secretion into cell culture supernate. * P = 0.044 as determined by unpaired Student’s t-test. (D) HUVECs were transfected with 20 nM of either control mimic (control) or miR-30b mimic (miR-30b) and protein lysates were collected after 48 hours for assessment of Smad2 phosphorylation by western blot. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28977001), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human TGF-beta 2 Antibody by Western Blot

Detection of Human TGF-beta 2 Antibody by Western Blot

MiR-30b induces expression of TGF beta 2.(A) HUVECs were transfected with either control mimic (con) or miR-30b mimic (30b) and levels of TGF beta 1 and TGF beta 2 mRNA were assessed by qRT-PCR. Expression levels relative to control mimic transfected cells and normalized to beta -actin expression are presented as the mean ± SEM (n = 2). Overexpression of miR-30b significantly increases TGF beta 2 expression. * P < 0.05, ** P < 0.01 as determined by unpaired Student’s t-test. (B) Cells were transfected with 20 nM of either control mimic (control) or miR-30b mimic (miR-30b) and protein lysates were collected after 48 hours for assessment of TGF beta 2 protein levels by western blot. beta -actin was used as endogenous control. (C) ELISAs for TGF beta 1 and TGF beta 2 were performed with 24 hour conditioned supernates from HUVECs transfected with 20 nM of either control or miR-30b mimic. Data represents the mean ± SEM (n = 2). Overexpression of miR-30b significantly increases TGF beta 2 secretion into cell culture supernate. * P = 0.044 as determined by unpaired Student’s t-test. (D) HUVECs were transfected with 20 nM of either control mimic (control) or miR-30b mimic (miR-30b) and protein lysates were collected after 48 hours for assessment of Smad2 phosphorylation by western blot. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28977001), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for TGF‑ beta 2 Antibody

Application
Recommended Usage

Simple Western

5 µg/mL
Sample: Human heart tissue and human breast cancer tissue

Western Blot

0.1 µg/mL
Sample: Human heart tissue and human breast cancer tissue

Neutralization

Measured by its ability to neutralize TGF‑ beta 2 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line [Tsang, M. et al. (1995) Cytokine 7:389]. The Neutralization Dose (ND50) is typically 0.25-1.25 µg/mL in the presence of 1 ng/mL Porcine TGF‑ beta 2 and 7.5 ng/mL Recombinant Mouse IL‑4.

Reviewed Applications

Read 1 review rated 3 using AB-12-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta 2

TGF-beta 2 (transforming growth factor beta 2) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cysteine knot structure (1-7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1-4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 2, mice with targeted deletion show defects in development of cardiac, lung, craniofacial, limb, eye, ear and urogenital systems (2). Human TGF-beta 2 cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal peptide and a 395 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 232 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 2 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex (8-10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 2 shows 100% aa identity with porcine, canine, equine and bovine TGF-beta 2, and 97% aa identity with mouse and rat TGF-beta 2. It demonstrates cross-species activity (1). TGF-beta 2 signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates another ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1. The whole complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Use of other signaling pathways that are Smad-independent allows for disparate actions observed in response to TGF-beta in different contexts (11).

References

  1. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
  2. Dunker, N. and K. Krieglstein, 2000, Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. deMartin, R. et al. (1987) EMBO J. 6:3673.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

Long Name

Transforming Growth Factor beta 2

Alternate Names

TGFB2, TGFbeta 2

Entrez Gene IDs

7042 (Human); 21808 (Mouse); 397084 (Porcine)

Gene Symbol

TGFB2

Additional TGF-beta 2 Products

Product Documents for TGF‑ beta 2 Antibody

Certificate of Analysis

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Product Specific Notices for TGF‑ beta 2 Antibody

For research use only

Citations for TGF‑ beta 2 Antibody

Customer Reviews for TGF‑ beta 2 Antibody (1)

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  • TGF-beta 2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: colon cancer cell lysate
    Species: Human
    Verified Customer | Posted 05/02/2016
    TGF‑ beta 2 Antibody AB-12-NA

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