TGF-beta 2 Antibody

(2 citations)
(1 Review)
  
  • Specificity
    Detects TGF-beta 2 and TGF-beta 1.2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with TGF-beta 3 and TGF-beta 1 is observed.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Protein A or G purified
  • Immunogen
    Porcine platelet-derived TGF-beta 2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize TGF‑ beta 2 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line [Tsang, M. et al. (1995) Cytokine 7:389]. The Neutralization Dose (ND50) is typically 0.25-1.25 µg/mL in the presence of 1 ng/mL Porcine TGF‑ beta 2 and 7.5 ng/mL Recombinant Mouse IL‑4.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human TGF‑ beta 2 by Western Blot. Western blot shows lysates of human heart tissue and human breast cancer tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-TGF‑ beta 2 Polyclonal Antibody (Catalog # AB-12-NA) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for TGF‑ beta 2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human TGF‑ beta 2 by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue and human breast cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for TGF‑ beta 2 at approximately 64 kDa (as indicated) using 5 µg/mL of Rabbit Anti-TGF‑ beta 2 Polyclonal Antibody
(Catalog # AB-12-NA). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.        
TGF‑ beta 2 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 2 Antibody. Porcine TGF‑ beta 2 (Catalog # 102-B2) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL‑4 (7.5 ng/mL) activity elicited by Porcine TGF‑ beta 2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-TGF‑ beta 2 Polyclonal Antibody (Catalog # AB-12-NA). The ND50 is typically
0.25-1.25 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 1 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta 2
TGF-beta 2 (transforming growth factor beta 2) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cysteine knot structure (1-7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1-4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 2, mice with targeted deletion show defects in development of cardiac, lung, craniofacial, limb, eye, ear and urogenital systems (2). Human TGF-beta 2 cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal peptide and a 395 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 232 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 2 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex (8-10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 2 shows 100% aa identity with porcine, canine, equine and bovine TGF-beta 2, and 97% aa identity with mouse and rat TGF-beta 2. It demonstrates cross-species activity (1). TGF-beta 2 signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates another ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1. The whole complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Use of other signaling pathways that are Smad-independent allows for disparate actions observed in response to TGF-beta in different contexts (11).
  • References:
    1. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
    2. Dunker, N. and K. Krieglstein, 2000, Eur. J. Biochem. 267:6982.
    3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
    4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
    5. Lin, J.S. et al. (2006) Reproduction 132:179.
    6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
    7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
    8. deMartin, R. et al. (1987) EMBO J. 6:3673.
    9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
    10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
    11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
    12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
  • Long Name:
    Transforming Growth Factor beta 2
  • Entrez Gene IDs:
    7042 (Human); 21808 (Mouse); 397084 (Porcine)
  • Alternate Names:
    BSC-1 cell growth inhibitor; cetermin; Glioblastoma-derived T-cell suppressor factor; G-TSF; MGC116892; polyergin; TGFB2; TGFbeta 2; TGF-beta 2; TGF-beta2; TGF-beta-2; transforming growth factor beta-2; transforming growth factor, beta 2
Related Research Areas
Citations:

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2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Differential versican isoforms and aggrecan expression in the chicken embryo aorta.
    Authors: Arciniegas E, Neves CY, Candelle D, Parada D
    Anat Rec A Discov Mol Cell Evol Biol, 2004;279(1):592-600.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: IHC
  2. Isoform specificity of commercially-available anti-TGF-beta antibodies.
    Authors: Mozes MM, Hodics T, Kopp JB
    J. Immunol. Methods, 1999;225(1):87-93.
    Species: Human
    Sample Type: Recombinant Protein
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 43  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Rabbit IgG Antibody

WB AF008 1
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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Average Rating: 3 (Based on 1 review)

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We have 1 review tested in 1 application: Western Blot.

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Western Blot TGF-beta 2 Antibody AB-12-NA
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 WB Human Anonymous 05/02/2016
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Western Blot TGF-beta 2 Antibody AB-12-NA
Western Blot: TGF-beta 2 Antibody [AB-12-NA]

Summary

ApplicationWestern Blot
Sample Testedcolon cancer cell lysate
SpeciesHuman

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