Viral Chikungunya Virus E1 Antibody

MAB9779 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9779-100
MAB9779-SP

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Viral Chikungunya Virus E1 ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Viral Chikungunya Virus E1 Antibody Summary

Species Reactivity

Viral

Specificity

Detects viral Chikungunya Virus E1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 988110

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell line HEK293-derived transfected with viral Chikungunya Virus E1
Tyr810-Gly1224
Accession # Q8JUX5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Viral Chikungunya Virus E1 Monoclonal Antibody (Catalog # MAB97791).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

ELISA

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Viral Chikungunya Virus E1 ELISA Standard Curve. Recombinant Viral Chikungunya Virus E1 protein was serially diluted 2-fold and captured by Mouse Anti-Viral Chikungunya Virus E1 Monoclonal Antibody (Catalog # MAB97791) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral Chikungunya Virus E1 Monoclonal Antibody (Catalog # MAB9779) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chikungunya Virus E1

Chikungunya fever is a pandemic disease caused by the flavivirus Chikungunya (CHIKV). The 47 kDa E1 glycoprotein mediates viral membrane fusion during CHIKV infection. The CHIKV envelope protein E1 is a component of the viral spike, which is composed of triplets of heterodimer of E1 and E2 glycoproteins, expressed on the viral surface. The viral spike proteins facilitate attachment to cell surfaces and viral entry into the cells. The E1 envelope protein is a class II fusion protein that mediates low pH-triggered membrane fusion during virus infection.

Long Name

Chikungunya Virus Envelope 1

Entrez Gene IDs

956308 (Viral)

Alternate Names

CHICKV E1; Chikungunya Virus E1; Chikungunya Virus; CHIKV-E1