CyTOF®, also known as mass cytometry, takes advantage of the low spectral overlap between heavy metal isotopes. In this technique, heavy metals are conjugated to antibodies, and their unique mass signatures are detected by time-of-flight mass spectrometry. Heavy metals take the place of fluorophores which are relatively limited by their overlap in fluorescence emission spectra. This allows the simultaneous detection and resolution of more molecular targets than are practical with the use of fluorescent flow cytometry dyes. Over 30 cellular targets can be identified on a single cell without the need for fluorescence compensation adjustments.
Benefits of R&D Systems antibodies for established conjugation methods
Lyophilized for reconstitution at high concentration and improved conjugation efficiency
No BSA or other carrier proteins that could interfere with conjugation
No buffer exchange step required before conjugation
Can also be conjugated to fluorochromes for traditional flow cytometry
R&D Systems antibodies that have been published for their use in CyTOF analysis.