Antibodies for CyTOF

CyTOF®, also known as mass cytometry, takes advantage of the low spectral overlap between heavy metal isotopes. In this technique, heavy metals are conjugated to antibodies, and their unique mass signatures are detected by time-of-flight mass spectrometry. Heavy metals take the place of fluorophores which are relatively limited by their overlap in fluorescence emission spectra. This allows the simultaneous detection and resolution of more molecular targets than are practical with the use of fluorescent flow cytometry dyes. Over 30 cellular targets can be identified on a single cell without the need for fluorescence compensation adjustments.

Benefits of R&D Systems antibodies for established conjugation methods

  • Lyophilized for reconstitution at high concentration and improved conjugation efficiency
  • No BSA or other carrier proteins that could interfere with conjugation
  • No buffer exchange step required before conjugation
  • Can also be conjugated to fluorochromes for traditional flow cytometry

R&D Systems antibodies that have been published for their use in CyTOF analysis.

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R&D Systems antibodies lyophilized in a CyTOF-ready format

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Human CCR10 Monoclonal Antibody, Catalog # MAB3478

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Recent Citations:

High-dimensional immune profiling of total and rotavirus VP6-specific intestinal and circulating B cells by mass cytometry. Nair, N. et al. Mucosal Immunol. (2016) 9: 68. Catalog #s: MAB197, MAB170

Immune profiles to predict response to desensitization therapy in highly HLA-sensitized kidney transplant candidates. Yabu, J.M. et al. PLoS ONE (2016) 11: e0153355. Catalog #s: MAB197, MAB20172

Barcoding of live human peripheral blood mononuclear cells for multiplexed mass cytometry. Mei, H. et al. J Immunol, (2015) 194:2022. Catalog #s: MAB20172, MAB197

Regulation of Adaptive NK Cells and CD8 T Cells by HLA-C Correlates with Allogeneic Hematopoietic Cell Transplantation and with Cytomegalovirus Reactivation. Horowitz, A. et al. J Immunol (2015) 195:4524. Catalog #s: MAB138, MAB1844, MAB2014

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