Human LILRB1/CD85j/ILT2 Antibody

Catalog # Availability Size / Price Qty
MAB20172
MAB20172-SP
Cell Adhesion Mediated by LILRB1/CD85j/ILT2 and Neutralization by Human LILRB1/CD85j/ILT2 Antibody.
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Citations (5)
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Human LILRB1/CD85j/ILT2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LILRB1/CD85j/ILT2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ILT1, 3, 4, 5, rhLIR-6 or rhLIR-8 is observed.
Source
Monoclonal Mouse IgG2B Clone # 292319
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LILRB1/CD85j/ILT2
Gly24-His458
Accession # Q8NHL6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (Catalog # 2017-T2)
CyTOF-reported
Yabu, J.M. et al. (2016) PLoS ONE 11:e0153355. Ready to be labeled using established conjugation methods. NoBSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize LILRB1/CD85j/ILT2-mediated adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line. Cosman, D. et al. (1997) Immunity 7:273. The Neutralization Dose (ND50) is typically 0.025-0.15 µg/mL in the presence of 10 µg/mL Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Adhesion Mediated by LILRB1/CD85j/ILT2 and Neutralization by Human LILRB1/CD85j/ILT2 Antibody. View Larger

Cell Adhesion Mediated by LILRB1/CD85j/ILT2 and Neutralization by Human LILRB1/CD85j/ILT2 Antibody. Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (Catalog # 2017-T2), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent manner (orange line), as measured by acid phosphatase activity. Adhesion elicited by Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human LILRB1/CD85j/ILT2 Monoclonal Antibody (Catalog # MAB20172). The ND50 is typically 0.025‑0.15 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRB1/CD85j/ILT2

The immunoglobulin-like transcript (ILT) family of activating and inhibitory type immunoreceptors are expressed on many leukocyte subsets and function in the regulation of immune responses (1‑3). This family was also named leukocyte Ig-like receptors (LIR) and monocyte/macrophage Ig-like receptors (MIR). ILTs share significant homology with killer cell Ig-like receptors (KIR). The ILT genes are located on human chromosome 19q13.4 in the leukocyte receptor complex, which also include the genes encoding KIRs (4). With the exception of ILT-6, which is a soluble molecule, all ILT family members are type I transmembrane proteins having two or four extracellular Ig-like domains (2, 3). One subset of the ILT receptors (referred to as subfamily B of the LIRs) has long cytoplasmic tails containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events by recruiting SH2-containing protein tyrosine phosphatase-1. Another subset of the ILT receptors (referred to as subfamily A of the LIRs) contains activating receptors with short cytoplasmic regions that lack signal transduction motifs. These receptors contain a basic arginine residue within their transmembrane domains, which allows association with Fc R gamma, an immunoreceptor tyrosine-based activation motif (ITAM)-bearing signal adapter protein (1‑3). ILT2, also known as LIR1, MIR7, and CD85j, is expressed on most monocytes, dendritic cells, and mature B cells (1‑3). It is also expressed on small percentages of T cells and NK cells. ILT2 has four extracellular Ig-like domains and three cytoplasmic ITIMs. It functions as an inhibitory receptor that prevents cellular activation. ILT2 has been shown to bind classical (HLA-A and -B) and nonclassical (HLA-G1, -E and -F) MHC class I molecules (MHCI) (1‑3). ILT2 also binds with high affinity to an MHC class I homologue from human cytomegalovirus (3). Ligation of ILT2 by MHC class I may function to poise cellular activation thresholds and inhibit various leukocyte effector mechanisms that are regulated by MHC class I molecules on target cells.

References
  1. Allen, D. et al. (2000) Immunobiol. 202:34.
  2. Colonna, M. et al. (1999) J. Leukocyte Biol. 66:375.
  3. Borges, L. and D. Cosman (2000) Cytokine Growth Factor Rev. 11:209.
  4. Young, N. et al. (2001) Immunogenetics 53:270.
Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 1
Entrez Gene IDs
10859 (Human)
Alternate Names
CD85 antigen-like family member J; CD85j antigen; CD85j; Ig-like transcript 2; ILT2; ILT-2; ILT2FLJ37515; Immunoglobulin-like transcript 2; leukocyte Ig-like receptor-1; Leukocyte immunoglobulin-like receptor 1; leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform; leukocyte immunoglobulin-like receptor subfamily B member 1; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB1; LIR1; LIR1LIR-1MIR-7CD85MIR7CD85 antigen; member 1; MIR7; Monocyte/macrophage immunoglobulin-like receptor 7

Product Datasheets

Citations for Human LILRB1/CD85j/ILT2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Reconstitution of immune cell populations in multiple sclerosis patients after autologous stem cell transplantation
    Authors: FG Karnell, D Lin, S Motley, T Duhen, N Lim, DJ Campbell, LA Turka, HT Maecker, KM Harris
    Clin. Exp. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof
  2. Barcoding of live human peripheral blood mononuclear cells for multiplexed mass cytometry.
    Authors: Mei H, Leipold M, Schulz A, Chester C, Maecker H
    J Immunol, 2015;194(4):2022-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof
  3. Inhibitory receptors are expressed by Trypanosoma cruzi-specific effector T cells and in hearts of subjects with chronic Chagas disease.
    Authors: Arguello RJ, Albareda MC, Alvarez MG, Bertocchi G, Armenti AH, Vigliano C, Meckert PC, Tarleton RL, Laucella SA
    PLoS ONE, 2012;7(5):e35966.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  4. Natural killer cell killing of acute myelogenous leukemia and acute lymphoblastic leukemia blasts by killer cell immunoglobulin-like receptor-negative natural killer cells after NKG2A and LIR-1 blockade.
    Authors: Godal R, Bachanova V, Gleason M
    Biol. Blood Marrow Transplant., 2010;16(5):612-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  5. Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation.
    Authors: Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B
    Blood, 2007;109(7):2806-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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