Key Product Details

Species Reactivity

Cotton Rat

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 695542
View all CD4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant cotton rat CD4
Lys26-Pro393

Specificity

Detects cotton rat CD4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant CD4 from human, mouse, rat, cat, or dog is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Cotton Rat CD4 Antibody

Detection of CD4 antibody in Cotton Rat Splenocytes antibody by Flow Cytometry.

Detection of CD4 in Cotton Rat Splenocytes by Flow Cytometry.

Cotton rat splenocytes were stained with Mouse Anti-Cotton Rat CD4 Monoclonal Antibody (Catalog # MAB6676, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Cotton Rat CD4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Cotton rat splenocytes

Reviewed Applications

Read 1 review rated 1 using MAB6676 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD4

CD4 is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and the Th1, Th2, Th17, Tfh, and Treg subsets of mature T lymphocytes. In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain. CD4 binding to MHC class II molecules on antigen presenting cells contributes to the formation of the immunological synapse. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a coreceptor for the gp120 surface glycoprotein of HIV-1.

Alternate Names

CD4

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Gene Symbol

CD4

Additional CD4 Products

Product Documents for Cotton Rat CD4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Cotton Rat CD4 Antibody

For research use only

Customer Reviews for Cotton Rat CD4 Antibody (1)

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Customer Images

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  • Cotton Rat CD4 Antibody
    Name: Magdalena Migalska
    Application: Flow Cytometry
    Sample Tested: Thymocytes
    Species: Bank vole (Myodes glareolus)
    Verified Customer | Posted 06/30/2020
    Bank vole thymocytes. Gating – lymphocytes. A- no staining. B- control staining – only 2nd-ry mAb. C- primary anti-Cotton Rat CD4 Antibody + 2nd-ry antibody. D- gating – all events, overlay: black – as in A, blue – as in B, green – as in C.
    Test of cross-reactivity of antibodies between closely related species - the cotton rat and the bank vole; Family: Cricetidae. Bank vole thymocytes were stained with Mouse Anti-Cotton Rat CD4 Monoclonal Antibody (Cat # MAB6676);, followed by FITC-conjugated Anti-Mouse IgG Secondary Antibody. Cell were analyzed with CytoFLEX (Beckman Coulter). The clone 695542 does not cross-react with bank vole CD4.
    Cotton Rat CD4 Antibody MAB6676
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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