Cotton Rat IL-1 beta/IL-1F2 Antibody

Name: Cotton Rat IL-1 beta/IL-1F2 Antibody
Brand: R&D Systems
SKU: AF1009
Price: 439 USD
Availability: InStock

R&D Systems | Catalog # AF1009

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Cotton Rat

Applications

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all IL-1 beta/IL-1F2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant cotton rat IL‑1 beta

Specificity

Detects cotton rat IL‑1 beta /IL‑1F2 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human IL-1 beta and recombinant mouse IL-1 beta is observed, and 10% cross‑reactivity with recombinant rat IL-1 beta and recombinant porcine IL-1 beta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Cotton Rat IL-1 beta/IL-1F2 Antibody

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Cotton Rat IL‑1 beta / IL‑1F2 Antibody.

Recombinant Cotton Rat IL-1 beta /IL-1F2 (1009-CL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-1 beta /IL-1F2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL-1 beta / IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1009). The ND₅₀ is typically 0.4-1.2 µg/mL.

Applications for Cotton Rat IL-1 beta/IL-1F2 Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Cotton Rat IL‑1 beta /IL‑1F2 (Catalog # 1009-CL)

Neutralization

Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 0.4-1.2 µg/mL in the presence of 2 ng/mL Recombinant Cotton Rat IL‑1 beta /IL‑1F2.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in cotton rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1‑4). The cotton rat IL-1 beta cDNA encodes a 267 aa precursor. A 115 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature cotton rat IL-1 beta shares 90% aa sequence identity with mouse and rat and 65%‑78% with canine, equine, feline, human, porcine, and rhesus IL-1 beta.